Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids
The demands of tissue engineering and regenerative medicine require biomaterials to be accurately deposited into biomimetic shapes, support cellular behaviour and lead to functional tissue formation. Bioinspired yet synthetic biomaterials offer significant advantages over processed, animal-derived p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Materials Today Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259004982200039X |
| _version_ | 1817991521342849024 |
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| author | Kate Firipis Elizabeth Footner Mitchell Boyd-Moss Chaitali Dekiwadia David Nisbet Robert MI. Kapsa Elena Pirogova Richard J. Williams Anita Quigley |
| author_facet | Kate Firipis Elizabeth Footner Mitchell Boyd-Moss Chaitali Dekiwadia David Nisbet Robert MI. Kapsa Elena Pirogova Richard J. Williams Anita Quigley |
| author_sort | Kate Firipis |
| collection | DOAJ |
| description | The demands of tissue engineering and regenerative medicine require biomaterials to be accurately deposited into biomimetic shapes, support cellular behaviour and lead to functional tissue formation. Bioinspired yet synthetic biomaterials offer significant advantages over processed, animal-derived products; including high reproducibility and clinical compliance and specific engineered biomimicry of architecture and biological function. Self-assembling peptides are synthetic highly hydrated scaffolds that are rationally designed to mimic the extracellular matrix of a target tissue. Due to the potential benefits of chemically synthesised self-assembling peptides for clinical translation, their development into tools for biofabrication is warranted. However, these systems can be poorly suited to the demands of biofabrication, particularly when functionalised toward tissue-specific conditions. Here, we demonstrate how to improve biofabrication of self-assembling peptides. The fibrillar network arising from the self-assembling peptide Fmoc-FRGDF (containing cell attachment motif RGD) is combined with the robust polysaccharides agarose and alginate demonstrating enhanced printability and cellular compatibility. This study provides a robust methodology for the on-demand printing of personalised implants with a clinically relevant material. |
| first_indexed | 2024-04-14T01:14:24Z |
| format | Article |
| id | doaj.art-c74d9cdbc3b7426db4a630020c889d0f |
| institution | Directory Open Access Journal |
| issn | 2590-0498 |
| language | English |
| last_indexed | 2024-04-14T01:14:24Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Advances |
| spelling | doaj.art-c74d9cdbc3b7426db4a630020c889d0f2022-12-22T02:20:56ZengElsevierMaterials Today Advances2590-04982022-06-0114100243Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybridsKate Firipis0Elizabeth Footner1Mitchell Boyd-Moss2Chaitali Dekiwadia3David Nisbet4Robert MI. Kapsa5Elena Pirogova6Richard J. Williams7Anita Quigley8Biofab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC, 3000, AustraliaBiofab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC, 3000, AustraliaInstitute of Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Waurn Ponds, VIC, 3216, AustraliaRMIT Microscopy and MicroAnalysis Facility (RMMF), STEM College, RMIT University, Melbourne, VIC, 3000, AustraliaLaboratory of Advanced Biomaterials, The Australian National University, Acton, Canberra, ACT, 2601, Australia; The Graeme Clark Institute, The University of Melbourne, Melbourne, VIC, 3000, Australia; Department of Biomedical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, VIC, 3000, AustraliaBiofab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC, 3000, Australia; Department of Medicine, Melbourne University, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, AustraliaBiofab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC, 3000, AustraliaInstitute of Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Waurn Ponds, VIC, 3216, Australia; Corresponding author.Biofab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC, 3000, Australia; Department of Medicine, Melbourne University, St Vincent's Hospital Melbourne, Fitzroy, VIC, 3065, Australia; Corresponding author.The demands of tissue engineering and regenerative medicine require biomaterials to be accurately deposited into biomimetic shapes, support cellular behaviour and lead to functional tissue formation. Bioinspired yet synthetic biomaterials offer significant advantages over processed, animal-derived products; including high reproducibility and clinical compliance and specific engineered biomimicry of architecture and biological function. Self-assembling peptides are synthetic highly hydrated scaffolds that are rationally designed to mimic the extracellular matrix of a target tissue. Due to the potential benefits of chemically synthesised self-assembling peptides for clinical translation, their development into tools for biofabrication is warranted. However, these systems can be poorly suited to the demands of biofabrication, particularly when functionalised toward tissue-specific conditions. Here, we demonstrate how to improve biofabrication of self-assembling peptides. The fibrillar network arising from the self-assembling peptide Fmoc-FRGDF (containing cell attachment motif RGD) is combined with the robust polysaccharides agarose and alginate demonstrating enhanced printability and cellular compatibility. This study provides a robust methodology for the on-demand printing of personalised implants with a clinically relevant material.http://www.sciencedirect.com/science/article/pii/S259004982200039XSelf-assembling peptidesBioinksBiofabricationPolysaccharideHybrid materials |
| spellingShingle | Kate Firipis Elizabeth Footner Mitchell Boyd-Moss Chaitali Dekiwadia David Nisbet Robert MI. Kapsa Elena Pirogova Richard J. Williams Anita Quigley Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids Materials Today Advances Self-assembling peptides Bioinks Biofabrication Polysaccharide Hybrid materials |
| title | Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids |
| title_full | Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids |
| title_fullStr | Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids |
| title_full_unstemmed | Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids |
| title_short | Biodesigned bioinks for 3D printing via divalent crosslinking of self-assembled peptide-polysaccharide hybrids |
| title_sort | biodesigned bioinks for 3d printing via divalent crosslinking of self assembled peptide polysaccharide hybrids |
| topic | Self-assembling peptides Bioinks Biofabrication Polysaccharide Hybrid materials |
| url | http://www.sciencedirect.com/science/article/pii/S259004982200039X |
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