Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Abstract Bovine enterokinase light chain (EKL) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals. However, recombinant expression in Escherichia coli produces insoluble inclusion bodies, requiring solubilisation, refolding, and a...

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Bibliographic Details
Main Authors:	Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby
Format:	Article
Language:	English
Published:	Nature Portfolio 2022-10-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-022-22574-6

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