SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish
The recent development of PAM-less base editors makes it possible to assess the functional impact and pathogenicity of nucleotide mutations in animals. Here the authors show that SpG and SpRY could edit NGN and NNN PAMs in zebrafish using the purified protein with synthetically modified gRNA, respec...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31034-8 |
| _version_ | 1827983730645401600 |
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| author | Fang Liang Yu Zhang Lin Li Yexin Yang Ji-Feng Fei Yanmei Liu Wei Qin |
| author_facet | Fang Liang Yu Zhang Lin Li Yexin Yang Ji-Feng Fei Yanmei Liu Wei Qin |
| author_sort | Fang Liang |
| collection | DOAJ |
| description | The recent development of PAM-less base editors makes it possible to assess the functional impact and pathogenicity of nucleotide mutations in animals. Here the authors show that SpG and SpRY could edit NGN and NNN PAMs in zebrafish using the purified protein with synthetically modified gRNA, respectively. |
| first_indexed | 2024-04-09T22:50:48Z |
| format | Article |
| id | doaj.art-c748bdb7fd474757934c6542326561b8 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:50:48Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c748bdb7fd474757934c6542326561b82023-03-22T11:39:58ZengNature PortfolioNature Communications2041-17232022-06-0113111010.1038/s41467-022-31034-8SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafishFang Liang0Yu Zhang1Lin Li2Yexin Yang3Ji-Feng Fei4Yanmei Liu5Wei Qin6Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal UniversityKey Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal UniversityKey Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal UniversityInstitute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal UniversityDepartment of Pathology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesKey Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal UniversityKey Laboratory of Brain, Cognition and Education Sciences, Ministry of Education; Institute for Brain Research and Rehabilitation, South China Normal UniversityThe recent development of PAM-less base editors makes it possible to assess the functional impact and pathogenicity of nucleotide mutations in animals. Here the authors show that SpG and SpRY could edit NGN and NNN PAMs in zebrafish using the purified protein with synthetically modified gRNA, respectively.https://doi.org/10.1038/s41467-022-31034-8 |
| spellingShingle | Fang Liang Yu Zhang Lin Li Yexin Yang Ji-Feng Fei Yanmei Liu Wei Qin SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish Nature Communications |
| title | SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish |
| title_full | SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish |
| title_fullStr | SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish |
| title_full_unstemmed | SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish |
| title_short | SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish |
| title_sort | spg and spry variants expand the crispr toolbox for genome editing in zebrafish |
| url | https://doi.org/10.1038/s41467-022-31034-8 |
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