Field study examining the mucosal microbiome in equine glandular gastric disease.
Equine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular muc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295697&type=printable |
| _version_ | 1797377792466223104 |
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| author | Linda J Paul Aaron C Ericsson Frank M Andrews Zachary McAdams Michael L Keowen Michael P St Blanc Heidi E Banse |
| author_facet | Linda J Paul Aaron C Ericsson Frank M Andrews Zachary McAdams Michael L Keowen Michael P St Blanc Heidi E Banse |
| author_sort | Linda J Paul |
| collection | DOAJ |
| description | Equine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. Control biopsies had a higher read count for the phylum Actinomycetota compared to lesion biopsies. Control biopsies also had an enrichment of the genera Staphylococcus and Lawsonella and the species Streptococcus salivarius. Lesion biopsies had an enrichment of the genera Lactobacillus and Actinobacillus and the species Lactobacillus equigenerosi. These results demonstrate differences in the gastric glandular microbiome between sites of disrupted mucosa in horses with EGGD compared to pyloric mucosa of horses without EGGD. Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD. |
| first_indexed | 2024-03-08T19:57:29Z |
| format | Article |
| id | doaj.art-c74b55fc7ab74b13ba0bc692b6054706 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T19:57:29Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c74b55fc7ab74b13ba0bc692b60547062023-12-24T05:33:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011812e029569710.1371/journal.pone.0295697Field study examining the mucosal microbiome in equine glandular gastric disease.Linda J PaulAaron C EricssonFrank M AndrewsZachary McAdamsMichael L KeowenMichael P St BlancHeidi E BanseEquine glandular gastric disease (EGGD) is a common disease among athletic horses that can negatively impact health and performance. The pathophysiology of this EGGD remains poorly understood. Previous studies using controlled populations of horses identified differences in the gastric glandular mucosal microbiome associated with disease. The objective of this study was to compare the gastric microbiome in horses with EGGD and those without across multiple barns and differing management practices. We hypothesized that alterations in the microbiome of the gastric glandular mucosa are associated with EGGD. A secondary objective was to perform a risk factor analysis for EGGD using the diet and management data collected. Microbial populations of biopsies from normal pyloric mucosa of horses without EGGD (control biopsies), normal pyloric mucosa of horses with EGGD (normal biopsies) and areas of glandular mucosal disruption in horses with EGGD (lesion biopsies) were compared. Lesion biopsies had a different microbial community structure than control biopsies. Control biopsies had a higher read count for the phylum Actinomycetota compared to lesion biopsies. Control biopsies also had an enrichment of the genera Staphylococcus and Lawsonella and the species Streptococcus salivarius. Lesion biopsies had an enrichment of the genera Lactobacillus and Actinobacillus and the species Lactobacillus equigenerosi. These results demonstrate differences in the gastric glandular microbiome between sites of disrupted mucosa in horses with EGGD compared to pyloric mucosa of horses without EGGD. Risk factor analysis indicated that exercise duration per week was a risk factor for EGGD.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295697&type=printable |
| spellingShingle | Linda J Paul Aaron C Ericsson Frank M Andrews Zachary McAdams Michael L Keowen Michael P St Blanc Heidi E Banse Field study examining the mucosal microbiome in equine glandular gastric disease. PLoS ONE |
| title | Field study examining the mucosal microbiome in equine glandular gastric disease. |
| title_full | Field study examining the mucosal microbiome in equine glandular gastric disease. |
| title_fullStr | Field study examining the mucosal microbiome in equine glandular gastric disease. |
| title_full_unstemmed | Field study examining the mucosal microbiome in equine glandular gastric disease. |
| title_short | Field study examining the mucosal microbiome in equine glandular gastric disease. |
| title_sort | field study examining the mucosal microbiome in equine glandular gastric disease |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295697&type=printable |
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