Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways
Summary: To improve the treatment of pigmentation disorders, looking for natural and safe inhibitors of melanin synthesis has become an area of research interest. The quinoa husk peptides reportedly elicit various biological activities (e.g., anti-cancer, antioxidant, anti-hypertensive, and so forth...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222019940 |
| _version_ | 1797946186847485952 |
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| author | Caijing Han Bingjie Lin Xinyu Huang Zhaojie Mao Xiaoting Kong Lei Fang Peng Xue Anning Wang Fengxiang Zhang |
| author_facet | Caijing Han Bingjie Lin Xinyu Huang Zhaojie Mao Xiaoting Kong Lei Fang Peng Xue Anning Wang Fengxiang Zhang |
| author_sort | Caijing Han |
| collection | DOAJ |
| description | Summary: To improve the treatment of pigmentation disorders, looking for natural and safe inhibitors of melanin synthesis has become an area of research interest. The quinoa husk peptides reportedly elicit various biological activities (e.g., anti-cancer, antioxidant, anti-hypertensive, and so forth), but its effects on melanin inhibition remain unknown. In the current study, we purified quinoa husk peptides with 30 and 80% ethanol using a macroporous adsorption resin (DA201-C). Component screening revealed that the 80%-ethanol fraction (i.e., QHP fraction) contained numerous short peptides (84.41%) and hydrophobic amino acids (45.60%), while eliciting a superior tyrosinase [TYR]-inhibition rate, 2,2-diphenyl-1-picryhydrazil-scavenging rate, reducing activity, and chelating capacity compared to the 30% fraction and was thus applied in subsequent analyses. Differentially expressed genes in the QHP fraction were primarily enriched in the Akt-signaling pathways based on transcriptomics. Thus, we assessed the expression of related proteins and genes in A375 cells and rat skin cells following treatment with QHP. |
| first_indexed | 2024-04-10T21:06:54Z |
| format | Article |
| id | doaj.art-c739e8b1f175441c996ec70736de0f0d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:54Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c739e8b1f175441c996ec70736de0f0d2023-01-22T04:40:32ZengElsevieriScience2589-00422023-01-01261105721Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathwaysCaijing Han0Bingjie Lin1Xinyu Huang2Zhaojie Mao3Xiaoting Kong4Lei Fang5Peng Xue6Anning Wang7Fengxiang Zhang8School of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, ChinaNeurology Department, The First Affiliated Hospital of Weifang Medical University (Weifang People’s Hospital), Weifang, 261000 Shandong, China; Corresponding authorSchool of Public Health, Weifang Medical University, Weifang, 261053 Shandong, China; Corresponding authorSummary: To improve the treatment of pigmentation disorders, looking for natural and safe inhibitors of melanin synthesis has become an area of research interest. The quinoa husk peptides reportedly elicit various biological activities (e.g., anti-cancer, antioxidant, anti-hypertensive, and so forth), but its effects on melanin inhibition remain unknown. In the current study, we purified quinoa husk peptides with 30 and 80% ethanol using a macroporous adsorption resin (DA201-C). Component screening revealed that the 80%-ethanol fraction (i.e., QHP fraction) contained numerous short peptides (84.41%) and hydrophobic amino acids (45.60%), while eliciting a superior tyrosinase [TYR]-inhibition rate, 2,2-diphenyl-1-picryhydrazil-scavenging rate, reducing activity, and chelating capacity compared to the 30% fraction and was thus applied in subsequent analyses. Differentially expressed genes in the QHP fraction were primarily enriched in the Akt-signaling pathways based on transcriptomics. Thus, we assessed the expression of related proteins and genes in A375 cells and rat skin cells following treatment with QHP.http://www.sciencedirect.com/science/article/pii/S2589004222019940Medical biochemistryplant biochemistrycellular physiology |
| spellingShingle | Caijing Han Bingjie Lin Xinyu Huang Zhaojie Mao Xiaoting Kong Lei Fang Peng Xue Anning Wang Fengxiang Zhang Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways iScience Medical biochemistry plant biochemistry cellular physiology |
| title | Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways |
| title_full | Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways |
| title_fullStr | Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways |
| title_full_unstemmed | Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways |
| title_short | Quinoa husk peptides reduce melanin content via Akt signaling and apoptosis pathways |
| title_sort | quinoa husk peptides reduce melanin content via akt signaling and apoptosis pathways |
| topic | Medical biochemistry plant biochemistry cellular physiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222019940 |
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