Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital
Introduction. There is no consensus on the management of patients with recurrent uncomplicated lower urinary tract infection (uLUTI), which makes it difficult to carry out effective therapy.Purpose of the study. To study the microbial patterns of urine and uropathogens' antibiotic resistance in...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation
2020-10-01
|
| Series: | Vestnik Urologii |
| Subjects: | |
| Online Access: | https://www.urovest.ru/jour/article/view/360 |
| _version_ | 1797272174969487360 |
|---|---|
| author | Yu. L. Naboka A. K. Alkina M. I. Kogan I. A. Gudima Kh. S. Ibishev K. T. Jalagoniya M. L. Chernitskaya |
| author_facet | Yu. L. Naboka A. K. Alkina M. I. Kogan I. A. Gudima Kh. S. Ibishev K. T. Jalagoniya M. L. Chernitskaya |
| author_sort | Yu. L. Naboka |
| collection | DOAJ |
| description | Introduction. There is no consensus on the management of patients with recurrent uncomplicated lower urinary tract infection (uLUTI), which makes it difficult to carry out effective therapy.Purpose of the study. To study the microbial patterns of urine and uropathogens' antibiotic resistance in a urological hospital from 2010 to 2017.Materials and methods. The results of bacteriological studies and the data of individual antibiotic susceptibility testing of patients with recurrent uLUTI (n = 502) were retrospectively analyzed. Inclusion criteria of the study: consent of patients to participate in the study, the presence of clinical manifestations of recurrent uLUTI in anamnesis, two episodes within six months or three during the year, leukocyturia in the urinalysis, the absence of sexually transmitted diseases at the time of the study and in anamnesis and also vaginal discharge. Bacteriological studies of the midstream morning urine sample before the prescription of antibiotic therapy were carried out with the determination of antibiotic sensitivity/resistance and production of extended-spectrum p-lactamases. In addition to the standard set of culture media, chromogenic media, aerobic and anaerobic culturing conditions were used. Statistical analysis was performed in the statistical processing and data visualization environment «R ver 3.2» («R Foundation for Statistical Computing», Vienna, Austria).Results. During the 8-year monitoring of the microbiota, the urine of patients with recurrent uLUTI was characterized by a microbial pattern certain constancy, but with the predominance (94.1% - 99.1%) of anaerobic-aerobic associations. The antibiotic resistance of most causative and debatable uropathogens increased, and the detection frequency of enterobacteria producing extended-spectrum p-lactamases also increased. Significant differences in the average levels of bacteriuria in the study period for most taxa of microorganisms were not detected.Conclusion. The detection frequencies of E. coli vary around 50.0%, for other causative pathogens a lower detection rate is noted, while non-clostridial anaerobic bacteria are excreted in 96.6% of cases in patients with recurrent uLUTI. The antibiotic resistance of causative and debatable pathogens and the detection frequencies of enterobacteria producing extended-spectrum p-lactamases are increasing, which necessitates a revision of the etiological structure and approaches to empirical therapy of recurrent uLUTI. |
| first_indexed | 2024-03-07T14:24:37Z |
| format | Article |
| id | doaj.art-c753d2b5e170414f989d7d7b7bc4451d |
| institution | Directory Open Access Journal |
| issn | 2308-6424 |
| language | Russian |
| last_indexed | 2024-03-07T14:24:37Z |
| publishDate | 2020-10-01 |
| publisher | State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation |
| record_format | Article |
| series | Vestnik Urologii |
| spelling | doaj.art-c753d2b5e170414f989d7d7b7bc4451d2024-03-06T08:56:04ZrusState Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian FederationVestnik Urologii2308-64242020-10-0183475710.21886/2308-6424-2020-8-3-47-57258Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospitalYu. L. Naboka0A. K. Alkina1M. I. Kogan2I. A. Gudima3Kh. S. Ibishev4K. T. Jalagoniya5M. L. Chernitskaya6Rostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityIntroduction. There is no consensus on the management of patients with recurrent uncomplicated lower urinary tract infection (uLUTI), which makes it difficult to carry out effective therapy.Purpose of the study. To study the microbial patterns of urine and uropathogens' antibiotic resistance in a urological hospital from 2010 to 2017.Materials and methods. The results of bacteriological studies and the data of individual antibiotic susceptibility testing of patients with recurrent uLUTI (n = 502) were retrospectively analyzed. Inclusion criteria of the study: consent of patients to participate in the study, the presence of clinical manifestations of recurrent uLUTI in anamnesis, two episodes within six months or three during the year, leukocyturia in the urinalysis, the absence of sexually transmitted diseases at the time of the study and in anamnesis and also vaginal discharge. Bacteriological studies of the midstream morning urine sample before the prescription of antibiotic therapy were carried out with the determination of antibiotic sensitivity/resistance and production of extended-spectrum p-lactamases. In addition to the standard set of culture media, chromogenic media, aerobic and anaerobic culturing conditions were used. Statistical analysis was performed in the statistical processing and data visualization environment «R ver 3.2» («R Foundation for Statistical Computing», Vienna, Austria).Results. During the 8-year monitoring of the microbiota, the urine of patients with recurrent uLUTI was characterized by a microbial pattern certain constancy, but with the predominance (94.1% - 99.1%) of anaerobic-aerobic associations. The antibiotic resistance of most causative and debatable uropathogens increased, and the detection frequency of enterobacteria producing extended-spectrum p-lactamases also increased. Significant differences in the average levels of bacteriuria in the study period for most taxa of microorganisms were not detected.Conclusion. The detection frequencies of E. coli vary around 50.0%, for other causative pathogens a lower detection rate is noted, while non-clostridial anaerobic bacteria are excreted in 96.6% of cases in patients with recurrent uLUTI. The antibiotic resistance of causative and debatable pathogens and the detection frequencies of enterobacteria producing extended-spectrum p-lactamases are increasing, which necessitates a revision of the etiological structure and approaches to empirical therapy of recurrent uLUTI.https://www.urovest.ru/jour/article/view/360urine microbiotaantibiotic resistancelower urinary tract infections |
| spellingShingle | Yu. L. Naboka A. K. Alkina M. I. Kogan I. A. Gudima Kh. S. Ibishev K. T. Jalagoniya M. L. Chernitskaya Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital Vestnik Urologii urine microbiota antibiotic resistance lower urinary tract infections |
| title | Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital |
| title_full | Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital |
| title_fullStr | Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital |
| title_full_unstemmed | Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital |
| title_short | Monitoring of urinary microbiota and uropathogens' antibiotic resistance in one urological hospital |
| title_sort | monitoring of urinary microbiota and uropathogens antibiotic resistance in one urological hospital |
| topic | urine microbiota antibiotic resistance lower urinary tract infections |
| url | https://www.urovest.ru/jour/article/view/360 |
| work_keys_str_mv | AT yulnaboka monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT akalkina monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT mikogan monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT iagudima monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT khsibishev monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT ktjalagoniya monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital AT mlchernitskaya monitoringofurinarymicrobiotaanduropathogensantibioticresistanceinoneurologicalhospital |