Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis
Quantification of plasma cell-free Epstein Barr virus DNA (cf EBV DNA) has been suggested as a promising liquid biopsy assay for screening and early detection of nasopharyngeal carcinoma (NPC). However, the diagnostic value of this assay is currently not known in the population of Vietnam, one of th...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-07-01
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| Series: | Cancer Control |
| Online Access: | https://doi.org/10.1177/1073274820944286 |
| _version_ | 1818841350456475648 |
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| author | Vu Nguyen Quynh Anh BA Nguyen Van Ba MD, PhD Do Tram Anh MD Nguyen Dinh Ung MD Nguyen Hoang Hiep PhD Vu Thi Ly BA Dinh Thi Thu Hang PhD Bui Tien Sy MD, PhD Hoang Dao Chinh MD Le Minh Ky MD, PhD Vu Truong Phong MD, PhD Nguyen Kim Luu MD, PhD Nguyen Thanh Trung BA Ho Anh Son MD, PhD Hoang Van Luong MD, PhD Nghiem Duc Thuan MD, PhD Ngo Thanh Tung MD, PhD Ho Huu Tho MD, PhD |
| author_facet | Vu Nguyen Quynh Anh BA Nguyen Van Ba MD, PhD Do Tram Anh MD Nguyen Dinh Ung MD Nguyen Hoang Hiep PhD Vu Thi Ly BA Dinh Thi Thu Hang PhD Bui Tien Sy MD, PhD Hoang Dao Chinh MD Le Minh Ky MD, PhD Vu Truong Phong MD, PhD Nguyen Kim Luu MD, PhD Nguyen Thanh Trung BA Ho Anh Son MD, PhD Hoang Van Luong MD, PhD Nghiem Duc Thuan MD, PhD Ngo Thanh Tung MD, PhD Ho Huu Tho MD, PhD |
| author_sort | Vu Nguyen Quynh Anh BA |
| collection | DOAJ |
| description | Quantification of plasma cell-free Epstein Barr virus DNA (cf EBV DNA) has been suggested as a promising liquid biopsy assay for screening and early detection of nasopharyngeal carcinoma (NPC). However, the diagnostic value of this assay is currently not known in the population of Vietnam, one of the countries which contributed the most to the NPC cases. Herein, we have reported a highly sensitive quantitative polymerase chain reaction (qPCR)-based assay targeting cf EBV DNA for the detection of NPC. A standard curve with linear regression, R 2 = 0.9961 (range: 25-150 000 copies/mL) and a detection limit of 25 copies/mL were obtained using an EBV standard panel provided by the Chinese University of Hong Kong. The clinical performance of this assay was assessed using plasma samples obtained from 261 Vietnamese individuals. The optimized qPCR assay detected cf EBV DNA in plasma with a sensitivity of 97.4% and a specificity of 98.2%. The absolute quantitative results of pretreatment cf EBV DNA and patient overall clinical stages were statistically correlated ( P < .05). In summary, the remarkably high sensitivity and specificity of our optimized qPCR assay strongly supports the wide use of cf EBV DNA quantification as a routine noninvasive method in early diagnosis and management of patients with NPC. |
| first_indexed | 2024-12-19T04:24:41Z |
| format | Article |
| id | doaj.art-c7553214cd72426488d49edf25339a20 |
| institution | Directory Open Access Journal |
| issn | 1073-2748 |
| language | English |
| last_indexed | 2024-12-19T04:24:41Z |
| publishDate | 2020-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cancer Control |
| spelling | doaj.art-c7553214cd72426488d49edf25339a202022-12-21T20:36:04ZengSAGE PublishingCancer Control1073-27482020-07-012710.1177/1073274820944286Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma DiagnosisVu Nguyen Quynh Anh BA0Nguyen Van Ba MD, PhD1Do Tram Anh MD2Nguyen Dinh Ung MD3Nguyen Hoang Hiep PhD4Vu Thi Ly BA5Dinh Thi Thu Hang PhD6Bui Tien Sy MD, PhD7Hoang Dao Chinh MD8Le Minh Ky MD, PhD9Vu Truong Phong MD, PhD10Nguyen Kim Luu MD, PhD11Nguyen Thanh Trung BA12Ho Anh Son MD, PhD13Hoang Van Luong MD, PhD14Nghiem Duc Thuan MD, PhD15Ngo Thanh Tung MD, PhD16Ho Huu Tho MD, PhD17 Department of Genomics and Cytogenetics, Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Oncology Centre, 103 Military Hospital, Vietnam Military Medical University, Hanoi, Vietnam ENT Department, 103 Military Hospital, Vietnam Military Medical University, Hanoi, Vietnam Department of Genomics and Cytogenetics, Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Department of Genomics and Cytogenetics, Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Department of Genomics and Cytogenetics, Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Department of Microbiology, 108 Military Central Hospital, Hanoi, Vietnam Department of Oncology and Radiation, 108 Military Central Hospital, Hanoi, Vietnam Oncology and Head & Neck Surgery Centre, Vietnam National ENT Hospital, Hanoi, Vietnam Oncology and Head & Neck Surgery Centre, Vietnam National ENT Hospital, Hanoi, Vietnam Department of Radiation, 103 Military Hospital, Vietnam Military Medical University, Hanoi, Vietnam HUS High School for Gifted Students, Hanoi, Vietnam Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam ENT Department, 103 Military Hospital, Vietnam Military Medical University, Hanoi, Vietnam Department of Head & Neck Cancer and Radiation Oncology, Vietnam National K Hospital, Hanoi, Vietnam Department of Genomics and Cytogenetics, Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, VietnamQuantification of plasma cell-free Epstein Barr virus DNA (cf EBV DNA) has been suggested as a promising liquid biopsy assay for screening and early detection of nasopharyngeal carcinoma (NPC). However, the diagnostic value of this assay is currently not known in the population of Vietnam, one of the countries which contributed the most to the NPC cases. Herein, we have reported a highly sensitive quantitative polymerase chain reaction (qPCR)-based assay targeting cf EBV DNA for the detection of NPC. A standard curve with linear regression, R 2 = 0.9961 (range: 25-150 000 copies/mL) and a detection limit of 25 copies/mL were obtained using an EBV standard panel provided by the Chinese University of Hong Kong. The clinical performance of this assay was assessed using plasma samples obtained from 261 Vietnamese individuals. The optimized qPCR assay detected cf EBV DNA in plasma with a sensitivity of 97.4% and a specificity of 98.2%. The absolute quantitative results of pretreatment cf EBV DNA and patient overall clinical stages were statistically correlated ( P < .05). In summary, the remarkably high sensitivity and specificity of our optimized qPCR assay strongly supports the wide use of cf EBV DNA quantification as a routine noninvasive method in early diagnosis and management of patients with NPC.https://doi.org/10.1177/1073274820944286 |
| spellingShingle | Vu Nguyen Quynh Anh BA Nguyen Van Ba MD, PhD Do Tram Anh MD Nguyen Dinh Ung MD Nguyen Hoang Hiep PhD Vu Thi Ly BA Dinh Thi Thu Hang PhD Bui Tien Sy MD, PhD Hoang Dao Chinh MD Le Minh Ky MD, PhD Vu Truong Phong MD, PhD Nguyen Kim Luu MD, PhD Nguyen Thanh Trung BA Ho Anh Son MD, PhD Hoang Van Luong MD, PhD Nghiem Duc Thuan MD, PhD Ngo Thanh Tung MD, PhD Ho Huu Tho MD, PhD Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis Cancer Control |
| title | Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis |
| title_full | Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis |
| title_fullStr | Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis |
| title_full_unstemmed | Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis |
| title_short | Validation of a Highly Sensitive qPCR Assay for the Detection of Plasma Cell-Free Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma Diagnosis |
| title_sort | validation of a highly sensitive qpcr assay for the detection of plasma cell free epstein barr virus dna in nasopharyngeal carcinoma diagnosis |
| url | https://doi.org/10.1177/1073274820944286 |
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