Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats
We have recently shown that the blood cell-derived secretome of remote ischaemic (RIC)-conditioned individuals provides an external source of neuroprotection. In this study, we identified the bioactive compounds from the total proteins released by those cells. Our main strategy was to separate prote...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/10/1423 |
| _version_ | 1797474955434131456 |
|---|---|
| author | Petra Bonova Jana Koncekova Miroslava Nemethova Klaudia Petrova Martin Bona Miroslav Gottlieb |
| author_facet | Petra Bonova Jana Koncekova Miroslava Nemethova Klaudia Petrova Martin Bona Miroslav Gottlieb |
| author_sort | Petra Bonova |
| collection | DOAJ |
| description | We have recently shown that the blood cell-derived secretome of remote ischaemic (RIC)-conditioned individuals provides an external source of neuroprotection. In this study, we identified the bioactive compounds from the total proteins released by those cells. Our main strategy was to separate protein–protein complexes while maintaining their native structure and testing their bioactive properties. Subsequently, we identified up- and downregulated bioactive proteins. We uncovered two bioactive fractions composed of 18 proteins. Most of the protein peaks were unchanged; however, RIC mediated a decrease in two peaks (comprising seven proteins) and an increase in one peak (identified as haptoglobin). When focussing on the biological activity of these proteins, we found positive impacts on the regulation of cellular metabolic processes and an increase in biological processes related to the acute phase response and inflammation in the RIC-treated samples. Although we have identified the 18 proteins that exert the greatest cytoprotection, additional studies are needed to elucidate their particular function and detailed mechanisms of action. |
| first_indexed | 2024-03-09T20:37:18Z |
| format | Article |
| id | doaj.art-c7369cebde914ab4b05b704c67f438e6 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T20:37:18Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c7369cebde914ab4b05b704c67f438e62023-11-23T23:08:27ZengMDPI AGBiomolecules2218-273X2022-10-011210142310.3390/biom12101423Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned RatsPetra Bonova0Jana Koncekova1Miroslava Nemethova2Klaudia Petrova3Martin Bona4Miroslav Gottlieb5Institute of Neurobiology, Biomedical Research Center of the Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Košice, SlovakiaInstitute of Neurobiology, Biomedical Research Center of the Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Košice, SlovakiaInstitute of Neurobiology, Biomedical Research Center of the Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Košice, SlovakiaInstitute of Neurobiology, Biomedical Research Center of the Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Košice, SlovakiaDepartment of Medical Physiology, Faculty of Medicine, University of Pavol Jozef Safarik, 040 01 Košice, SlovakiaInstitute of Neurobiology, Biomedical Research Center of the Slovak Academy of Sciences, Soltesovej 4-6, 040 01 Košice, SlovakiaWe have recently shown that the blood cell-derived secretome of remote ischaemic (RIC)-conditioned individuals provides an external source of neuroprotection. In this study, we identified the bioactive compounds from the total proteins released by those cells. Our main strategy was to separate protein–protein complexes while maintaining their native structure and testing their bioactive properties. Subsequently, we identified up- and downregulated bioactive proteins. We uncovered two bioactive fractions composed of 18 proteins. Most of the protein peaks were unchanged; however, RIC mediated a decrease in two peaks (comprising seven proteins) and an increase in one peak (identified as haptoglobin). When focussing on the biological activity of these proteins, we found positive impacts on the regulation of cellular metabolic processes and an increase in biological processes related to the acute phase response and inflammation in the RIC-treated samples. Although we have identified the 18 proteins that exert the greatest cytoprotection, additional studies are needed to elucidate their particular function and detailed mechanisms of action.https://www.mdpi.com/2218-273X/12/10/1423blood cellsecretomeproteinbioactivityneurotoxicityprotection |
| spellingShingle | Petra Bonova Jana Koncekova Miroslava Nemethova Klaudia Petrova Martin Bona Miroslav Gottlieb Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats Biomolecules blood cell secretome protein bioactivity neurotoxicity protection |
| title | Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats |
| title_full | Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats |
| title_fullStr | Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats |
| title_full_unstemmed | Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats |
| title_short | Identification of Proteins Responsible for the Neuroprotective Effect of the Secretome Derived from Blood Cells of Remote Ischaemic Conditioned Rats |
| title_sort | identification of proteins responsible for the neuroprotective effect of the secretome derived from blood cells of remote ischaemic conditioned rats |
| topic | blood cell secretome protein bioactivity neurotoxicity protection |
| url | https://www.mdpi.com/2218-273X/12/10/1423 |
| work_keys_str_mv | AT petrabonova identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats AT janakoncekova identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats AT miroslavanemethova identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats AT klaudiapetrova identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats AT martinbona identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats AT miroslavgottlieb identificationofproteinsresponsiblefortheneuroprotectiveeffectofthesecretomederivedfrombloodcellsofremoteischaemicconditionedrats |