Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension
Summary: Potato starch suspension (PSS) holds promise as a solution to issues, such as air bubbles and specimen motion, associated with micro-magnetic resonance imaging (micro-MRI) of ex vivo embryos. Here, we present a protocol for using PSS when scanning specimens with micro-MRI. We describe steps...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004501 |
| _version_ | 1797769058730377216 |
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| author | Tomokazu Tsurugizawa Takuma Kumamoto Yoshichika Yoshioka |
| author_facet | Tomokazu Tsurugizawa Takuma Kumamoto Yoshichika Yoshioka |
| author_sort | Tomokazu Tsurugizawa |
| collection | DOAJ |
| description | Summary: Potato starch suspension (PSS) holds promise as a solution to issues, such as air bubbles and specimen motion, associated with micro-magnetic resonance imaging (micro-MRI) of ex vivo embryos. Here, we present a protocol for using PSS when scanning specimens with micro-MRI. We describe steps for preparing samples and potato starch with phosphate-buffered saline. We then detail steps for specimen immersion and micro-MRI scanning. This protocol will enable micro-MRI of not only embryos but also other specimens, such as insects.For complete details on the use and execution of this protocol, please refer to Tsurugizawa et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T21:02:21Z |
| format | Article |
| id | doaj.art-c74ea4f7087140d5a2036440c37a9517 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T21:02:21Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c74ea4f7087140d5a2036440c37a95172023-07-31T04:09:22ZengElsevierSTAR Protocols2666-16672023-09-0143102483Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspensionTomokazu Tsurugizawa0Takuma Kumamoto1Yoshichika Yoshioka2Human Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8568, Japan; Faculty of Engineering, Information and Systems, University of Tsukuba, Tsukuba 305-8573, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan; Center for Information and Neural Networks (CiNet), Osaka University and National Institute of Information and Communications Technology (NICT), Suita 565-0871, Japan; Corresponding authorDevelopmental Neuroscience Project, Department of Brain & Neurosciences, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, JapanGraduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan; Center for Information and Neural Networks (CiNet), Osaka University and National Institute of Information and Communications Technology (NICT), Suita 565-0871, Japan; Corresponding authorSummary: Potato starch suspension (PSS) holds promise as a solution to issues, such as air bubbles and specimen motion, associated with micro-magnetic resonance imaging (micro-MRI) of ex vivo embryos. Here, we present a protocol for using PSS when scanning specimens with micro-MRI. We describe steps for preparing samples and potato starch with phosphate-buffered saline. We then detail steps for specimen immersion and micro-MRI scanning. This protocol will enable micro-MRI of not only embryos but also other specimens, such as insects.For complete details on the use and execution of this protocol, please refer to Tsurugizawa et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004501Developmental BiologyMicrobiologyMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Tomokazu Tsurugizawa Takuma Kumamoto Yoshichika Yoshioka Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension STAR Protocols Developmental Biology Microbiology Microscopy Model Organisms Neuroscience |
| title | Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| title_full | Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| title_fullStr | Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| title_full_unstemmed | Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| title_short | Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| title_sort | micro magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension |
| topic | Developmental Biology Microbiology Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004501 |
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