Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens
<i>Salmonella</i> enterica is an important foodborne pathogen. Commercial poultry are the main reservoirs of <i>Salmonella</i> enterica, leading to the contamination of food and outbreaks in humans. The vaccination of chickens is one of the most important strategies to reduce...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/2/226 |
| _version_ | 1797476149169750016 |
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| author | Leonardo Sáenz Miguel Guzmán Sonia Vidal Mario Caruffo Daniela Siel Caridad Zayas Rodolfo Paredes Carolina Valenzuela Héctor Hidalgo Oliver Pérez Lisette Lapierre |
| author_facet | Leonardo Sáenz Miguel Guzmán Sonia Vidal Mario Caruffo Daniela Siel Caridad Zayas Rodolfo Paredes Carolina Valenzuela Héctor Hidalgo Oliver Pérez Lisette Lapierre |
| author_sort | Leonardo Sáenz |
| collection | DOAJ |
| description | <i>Salmonella</i> enterica is an important foodborne pathogen. Commercial poultry are the main reservoirs of <i>Salmonella</i> enterica, leading to the contamination of food and outbreaks in humans. The vaccination of chickens is one of the most important strategies to reduce the number of <i>Salmonella</i> in poultry farms. Unfortunately, commercial vaccines have not been fully effective in controlling the spread and do not contain all the <i>Salmonella</i> serovars that circulate on farms. In this study, we evaluate a new, cochleate-based, trivalent injectable vaccine against <i>S.</i> Enteritidis, <i>S.</i> Typhimurium and <i>S.</i> Infantis, describing the vaccine security, capacity to induce specific anti-<i>Salmonella</i> serovar IgY and the gene expression of immune markers related to CD4 and CD8 T-cell-mediated immunity. Efficacy was evaluated through oral challenges performed separately for each <i>Salmonella</i> serotype. The efficacy and safety of the trivalent vaccine was proven under controlled conditions. The vaccine has no local or systemic reactions or adverse effects on poultry performance related to the vaccine. The vaccine provided significantly increased serum IgY titer levels, significantly reduced <i>Salmonella</i> CFU/g present in the cecum and an increased CD4+/CD8+ ratio in vaccinated animals when challenged with <i>S.</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium. These results indicate that this new trivalent vaccine does not generate adverse effects in poultry and produces an increase in neutralizing antibodies against the three <i>Salmonella</i> serovars. |
| first_indexed | 2024-03-09T20:54:50Z |
| format | Article |
| id | doaj.art-c750c5b6b2f44ef281ca6d744bfabe3c |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T20:54:50Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-c750c5b6b2f44ef281ca6d744bfabe3c2023-11-23T22:25:33ZengMDPI AGVaccines2076-393X2022-02-0110222610.3390/vaccines10020226Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying HensLeonardo Sáenz0Miguel Guzmán1Sonia Vidal2Mario Caruffo3Daniela Siel4Caridad Zayas5Rodolfo Paredes6Carolina Valenzuela7Héctor Hidalgo8Oliver Pérez9Lisette Lapierre10Faculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileEscuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370035, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileInstituto de Ciencias Básicas Y Preclínicas “Victoria de Girón”, Universidad de Ciencias Médicas de La Habana, Havana 10600, CubaFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, Chile<i>Salmonella</i> enterica is an important foodborne pathogen. Commercial poultry are the main reservoirs of <i>Salmonella</i> enterica, leading to the contamination of food and outbreaks in humans. The vaccination of chickens is one of the most important strategies to reduce the number of <i>Salmonella</i> in poultry farms. Unfortunately, commercial vaccines have not been fully effective in controlling the spread and do not contain all the <i>Salmonella</i> serovars that circulate on farms. In this study, we evaluate a new, cochleate-based, trivalent injectable vaccine against <i>S.</i> Enteritidis, <i>S.</i> Typhimurium and <i>S.</i> Infantis, describing the vaccine security, capacity to induce specific anti-<i>Salmonella</i> serovar IgY and the gene expression of immune markers related to CD4 and CD8 T-cell-mediated immunity. Efficacy was evaluated through oral challenges performed separately for each <i>Salmonella</i> serotype. The efficacy and safety of the trivalent vaccine was proven under controlled conditions. The vaccine has no local or systemic reactions or adverse effects on poultry performance related to the vaccine. The vaccine provided significantly increased serum IgY titer levels, significantly reduced <i>Salmonella</i> CFU/g present in the cecum and an increased CD4+/CD8+ ratio in vaccinated animals when challenged with <i>S.</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium. These results indicate that this new trivalent vaccine does not generate adverse effects in poultry and produces an increase in neutralizing antibodies against the three <i>Salmonella</i> serovars.https://www.mdpi.com/2076-393X/10/2/226<i>Salmonella</i> InfantisvaccinecochleateELISApoultry |
| spellingShingle | Leonardo Sáenz Miguel Guzmán Sonia Vidal Mario Caruffo Daniela Siel Caridad Zayas Rodolfo Paredes Carolina Valenzuela Héctor Hidalgo Oliver Pérez Lisette Lapierre Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens Vaccines <i>Salmonella</i> Infantis vaccine cochleate ELISA poultry |
| title | Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens |
| title_full | Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens |
| title_fullStr | Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens |
| title_full_unstemmed | Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens |
| title_short | Efficacy of Multivalent, Cochleate-Based Vaccine against <i>Salmonella</i> Infantis, <i>S.</i> Enteritidis and <i>S.</i> Typhimurium in Laying Hens |
| title_sort | efficacy of multivalent cochleate based vaccine against i salmonella i infantis i s i enteritidis and i s i typhimurium in laying hens |
| topic | <i>Salmonella</i> Infantis vaccine cochleate ELISA poultry |
| url | https://www.mdpi.com/2076-393X/10/2/226 |
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