Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis
ObjectiveTo establish a rat model of collagen-induced arthritis (CIA), a rat model of rheumatoid arthritis, and to study the protective effect of dexamethasone (DEX) on cartilage in CIA rats.MethodsThirty Wistar rats were randomly divided into three groups: normal, model, and DEX groups. The CIA mod...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2022-10-01
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| Series: | Shiyan dongwu yu bijiao yixue |
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| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.066 |
| _version_ | 1811179924318322688 |
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| author | XU Bingxin FAN Kaijian WANG Tingyu CHEN Huijin |
| author_facet | XU Bingxin FAN Kaijian WANG Tingyu CHEN Huijin |
| author_sort | XU Bingxin |
| collection | DOAJ |
| description | ObjectiveTo establish a rat model of collagen-induced arthritis (CIA), a rat model of rheumatoid arthritis, and to study the protective effect of dexamethasone (DEX) on cartilage in CIA rats.MethodsThirty Wistar rats were randomly divided into three groups: normal, model, and DEX groups. The CIA model was established by intradermal injection of a white emulsion mixed with 0.1 mL bovine type II collagen and Freund's incomplete adjuvant in equal proportions into the distal part of the rat tail. The dates of the two injections were recorded as days 0 and 7, respectively. After the establishment of the CIA model, DEX (1 mg/kg) was intraperitoneally injected in the treatment group 3 times a week for 4 weeks. After the administration, the rats were euthanized, and the knee cartilages of 10 rats in each group were divided into three parts. The first part was fixed, decalcified, and stained with Alcian blue to observe the pathological changes in the cartilage layer. The second part was used for detection of mRNA expression of matrix metalloproteinase (MMP)-9 and MMP-13, and a disintegrin-like and metalloproteinase with thrombo-spondin motifs (ADAMTS)-4 and ADAMTS-5 by using real-time quantitative PCR analysis in the femoral head cartilage. The third part was used for detection of the expression levels of MMP-9, MMP-13, and ADAMTS-5 using immunohistochemistry in the cartilage layer of knee joint.ResultsCompared with the model group, the decrease of cartilage area, cartilage thickness, and cartilage cell numbers were significantly inhibited after DEX treatment (P<0.05). The cartilage surface was smooth and the invasion degree significantly improved, which indicated that cartilage destruction was inhibited. After DEX treatment, the mRNA levels of MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 in the cartilage of CIA rats were decreased (P<0.001), and the protein expression levels of MMP-9, MMP-13, and ADAMTS-5 were also significantly decreased (P<0.05).ConclusionDEX can inhibit the expression of various MMP in CIA rats and has a therapeutic effect on cartilage destruction. |
| first_indexed | 2024-04-11T06:41:51Z |
| format | Article |
| id | doaj.art-c7572d0ba21340acb909554afa1063ce |
| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-04-11T06:41:51Z |
| publishDate | 2022-10-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-c7572d0ba21340acb909554afa1063ce2022-12-22T04:39:30ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172022-10-0142541642210.12300/j.issn.1674-5817.2022.0661674-5817（2022）05-0416-07Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced ArthritisXU Bingxin0FAN Kaijian1WANG Tingyu2CHEN Huijin3Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaObjectiveTo establish a rat model of collagen-induced arthritis (CIA), a rat model of rheumatoid arthritis, and to study the protective effect of dexamethasone (DEX) on cartilage in CIA rats.MethodsThirty Wistar rats were randomly divided into three groups: normal, model, and DEX groups. The CIA model was established by intradermal injection of a white emulsion mixed with 0.1 mL bovine type II collagen and Freund's incomplete adjuvant in equal proportions into the distal part of the rat tail. The dates of the two injections were recorded as days 0 and 7, respectively. After the establishment of the CIA model, DEX (1 mg/kg) was intraperitoneally injected in the treatment group 3 times a week for 4 weeks. After the administration, the rats were euthanized, and the knee cartilages of 10 rats in each group were divided into three parts. The first part was fixed, decalcified, and stained with Alcian blue to observe the pathological changes in the cartilage layer. The second part was used for detection of mRNA expression of matrix metalloproteinase (MMP)-9 and MMP-13, and a disintegrin-like and metalloproteinase with thrombo-spondin motifs (ADAMTS)-4 and ADAMTS-5 by using real-time quantitative PCR analysis in the femoral head cartilage. The third part was used for detection of the expression levels of MMP-9, MMP-13, and ADAMTS-5 using immunohistochemistry in the cartilage layer of knee joint.ResultsCompared with the model group, the decrease of cartilage area, cartilage thickness, and cartilage cell numbers were significantly inhibited after DEX treatment (P<0.05). The cartilage surface was smooth and the invasion degree significantly improved, which indicated that cartilage destruction was inhibited. After DEX treatment, the mRNA levels of MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 in the cartilage of CIA rats were decreased (P<0.001), and the protein expression levels of MMP-9, MMP-13, and ADAMTS-5 were also significantly decreased (P<0.05).ConclusionDEX can inhibit the expression of various MMP in CIA rats and has a therapeutic effect on cartilage destruction.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.066rheumatoid arthritiscollagen induced arthritisdexamethasonecartilagewistar rats |
| spellingShingle | XU Bingxin FAN Kaijian WANG Tingyu CHEN Huijin Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis Shiyan dongwu yu bijiao yixue rheumatoid arthritis collagen induced arthritis dexamethasone cartilage wistar rats |
| title | Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis |
| title_full | Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis |
| title_fullStr | Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis |
| title_full_unstemmed | Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis |
| title_short | Effect of Dexamethasone on Cartilage Degeneration in Rats with Collagen-induced Arthritis |
| title_sort | effect of dexamethasone on cartilage degeneration in rats with collagen induced arthritis |
| topic | rheumatoid arthritis collagen induced arthritis dexamethasone cartilage wistar rats |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2022.066 |
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