Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species
Cryptic species of <i>Aspergillus</i> have rapidly increased in the last few decades. Chronic pulmonary aspergillosis (CPA) is a debilitating fungal infection frequently affecting patients with previous TB. The identification and antifungal susceptibility profiles of different species of...
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MDPI AG
2022-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/4/411 |
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| author | Anna Rozaliyani Asriyani Abdullah Findra Setianingrum Wellyzar Sjamsuridzal Retno Wahyuningsih Anom Bowolaksono Ayu Eka Fatril Robiatul Adawiyah Mulyati Tugiran Ridhawati Syam Heri Wibowo Chris Kosmidis David W. Denning |
| author_facet | Anna Rozaliyani Asriyani Abdullah Findra Setianingrum Wellyzar Sjamsuridzal Retno Wahyuningsih Anom Bowolaksono Ayu Eka Fatril Robiatul Adawiyah Mulyati Tugiran Ridhawati Syam Heri Wibowo Chris Kosmidis David W. Denning |
| author_sort | Anna Rozaliyani |
| collection | DOAJ |
| description | Cryptic species of <i>Aspergillus</i> have rapidly increased in the last few decades. Chronic pulmonary aspergillosis (CPA) is a debilitating fungal infection frequently affecting patients with previous TB. The identification and antifungal susceptibility profiles of different species of <i>Aspergillus</i> are important to support the management of CPA. The aim of this study was to describe the molecular and susceptibility profiles of <i>Aspergillus</i> isolated from CPA patients. The species identity of isolates was determined by combined DNA analyses of internal transcribed space (ITS), partial β-tubulin genes, and part of the calmodulin gene. We revealed a high (27%) prevalence of cryptic species among previous tuberculosis patients with persistent symptoms. Twenty-nine (49%) patients met the criteria for diagnosis of CPA with 24% containing <i>Aspergillus</i> cryptic species. This is the first report of five cryptic <i>Aspergillus</i> species from clinical isolates in Indonesia: <i>A. aculea tus</i>, <i>A. neoniger</i>, <i>A. brunneoviolacues</i>, <i>A. welwitschiae,</i> and <i>A. tubingensis</i>. Significantly, there was decreased sensitivity against itraconazole in the CPA group (66% susceptible to itraconazole) compared to the non-CPA group (90% susceptible to itraconazole) (<i>p</i> = 0.003). The species-level characterisation of <i>Aspergillus</i> and its antifungal susceptibility tests demands greater attention to better the management of CPA patients. |
| first_indexed | 2024-03-09T04:32:17Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:32:17Z |
| publishDate | 2022-04-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-c73faafe7e52473db587430a214964ac2023-12-03T13:34:52ZengMDPI AGJournal of Fungi2309-608X2022-04-018441110.3390/jof8040411Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic SpeciesAnna Rozaliyani0Asriyani Abdullah1Findra Setianingrum2Wellyzar Sjamsuridzal3Retno Wahyuningsih4Anom Bowolaksono5Ayu Eka Fatril6Robiatul Adawiyah7Mulyati Tugiran8Ridhawati Syam9Heri Wibowo10Chris Kosmidis11David W. Denning12Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaMagister Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok 16424, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok 16424, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaManchester Fungal Infection Group, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M23 9LT, UKManchester Fungal Infection Group, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M23 9LT, UKCryptic species of <i>Aspergillus</i> have rapidly increased in the last few decades. Chronic pulmonary aspergillosis (CPA) is a debilitating fungal infection frequently affecting patients with previous TB. The identification and antifungal susceptibility profiles of different species of <i>Aspergillus</i> are important to support the management of CPA. The aim of this study was to describe the molecular and susceptibility profiles of <i>Aspergillus</i> isolated from CPA patients. The species identity of isolates was determined by combined DNA analyses of internal transcribed space (ITS), partial β-tubulin genes, and part of the calmodulin gene. We revealed a high (27%) prevalence of cryptic species among previous tuberculosis patients with persistent symptoms. Twenty-nine (49%) patients met the criteria for diagnosis of CPA with 24% containing <i>Aspergillus</i> cryptic species. This is the first report of five cryptic <i>Aspergillus</i> species from clinical isolates in Indonesia: <i>A. aculea tus</i>, <i>A. neoniger</i>, <i>A. brunneoviolacues</i>, <i>A. welwitschiae,</i> and <i>A. tubingensis</i>. Significantly, there was decreased sensitivity against itraconazole in the CPA group (66% susceptible to itraconazole) compared to the non-CPA group (90% susceptible to itraconazole) (<i>p</i> = 0.003). The species-level characterisation of <i>Aspergillus</i> and its antifungal susceptibility tests demands greater attention to better the management of CPA patients.https://www.mdpi.com/2309-608X/8/4/411<i>Aspergillus</i>crypticantifungal susceptibilitytuberculosischronic pulmonary aspergillosis |
| spellingShingle | Anna Rozaliyani Asriyani Abdullah Findra Setianingrum Wellyzar Sjamsuridzal Retno Wahyuningsih Anom Bowolaksono Ayu Eka Fatril Robiatul Adawiyah Mulyati Tugiran Ridhawati Syam Heri Wibowo Chris Kosmidis David W. Denning Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species Journal of Fungi <i>Aspergillus</i> cryptic antifungal susceptibility tuberculosis chronic pulmonary aspergillosis |
| title | Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species |
| title_full | Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species |
| title_fullStr | Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species |
| title_full_unstemmed | Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species |
| title_short | Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of <i>Aspergillus</i> spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species |
| title_sort | unravelling the molecular identification and antifungal susceptibility profiles of i aspergillus i spp isolated from chronic pulmonary aspergillosis patients in jakarta indonesia the emergence of cryptic species |
| topic | <i>Aspergillus</i> cryptic antifungal susceptibility tuberculosis chronic pulmonary aspergillosis |
| url | https://www.mdpi.com/2309-608X/8/4/411 |
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