Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves
This study aims to assess for the first time the ability of marine polysaccharides and their derivatives to stimulate natural defenses in olive tree leaves. Alginates, ALSM, and ALCM were isolated from the brown algae <i>Sargassum muticum</i> and <i>Cystoseira myriophylloides</i...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/12/17/8842 |
| _version_ | 1827666974678712320 |
|---|---|
| author | Meriem Aitouguinane Zainab El Alaoui-Talibi Halima Rchid Imen Fendri Slim Abdelkafi Mohamed Didi Ould El-Hadj Zakaria Boual Pascal Dubessay Philippe Michaud Mounir Traïkia Guillaume Pierre Cherkaoui El Modafar Cédric Delattre |
| author_facet | Meriem Aitouguinane Zainab El Alaoui-Talibi Halima Rchid Imen Fendri Slim Abdelkafi Mohamed Didi Ould El-Hadj Zakaria Boual Pascal Dubessay Philippe Michaud Mounir Traïkia Guillaume Pierre Cherkaoui El Modafar Cédric Delattre |
| author_sort | Meriem Aitouguinane |
| collection | DOAJ |
| description | This study aims to assess for the first time the ability of marine polysaccharides and their derivatives to stimulate natural defenses in olive tree leaves. Alginates, ALSM, and ALCM were isolated from the brown algae <i>Sargassum muticum</i> and <i>Cystoseira myriophylloides</i>, respectively. The OASM and OACM fractions were obtained after radical depolymerization of ALSM and ALCM, respectively. Three sulfated polysaccharides, arabinogalactans (AGB and AGP) and fucoidans (FUCCM) were extracted from the green alga <i>Codium decorticatum</i> and the brown seaweed <i>C. myriophylloides</i>, respectively. The extraction yield of different extracts was in the range of 0.82–32% and the average molecular weight (M<sub>w</sub>) varied from 3000 Da to 2173 kDa. The M/G ratios of ALSM and ALCM were 0.87 and 1.12, respectively. FUCCM contained 53% of fucose and 12.83% of sulfates. The AGB and AGP fractions were characterized by the presence of a high degree of sulfation and protein (12–23% (<i>w</i>/<i>w</i>)) and were composed mainly of galactose, glucose, and arabinose. The aqueous saccharide solutions were applied to the leaf discs of the olive tree at 0.5 g/L, 1 g/L, and 2 g/L, for 24 h. These molecules triggered defense responses, by showing a differential capacity to induce the activity of the phenylalanine and tyrosine ammonia-lyase (TAL and PAL), polyphenols, and lignin contents in the leaves of the olive tree. Alginates and their derivatives as well as arabinogalactans exhibited an important induction of TAL activity compared to the PAL. The sulfated polysaccharides were more effective compared to the unsulfated polysaccharides (alginates) which were active at a low concentration. The optimum concentration for most of the studied elicitors was 2 g/L. These results suggest the valorization of these molecules derived from marine biomass as inducers of natural defenses of the olive tree to protect against phytopathogens in the context of sustainable development. |
| first_indexed | 2024-03-10T03:01:08Z |
| format | Article |
| id | doaj.art-c741539e3ea14e3aaff32bebfa4b10b3 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:01:08Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-c741539e3ea14e3aaff32bebfa4b10b32023-11-23T12:47:52ZengMDPI AGApplied Sciences2076-34172022-09-011217884210.3390/app12178842Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree LeavesMeriem Aitouguinane0Zainab El Alaoui-Talibi1Halima Rchid2Imen Fendri3Slim Abdelkafi4Mohamed Didi Ould El-Hadj5Zakaria Boual6Pascal Dubessay7Philippe Michaud8Mounir Traïkia9Guillaume Pierre10Cherkaoui El Modafar11Cédric Delattre12Center for Agrobiotechnology and Bioengineering, CNRST Labeled Research Unit (Centre AgroBiotech, URL-CNRST 05), Faculty of Science and Technology, Cadi Ayyad University, Marrakech 40000, MoroccoCenter for Agrobiotechnology and Bioengineering, CNRST Labeled Research Unit (Centre AgroBiotech, URL-CNRST 05), Faculty of Science and Technology, Cadi Ayyad University, Marrakech 40000, MoroccoLaboratory of Biotechnology and Valorization of Plant Resources, Faculty of Sciences, Chouaib Doukkali University, El Jadida 24000, MoroccoLaboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Sciences of Sfax, University of Sfax, Sfax 3038, TunisiaLaboratory of Enzymatic Engineering and Microbiology, Algae Biotechnology Team, National School of Engineers of Sfax, University of Sfax, Sfax 3038, TunisiaLaboratory of Protection of Ecosystems in Arid and Semi-Arid Zones, Faculty of Natural and Life Sciences BP 511, Ouargla Université, Kasdi Merbah University, Ouargla 30000, AlgeriaLaboratory of Protection of Ecosystems in Arid and Semi-Arid Zones, Faculty of Natural and Life Sciences BP 511, Ouargla Université, Kasdi Merbah University, Ouargla 30000, AlgeriaUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, FranceUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, FranceInstitute of Chemistry of Clermont-Ferrand, Clermont Auvergne University, CNRS, SIGMA Clermont, 63000 Clermont-Ferrand, FranceUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, FranceCenter for Agrobiotechnology and Bioengineering, CNRST Labeled Research Unit (Centre AgroBiotech, URL-CNRST 05), Faculty of Science and Technology, Cadi Ayyad University, Marrakech 40000, MoroccoUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, FranceThis study aims to assess for the first time the ability of marine polysaccharides and their derivatives to stimulate natural defenses in olive tree leaves. Alginates, ALSM, and ALCM were isolated from the brown algae <i>Sargassum muticum</i> and <i>Cystoseira myriophylloides</i>, respectively. The OASM and OACM fractions were obtained after radical depolymerization of ALSM and ALCM, respectively. Three sulfated polysaccharides, arabinogalactans (AGB and AGP) and fucoidans (FUCCM) were extracted from the green alga <i>Codium decorticatum</i> and the brown seaweed <i>C. myriophylloides</i>, respectively. The extraction yield of different extracts was in the range of 0.82–32% and the average molecular weight (M<sub>w</sub>) varied from 3000 Da to 2173 kDa. The M/G ratios of ALSM and ALCM were 0.87 and 1.12, respectively. FUCCM contained 53% of fucose and 12.83% of sulfates. The AGB and AGP fractions were characterized by the presence of a high degree of sulfation and protein (12–23% (<i>w</i>/<i>w</i>)) and were composed mainly of galactose, glucose, and arabinose. The aqueous saccharide solutions were applied to the leaf discs of the olive tree at 0.5 g/L, 1 g/L, and 2 g/L, for 24 h. These molecules triggered defense responses, by showing a differential capacity to induce the activity of the phenylalanine and tyrosine ammonia-lyase (TAL and PAL), polyphenols, and lignin contents in the leaves of the olive tree. Alginates and their derivatives as well as arabinogalactans exhibited an important induction of TAL activity compared to the PAL. The sulfated polysaccharides were more effective compared to the unsulfated polysaccharides (alginates) which were active at a low concentration. The optimum concentration for most of the studied elicitors was 2 g/L. These results suggest the valorization of these molecules derived from marine biomass as inducers of natural defenses of the olive tree to protect against phytopathogens in the context of sustainable development.https://www.mdpi.com/2076-3417/12/17/8842olive treeseaweedpolysaccharidesoligosaccharideselicitorsPAL |
| spellingShingle | Meriem Aitouguinane Zainab El Alaoui-Talibi Halima Rchid Imen Fendri Slim Abdelkafi Mohamed Didi Ould El-Hadj Zakaria Boual Pascal Dubessay Philippe Michaud Mounir Traïkia Guillaume Pierre Cherkaoui El Modafar Cédric Delattre Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves Applied Sciences olive tree seaweed polysaccharides oligosaccharides elicitors PAL |
| title | Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves |
| title_full | Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves |
| title_fullStr | Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves |
| title_full_unstemmed | Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves |
| title_short | Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves |
| title_sort | polysaccharides from moroccan green and brown seaweed and their derivatives stimulate natural defenses in olive tree leaves |
| topic | olive tree seaweed polysaccharides oligosaccharides elicitors PAL |
| url | https://www.mdpi.com/2076-3417/12/17/8842 |
| work_keys_str_mv | AT meriemaitouguinane polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT zainabelalaouitalibi polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT halimarchid polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT imenfendri polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT slimabdelkafi polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT mohameddidiouldelhadj polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT zakariaboual polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT pascaldubessay polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT philippemichaud polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT mounirtraikia polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT guillaumepierre polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT cherkaouielmodafar polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves AT cedricdelattre polysaccharidesfrommoroccangreenandbrownseaweedandtheirderivativesstimulatenaturaldefensesinolivetreeleaves |