A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease
Background: Alzheimer’s disease (AD) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. Thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of AD. Objective: The study aims to develop a bio...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2021-04-01
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| Series: | Journal of Biomedical Physics and Engineering |
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| Online Access: | https://jbpe.sums.ac.ir/article_47375_d78e362360728230db4ad66839e0c3f7.pdf |
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| author | Rezvan Dehdari Vais Hossein Yadegari Hossein Heli Naghmeh Sattarahmady |
| author_facet | Rezvan Dehdari Vais Hossein Yadegari Hossein Heli Naghmeh Sattarahmady |
| author_sort | Rezvan Dehdari Vais |
| collection | DOAJ |
| description | Background: Alzheimer’s disease (AD) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. Thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of AD. Objective: The study aims to develop a biosensor based on a molecularly imprinted poly-pyrrole for detection of β-amyloid.Material and Methods: In this experimental study, an imprinted poly-pyrrole was employed as an artificial receptor synthesized by electro-polymerization of pyrrole on screen-printed carbon electrodes in the presence of β-amyloid. β-amyloid acts as a molecular template within the polymer. The biosensor was evaluated by cyclic voltammetry using ferro/ferricyanide marker. The parameters influencing the biosensor performance, including electro-polymerization cycle umbers and β-amyloid binding time were optimized to achieve the best biosensor sensitivity. Results: The β-amyloid binding affinity with the biosensor surface was evaluated by the Freundlich isotherm, and Freundlich constant and exponent were obtained as 0.22 ng mL-1 and 10.60, respectively. The biosensor demonstrated a detection limit of 1.2 pg mL-1. The biosensor was applied for β-amyloid determination in artificial cerebrospinal fluid. Conclusion: The biosensor is applicable for early Alzheimer’s disease detection. |
| first_indexed | 2024-12-14T20:54:24Z |
| format | Article |
| id | doaj.art-c75727dda11a4ad3a8d689ad70424918 |
| institution | Directory Open Access Journal |
| issn | 2251-7200 2251-7200 |
| language | English |
| last_indexed | 2024-12-14T20:54:24Z |
| publishDate | 2021-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj.art-c75727dda11a4ad3a8d689ad704249182022-12-21T22:47:43ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002251-72002021-04-0111221522810.31661/jbpe.v0i0.107047375A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s DiseaseRezvan Dehdari Vais0Hossein Yadegari1Hossein Heli2Naghmeh Sattarahmady3PhD Candidate, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranPhD, Department of Mechanical and Materials Engineering, University of Western Ontario, London, Ontario N6A 5B9, CanadaPhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranPhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBackground: Alzheimer’s disease (AD) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. Thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of AD. Objective: The study aims to develop a biosensor based on a molecularly imprinted poly-pyrrole for detection of β-amyloid.Material and Methods: In this experimental study, an imprinted poly-pyrrole was employed as an artificial receptor synthesized by electro-polymerization of pyrrole on screen-printed carbon electrodes in the presence of β-amyloid. β-amyloid acts as a molecular template within the polymer. The biosensor was evaluated by cyclic voltammetry using ferro/ferricyanide marker. The parameters influencing the biosensor performance, including electro-polymerization cycle umbers and β-amyloid binding time were optimized to achieve the best biosensor sensitivity. Results: The β-amyloid binding affinity with the biosensor surface was evaluated by the Freundlich isotherm, and Freundlich constant and exponent were obtained as 0.22 ng mL-1 and 10.60, respectively. The biosensor demonstrated a detection limit of 1.2 pg mL-1. The biosensor was applied for β-amyloid determination in artificial cerebrospinal fluid. Conclusion: The biosensor is applicable for early Alzheimer’s disease detection.https://jbpe.sums.ac.ir/article_47375_d78e362360728230db4ad66839e0c3f7.pdfbeta-amyloidβ-amyloid (1-40)molecular imprintingartificial antibodyneurodegenerative diseasesensory aidsbiosensing techniquebioprobe |
| spellingShingle | Rezvan Dehdari Vais Hossein Yadegari Hossein Heli Naghmeh Sattarahmady A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease Journal of Biomedical Physics and Engineering beta-amyloid β-amyloid (1-40) molecular imprinting artificial antibody neurodegenerative disease sensory aids biosensing technique bioprobe |
| title | A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease |
| title_full | A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease |
| title_fullStr | A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease |
| title_full_unstemmed | A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease |
| title_short | A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease |
| title_sort | β amyloid 1 42 biosensor based on molecularly imprinted poly pyrrole for early diagnosis of alzheimer s disease |
| topic | beta-amyloid β-amyloid (1-40) molecular imprinting artificial antibody neurodegenerative disease sensory aids biosensing technique bioprobe |
| url | https://jbpe.sums.ac.ir/article_47375_d78e362360728230db4ad66839e0c3f7.pdf |
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