Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice
Objective To investigate the characteristics of the macrophage response to transfusion of erythrocytes kept at different storage times in the mouse model of haemorrhagic shock. Methods Erythrocytes were isolated from mice and stored for 7, 21 or 35 days and samples injected intravenously into haemor...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-08-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060520947872 |
| _version_ | 1818838185454600192 |
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| author | Zhen-Zhou Li Xiao-Xiao Wang Li Ma Huan Wang Jia-Ming Xu Xiao-Fang Zhou Yang Liu Jian-Rong Guo |
| author_facet | Zhen-Zhou Li Xiao-Xiao Wang Li Ma Huan Wang Jia-Ming Xu Xiao-Fang Zhou Yang Liu Jian-Rong Guo |
| author_sort | Zhen-Zhou Li |
| collection | DOAJ |
| description | Objective To investigate the characteristics of the macrophage response to transfusion of erythrocytes kept at different storage times in the mouse model of haemorrhagic shock. Methods Erythrocytes were isolated from mice and stored for 7, 21 or 35 days and samples injected intravenously into haemorrhagic shock mice. Changes in macrophages, inflammatory cytokines and T cell differentiation were assessed using flow cytometry or enzyme-linked immunosorbent assay (ELISA). In a second experiment, haemorrhagic shock mice were injected with 21D-erythrocytes and the expression of nuclear factor erythroid 2 p45-related factor 2 (Nrf2), arginine -1 (Arg-1) and inducible nitrous oxide (iNOS) determined. Results The proportion of M1-polarized macrophages was greatest in the 21D group while M2 macrophages tended to increase with the erythrocyte storage time. Levels of inflammatory cytokines and T helper 1 (Th1) cells increased in proportion to erythrocytes storage time. Most regulatory T cells (Treg) were found at 21D. Arg-1 expression was significantly increased in a group that received an heme oxygenase 1 (HO-1) agonist and significantly decreased in a group that received an HO-1 inhibitor but there were no differences in the expression of iNOS or Nrf2. Conclusion 21D storage time may be an important time point for erythrocyte storage and immunity response and Arg-1 may have a role in the macrophage response to erythrocyte infusion. |
| first_indexed | 2024-12-19T03:34:23Z |
| format | Article |
| id | doaj.art-c74ac90e33c240bc9b0946df8f144db2 |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-19T03:34:23Z |
| publishDate | 2020-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-c74ac90e33c240bc9b0946df8f144db22022-12-21T20:37:26ZengSAGE PublishingJournal of International Medical Research1473-23002020-08-014810.1177/0300060520947872Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock miceZhen-Zhou LiXiao-Xiao WangLi MaHuan WangJia-Ming XuXiao-Fang ZhouYang LiuJian-Rong GuoObjective To investigate the characteristics of the macrophage response to transfusion of erythrocytes kept at different storage times in the mouse model of haemorrhagic shock. Methods Erythrocytes were isolated from mice and stored for 7, 21 or 35 days and samples injected intravenously into haemorrhagic shock mice. Changes in macrophages, inflammatory cytokines and T cell differentiation were assessed using flow cytometry or enzyme-linked immunosorbent assay (ELISA). In a second experiment, haemorrhagic shock mice were injected with 21D-erythrocytes and the expression of nuclear factor erythroid 2 p45-related factor 2 (Nrf2), arginine -1 (Arg-1) and inducible nitrous oxide (iNOS) determined. Results The proportion of M1-polarized macrophages was greatest in the 21D group while M2 macrophages tended to increase with the erythrocyte storage time. Levels of inflammatory cytokines and T helper 1 (Th1) cells increased in proportion to erythrocytes storage time. Most regulatory T cells (Treg) were found at 21D. Arg-1 expression was significantly increased in a group that received an heme oxygenase 1 (HO-1) agonist and significantly decreased in a group that received an HO-1 inhibitor but there were no differences in the expression of iNOS or Nrf2. Conclusion 21D storage time may be an important time point for erythrocyte storage and immunity response and Arg-1 may have a role in the macrophage response to erythrocyte infusion.https://doi.org/10.1177/0300060520947872 |
| spellingShingle | Zhen-Zhou Li Xiao-Xiao Wang Li Ma Huan Wang Jia-Ming Xu Xiao-Fang Zhou Yang Liu Jian-Rong Guo Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice Journal of International Medical Research |
| title | Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| title_full | Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| title_fullStr | Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| title_full_unstemmed | Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| title_short | Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| title_sort | influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice |
| url | https://doi.org/10.1177/0300060520947872 |
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