Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol
The misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-react...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1499709 |
| _version_ | 1818394677597962240 |
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| author | Wenliang Ge Steven Suryoprabowo Xiaoling Wu Qiankun Zheng Hua Kuang |
| author_facet | Wenliang Ge Steven Suryoprabowo Xiaoling Wu Qiankun Zheng Hua Kuang |
| author_sort | Wenliang Ge |
| collection | DOAJ |
| description | The misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-reactivity in pig urine. For the immunochromatographic test, the coating antigen which was obtained by conjugating the salbutamol to bovine serum albumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the control line and test line. The test was developed with a sensitive monoclonal antibody specific for MAB, which was generated by immunizing BALB/c mice with a well-characterized MAB- keyhole limpet haemocyanin conjugate, produced in our laboratory. Under optimized conditions, the cut-off limits of the semi-quantitative test strip for MBA and MPA were as low as 10 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. Our data indicate that the immunochromatographic assay allows provide sensitive, rapid, and specific on-site screening for MAB and MAP. |
| first_indexed | 2024-12-14T06:05:01Z |
| format | Article |
| id | doaj.art-c7327603943f4f5b9f8160328bbd56b9 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-14T06:05:01Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-c7327603943f4f5b9f8160328bbd56b92022-12-21T23:14:20ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-012911028104010.1080/09540105.2018.14997091499709Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterolWenliang Ge0Steven Suryoprabowo1Xiaoling Wu2Qiankun Zheng3Hua Kuang4Wuxi No. 2 people’s hospitalState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityDelishi GroupState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityThe misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-reactivity in pig urine. For the immunochromatographic test, the coating antigen which was obtained by conjugating the salbutamol to bovine serum albumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the control line and test line. The test was developed with a sensitive monoclonal antibody specific for MAB, which was generated by immunizing BALB/c mice with a well-characterized MAB- keyhole limpet haemocyanin conjugate, produced in our laboratory. Under optimized conditions, the cut-off limits of the semi-quantitative test strip for MBA and MPA were as low as 10 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. Our data indicate that the immunochromatographic assay allows provide sensitive, rapid, and specific on-site screening for MAB and MAP.http://dx.doi.org/10.1080/09540105.2018.1499709β-agonistsimmunochromatographic assaymabuterolmapenterolmonoclonal antibodysalbutamolstrip test |
| spellingShingle | Wenliang Ge Steven Suryoprabowo Xiaoling Wu Qiankun Zheng Hua Kuang Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol Food and Agricultural Immunology β-agonists immunochromatographic assay mabuterol mapenterol monoclonal antibody salbutamol strip test |
| title | Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol |
| title_full | Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol |
| title_fullStr | Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol |
| title_full_unstemmed | Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol |
| title_short | Rapid immunochromatographic test strip detection of mabuterol and its cross-reactivity with mapenterol |
| title_sort | rapid immunochromatographic test strip detection of mabuterol and its cross reactivity with mapenterol |
| topic | β-agonists immunochromatographic assay mabuterol mapenterol monoclonal antibody salbutamol strip test |
| url | http://dx.doi.org/10.1080/09540105.2018.1499709 |
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