A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects
This placebo-controlled, randomised, double-blind, cross-over human feeding study aimed to determine the prebiotic effect of agave fructans. A total of thirty-eight volunteers completed this trial. The treatment consisted of 3 weeks' supplementation with 5 g/d of prebiotic agave fructan (Predil...
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| Language: | English |
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Cambridge University Press
2015-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679014000688/type/journal_article |
| _version_ | 1811156017377968128 |
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| author | P. Ramnani A. Costabile A. G. R. Bustillo G. R. Gibson |
| author_facet | P. Ramnani A. Costabile A. G. R. Bustillo G. R. Gibson |
| author_sort | P. Ramnani |
| collection | DOAJ |
| description | This placebo-controlled, randomised, double-blind, cross-over human feeding study aimed to determine the prebiotic effect of agave fructans. A total of thirty-eight volunteers completed this trial. The treatment consisted of 3 weeks' supplementation with 5 g/d of prebiotic agave fructan (Predilife) or equivalent placebo (maltodextrin), followed by a 2-week washout period following which subjects were crossed over to alternate the treatment arm for 3 weeks followed by a 2-week washout. Faecal samples were collected at baseline, on the last day of treatment (days 22 and 58) and washout (days 36 and 72), respectively. Changes in faecal bacterial populations, SCFA and secretory IgA were assessed using fluorescent in situ hybridisation, GC and ELISA, respectively. Bowel movements, stool consistencies, abdominal comfort and mood changes were evaluated by a recorded daily questionnaire. In parallel, the effect of agave fructans on different regions of the colon using a three-stage continuous culture simulator was studied. Predilife significantly increased faecal bifidobacteria (log10 9·6 (sd 0·4)) and lactobacilli (log10 7·7 (sd 0·8)) compared with placebo (log10 9·2 (sd 0·4); P = 0·00) (log10 7·4 (sd 0·7); P = 0·000), respectively. No change was observed for other bacterial groups tested, SCFA, secretory IgA, and PGE2 concentrations between the treatment and placebo. Denaturing gradient gel electrophoresis analysis indicated that bacterial communities were randomly dispersed and no significant differences were observed between Predilife and placebo treatments. The in vitro models showed similar increases in bifidobacterial and lactobacilli populations to that observed with the in vivo trial. To conclude, agave fructans are well tolerated in healthy human subjects and increased bifidobacteria and lactobacilli numbers in vitro and in vivo but did not influence other products of fermentation. |
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| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:44:21Z |
| publishDate | 2015-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-c7342468f49f4c0db7007979109c79072023-03-09T12:38:43ZengCambridge University PressJournal of Nutritional Science2048-67902015-01-01410.1017/jns.2014.68A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjectsP. Ramnani0A. Costabile1A. G. R. Bustillo2G. R. Gibson3Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UKDepartment of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UKBustar Alimentos, SAPI de CV, Efrain Gonzalez Luna 2503, Col Arcos Sur, 44130 Guadalajara, Jalisco, MexicoDepartment of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UKThis placebo-controlled, randomised, double-blind, cross-over human feeding study aimed to determine the prebiotic effect of agave fructans. A total of thirty-eight volunteers completed this trial. The treatment consisted of 3 weeks' supplementation with 5 g/d of prebiotic agave fructan (Predilife) or equivalent placebo (maltodextrin), followed by a 2-week washout period following which subjects were crossed over to alternate the treatment arm for 3 weeks followed by a 2-week washout. Faecal samples were collected at baseline, on the last day of treatment (days 22 and 58) and washout (days 36 and 72), respectively. Changes in faecal bacterial populations, SCFA and secretory IgA were assessed using fluorescent in situ hybridisation, GC and ELISA, respectively. Bowel movements, stool consistencies, abdominal comfort and mood changes were evaluated by a recorded daily questionnaire. In parallel, the effect of agave fructans on different regions of the colon using a three-stage continuous culture simulator was studied. Predilife significantly increased faecal bifidobacteria (log10 9·6 (sd 0·4)) and lactobacilli (log10 7·7 (sd 0·8)) compared with placebo (log10 9·2 (sd 0·4); P = 0·00) (log10 7·4 (sd 0·7); P = 0·000), respectively. No change was observed for other bacterial groups tested, SCFA, secretory IgA, and PGE2 concentrations between the treatment and placebo. Denaturing gradient gel electrophoresis analysis indicated that bacterial communities were randomly dispersed and no significant differences were observed between Predilife and placebo treatments. The in vitro models showed similar increases in bifidobacterial and lactobacilli populations to that observed with the in vivo trial. To conclude, agave fructans are well tolerated in healthy human subjects and increased bifidobacteria and lactobacilli numbers in vitro and in vivo but did not influence other products of fermentation.https://www.cambridge.org/core/product/identifier/S2048679014000688/type/journal_articleAgave fructansGut microbiotaPrebiotics |
| spellingShingle | P. Ramnani A. Costabile A. G. R. Bustillo G. R. Gibson A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects Journal of Nutritional Science Agave fructans Gut microbiota Prebiotics |
| title | A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| title_full | A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| title_fullStr | A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| title_full_unstemmed | A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| title_short | A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| title_sort | randomised double blind cross over study investigating the prebiotic effect of agave fructans in healthy human subjects |
| topic | Agave fructans Gut microbiota Prebiotics |
| url | https://www.cambridge.org/core/product/identifier/S2048679014000688/type/journal_article |
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