Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations]
The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been...
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| Format: | Article |
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F1000 Research Ltd
2016-01-01
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| Online Access: | http://f1000research.com/articles/5-86/v1 |
| _version_ | 1828758295661248512 |
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| author | Jaroslav Michalko Matouš Glanc Catherine Perrot-Rechenmann Jiří Friml |
| author_facet | Jaroslav Michalko Matouš Glanc Catherine Perrot-Rechenmann Jiří Friml |
| author_sort | Jaroslav Michalko |
| collection | DOAJ |
| description | The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants (abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles. |
| first_indexed | 2024-12-11T00:39:46Z |
| format | Article |
| id | doaj.art-c74b653670574eed9b0c25fd44958b66 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-11T00:39:46Z |
| publishDate | 2016-01-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-c74b653670574eed9b0c25fd44958b662022-12-22T01:26:58ZengF1000 Research LtdF1000Research2046-14022016-01-01510.12688/f1000research.7654.18243Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations]Jaroslav Michalko0Matouš Glanc1Catherine Perrot-Rechenmann2Jiří Friml3Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, SlovakiaDepartment of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech RepublicInstitut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay , Versailles, FranceInstitute of Science and Technology Austria, Klosterneuberg, AustriaThe Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants (abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles.http://f1000research.com/articles/5-86/v1GenomicsPlant Genetics & Gene Expression |
| spellingShingle | Jaroslav Michalko Matouš Glanc Catherine Perrot-Rechenmann Jiří Friml Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] F1000Research Genomics Plant Genetics & Gene Expression |
| title | Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] |
| title_full | Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] |
| title_fullStr | Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] |
| title_full_unstemmed | Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] |
| title_short | Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein [version 1; referees: 1 approved, 2 approved with reservations] |
| title_sort | strong morphological defects in conditional arabidopsis abp1 knock down mutants generated in absence of functional abp1 protein version 1 referees 1 approved 2 approved with reservations |
| topic | Genomics Plant Genetics & Gene Expression |
| url | http://f1000research.com/articles/5-86/v1 |
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