A Comparative Study on Pseudomonal and Bacillus L. asparaginases
The enzyme L-asparaginase is commonly used in treatment of acute lymphoblastic leukemia (ALL). Due to its therapeutic importance and growing demands in medical and food industry much of the research have been focused to produce this enzyme with better activity and less disadvantages; for instance mi...
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Journal of Pure and Applied Microbiology
2019-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/a-comparative-study-on-pseudomonal-and-bacillus-l-asparaginases/ |
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| author | Nuzhath Fatima Mohd Mazhar Uddin Khan Noha Emad Khadija Al-Khadir Amany Marwan Ayesha Alvi |
| author_facet | Nuzhath Fatima Mohd Mazhar Uddin Khan Noha Emad Khadija Al-Khadir Amany Marwan Ayesha Alvi |
| author_sort | Nuzhath Fatima |
| collection | DOAJ |
| description | The enzyme L-asparaginase is commonly used in treatment of acute lymphoblastic leukemia (ALL). Due to its therapeutic importance and growing demands in medical and food industry much of the research have been focused to produce this enzyme with better activity and less disadvantages; for instance microbes have been extensively used to produce L asparaginase at affordable prices, a wide range of bacterial species have been reported that can produce high yields of this enzyme. The present study is an attempt to compare the production of L asparaginases between gram positive and gram negative bacterial species isolated from soil under same fermentation conditions. In this study the productions of L asparaginase from three species of Bacillus namely Bacillus subtilis (LC425423), Bacillus aerophilus (LC425427), Bacillus endophyticus (MG928501) and three strains of Pseudomonas aeruginosa (LC425424, LC425425, LC425426) was compared. The enzyme activity of L asparaginases produced from Bacillus species were noted as 8.5, 14.3, 7.1 (U/ml). Whereas, the enzyme activity of L asparaginase produced from Pseudomonas aeruginosa strains (LC425424, LC425425, LC425426) was noted as, 8.2, 19.4, 19.1(U/ml) respectively. In this study it was found that Pseudomonas aeruginosa strains yields was more in terms of protein concentration and enzyme activity when compared to Bacillus species. In addition this study also reports the use of modified Brain heart infusion media for screening and isolation of L asparaginase producing bacterial species. It was found that the number of colonies producing L asparaginase in modified Brain heart infusion media was comparatively same when compared to modified M9 media which is extensively used in screening of L asparaginase producers. A total of 159 L asparaginase producers were screened by using two fold dilution technique on modified BHI media while modified M9 media screened 163 colonies producing L asparaginase. |
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| format | Article |
| id | doaj.art-c7364b488011493290603004746eedc6 |
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| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-11T19:17:01Z |
| publishDate | 2019-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
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| spelling | doaj.art-c7364b488011493290603004746eedc62022-12-22T04:07:25ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-09-011331635164410.22207/JPAM.13.3.37A Comparative Study on Pseudomonal and Bacillus L. asparaginasesNuzhath Fatima0Mohd Mazhar Uddin Khan1Noha Emad2Khadija Al-Khadir3Amany Marwan4Ayesha Alvi51Department of Microbiology, Rayalaseema University, Kurnool, Andhra Pradesh, India. 2Faculty of Natural Sciences, Department of Environmental Science, Addis Ababa University, Addis Ababa, Ethiopia. 3Faculty of Applied medical College, Jazan University, K.S.A.1Department of Microbiology, Rayalaseema University, Kurnool, Andhra Pradesh, India. 4Department of Microbiology, Mumtaz PG College, Hyderabad India.3Faculty of Applied medical College, Jazan University, K.S.A. 5Former Faculty in Department of Genetics, Applied Medical College Jazan University, K.S.A. The enzyme L-asparaginase is commonly used in treatment of acute lymphoblastic leukemia (ALL). Due to its therapeutic importance and growing demands in medical and food industry much of the research have been focused to produce this enzyme with better activity and less disadvantages; for instance microbes have been extensively used to produce L asparaginase at affordable prices, a wide range of bacterial species have been reported that can produce high yields of this enzyme. The present study is an attempt to compare the production of L asparaginases between gram positive and gram negative bacterial species isolated from soil under same fermentation conditions. In this study the productions of L asparaginase from three species of Bacillus namely Bacillus subtilis (LC425423), Bacillus aerophilus (LC425427), Bacillus endophyticus (MG928501) and three strains of Pseudomonas aeruginosa (LC425424, LC425425, LC425426) was compared. The enzyme activity of L asparaginases produced from Bacillus species were noted as 8.5, 14.3, 7.1 (U/ml). Whereas, the enzyme activity of L asparaginase produced from Pseudomonas aeruginosa strains (LC425424, LC425425, LC425426) was noted as, 8.2, 19.4, 19.1(U/ml) respectively. In this study it was found that Pseudomonas aeruginosa strains yields was more in terms of protein concentration and enzyme activity when compared to Bacillus species. In addition this study also reports the use of modified Brain heart infusion media for screening and isolation of L asparaginase producing bacterial species. It was found that the number of colonies producing L asparaginase in modified Brain heart infusion media was comparatively same when compared to modified M9 media which is extensively used in screening of L asparaginase producers. A total of 159 L asparaginase producers were screened by using two fold dilution technique on modified BHI media while modified M9 media screened 163 colonies producing L asparaginase.https://microbiologyjournal.org/a-comparative-study-on-pseudomonal-and-bacillus-l-asparaginases/brain heart infusion media (bhi)all (acute lymphoblastic leukemia)l asparaginase (asp) |
| spellingShingle | Nuzhath Fatima Mohd Mazhar Uddin Khan Noha Emad Khadija Al-Khadir Amany Marwan Ayesha Alvi A Comparative Study on Pseudomonal and Bacillus L. asparaginases Journal of Pure and Applied Microbiology brain heart infusion media (bhi) all (acute lymphoblastic leukemia) l asparaginase (asp) |
| title | A Comparative Study on Pseudomonal and Bacillus L. asparaginases |
| title_full | A Comparative Study on Pseudomonal and Bacillus L. asparaginases |
| title_fullStr | A Comparative Study on Pseudomonal and Bacillus L. asparaginases |
| title_full_unstemmed | A Comparative Study on Pseudomonal and Bacillus L. asparaginases |
| title_short | A Comparative Study on Pseudomonal and Bacillus L. asparaginases |
| title_sort | comparative study on pseudomonal and bacillus l asparaginases |
| topic | brain heart infusion media (bhi) all (acute lymphoblastic leukemia) l asparaginase (asp) |
| url | https://microbiologyjournal.org/a-comparative-study-on-pseudomonal-and-bacillus-l-asparaginases/ |
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