Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome
Background Tumor Necrosis Factor Alpha (TNF-α) gene, as an inflammatory factor, plays an important role in reproductive physiology, especially in women with Polycystic Ovary Syndrome (PCOS). Objective This study aims to investigate the relationship between the -1031 T/C polymorphism of TNF-α gene...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
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Qazvin University of Medical Sciences & Health Services
2021-03-01
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| Series: | The Journal of Qazvin University of Medical Sciences |
| Subjects: | |
| Online Access: | https://journal.qums.ac.ir/article-1-3054-en.html |
| _version_ | 1819066874973913088 |
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| author | Mehdi Sahmani Talaate Dabaghi Ghaleh Maryam Yargholi Farshad Foroghi Amir Javadi Khadijeh Taherkhani |
| author_facet | Mehdi Sahmani Talaate Dabaghi Ghaleh Maryam Yargholi Farshad Foroghi Amir Javadi Khadijeh Taherkhani |
| author_sort | Mehdi Sahmani |
| collection | DOAJ |
| description | Background Tumor Necrosis Factor Alpha (TNF-α) gene, as an inflammatory factor, plays an important
role in reproductive physiology, especially in women with Polycystic Ovary Syndrome (PCOS).
Objective This study aims to investigate the relationship between the -1031 T/C polymorphism of TNF-α
gene and biochemical factors in women with PCOS.
Methods In this case-control study, participants were 106 women with PCOS and 114 healthy women
referred to Kosar Hospital in Qazvin, Iran. The TNF-α gene’s polymorphism was determined using Polymerase
Chain Reaction (PCR) technique and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP)
method. Biochemical factors of serum levels were also measured in two groups. Logistic regression analysis
examined the relationship between the frequency of alleles in different states and the risk of PCOS.
Findings There were statistically significant difference in the mean levels of total cholesterol, triglycerides,
testosterone, two-hour blood glucose and body mass index between the groups, whose values were
higher in women with PCOS compared to healthy women (P<0.001). In women with PCOS, the mean serum
levels of triglyceride and high-density lipoprotein were significantly different between the three TT,
CC, TC genotypes of TNF-α gene polymorphism (P<0.05). The results of regression analysis showed that
the TT genotype had significant association with the risk of PCOS (OR=2.43, P=0.006, 95%CI: 1.28-2.62).
Conclusion It seems that there is a relationship between the -1301 (T/C) polymorphism of TNF-α gene
and the risk of PCOS in women. |
| first_indexed | 2024-12-21T16:09:18Z |
| format | Article |
| id | doaj.art-c73b0b8193714e29bf8cf938ab2fd0ac |
| institution | Directory Open Access Journal |
| issn | 1561-3666 2228-7213 |
| language | fas |
| last_indexed | 2024-12-21T16:09:18Z |
| publishDate | 2021-03-01 |
| publisher | Qazvin University of Medical Sciences & Health Services |
| record_format | Article |
| series | The Journal of Qazvin University of Medical Sciences |
| spelling | doaj.art-c73b0b8193714e29bf8cf938ab2fd0ac2022-12-21T18:57:49ZfasQazvin University of Medical Sciences & Health ServicesThe Journal of Qazvin University of Medical Sciences1561-36662228-72132021-03-0124648649710.32598/JQUMS.24.6.1Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary SyndromeMehdi Sahmanihttps://orcid.org/0000-0002-0069-8803Talaate Dabaghi Ghalehhttps://orcid.org/0000-0003-1527-869XMaryam Yargholihttps://orcid.org/0000-0001-9994-4121Farshad Foroghihttps://orcid.org/0000-0001-8553-3604Amir Javadihttps://orcid.org/0000-0001-7715-4583Khadijeh TaherkhaniBackground Tumor Necrosis Factor Alpha (TNF-α) gene, as an inflammatory factor, plays an important role in reproductive physiology, especially in women with Polycystic Ovary Syndrome (PCOS). Objective This study aims to investigate the relationship between the -1031 T/C polymorphism of TNF-α gene and biochemical factors in women with PCOS. Methods In this case-control study, participants were 106 women with PCOS and 114 healthy women referred to Kosar Hospital in Qazvin, Iran. The TNF-α gene’s polymorphism was determined using Polymerase Chain Reaction (PCR) technique and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Biochemical factors of serum levels were also measured in two groups. Logistic regression analysis examined the relationship between the frequency of alleles in different states and the risk of PCOS. Findings There were statistically significant difference in the mean levels of total cholesterol, triglycerides, testosterone, two-hour blood glucose and body mass index between the groups, whose values were higher in women with PCOS compared to healthy women (P<0.001). In women with PCOS, the mean serum levels of triglyceride and high-density lipoprotein were significantly different between the three TT, CC, TC genotypes of TNF-α gene polymorphism (P<0.05). The results of regression analysis showed that the TT genotype had significant association with the risk of PCOS (OR=2.43, P=0.006, 95%CI: 1.28-2.62). Conclusion It seems that there is a relationship between the -1301 (T/C) polymorphism of TNF-α gene and the risk of PCOS in women.https://journal.qums.ac.ir/article-1-3054-en.htmlpolycystic ovary syndrometnf-αgene polymorphism |
| spellingShingle | Mehdi Sahmani Talaate Dabaghi Ghaleh Maryam Yargholi Farshad Foroghi Amir Javadi Khadijeh Taherkhani Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome The Journal of Qazvin University of Medical Sciences polycystic ovary syndrome tnf-α gene polymorphism |
| title | Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome |
| title_full | Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome |
| title_fullStr | Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome |
| title_full_unstemmed | Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome |
| title_short | Association Between the -1031 (T/C) Polymorphism of TNF-α Gene and Biochemical Factors in Women With Polycystic Ovary Syndrome |
| title_sort | association between the 1031 t c polymorphism of tnf α gene and biochemical factors in women with polycystic ovary syndrome |
| topic | polycystic ovary syndrome tnf-α gene polymorphism |
| url | https://journal.qums.ac.ir/article-1-3054-en.html |
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