The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota
The preterm infant gut microbiota is influenced by environmental, endogenous, maternal, and genetic factors. Although siblings share similar gut microbial composition, it is not known how genetic relatedness affects alpha diversity and specific taxa abundances in preterm infants. We analyzed the 16S...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/278 |
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| author | Shen Jean Lim Miriam Aguilar-Lopez Christine Wetzel Samia V. O. Dutra Vanessa Bray Maureen W. Groer Sharon M. Donovan Thao Ho |
| author_facet | Shen Jean Lim Miriam Aguilar-Lopez Christine Wetzel Samia V. O. Dutra Vanessa Bray Maureen W. Groer Sharon M. Donovan Thao Ho |
| author_sort | Shen Jean Lim |
| collection | DOAJ |
| description | The preterm infant gut microbiota is influenced by environmental, endogenous, maternal, and genetic factors. Although siblings share similar gut microbial composition, it is not known how genetic relatedness affects alpha diversity and specific taxa abundances in preterm infants. We analyzed the 16S rRNA gene content of stool samples, ≤ and >3 weeks postnatal age, and clinical data from preterm multiplets and singletons at two Neonatal Intensive Care Units (NICUs), Tampa General Hospital (TGH; FL, USA) and Carle Hospital (IL, USA). Weeks on bovine milk-based fortifier (BMF) and weight gain velocity were significant predictors of alpha diversity. Alpha diversity between siblings were significantly correlated, particularly at ≤3 weeks postnatal age and in the TGH NICU, after controlling for clinical factors. Siblings shared higher gut microbial composition similarity compared to unrelated individuals. After residualizing against clinical covariates, 30 common operational taxonomic units were correlated between siblings across time points. These belonged to the bacterial classes Actinobacteria, Bacilli, Bacteroidia, Clostridia, Erysipelotrichia, and Negativicutes. Besides the influence of BMF and weight variables on the gut microbial diversity, our study identified gut microbial similarities between siblings that suggest genetic or shared maternal and environmental effects on the preterm infant gut microbiota. |
| first_indexed | 2024-03-09T03:20:48Z |
| format | Article |
| id | doaj.art-c758b0f0bf5e4b3ba0558c35a33b7036 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:20:48Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c758b0f0bf5e4b3ba0558c35a33b70362023-12-03T15:11:26ZengMDPI AGMicroorganisms2076-26072021-01-019227810.3390/microorganisms9020278The Effects of Genetic Relatedness on the Preterm Infant Gut MicrobiotaShen Jean Lim0Miriam Aguilar-Lopez1Christine Wetzel2Samia V. O. Dutra3Vanessa Bray4Maureen W. Groer5Sharon M. Donovan6Thao Ho7College of Nursing, University of South Florida, Tampa, FL 33612, USADivision of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, IL 61801, USANeonatology Division, Carle Hospital, Urbana, IL 61801, USACollege of Nursing, University of South Florida, Tampa, FL 33612, USACollege of Medicine, University of South Florida, Tampa, FL 33612, USACollege of Nursing, University of South Florida, Tampa, FL 33612, USADivision of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, IL 61801, USACollege of Medicine, University of South Florida, Tampa, FL 33612, USAThe preterm infant gut microbiota is influenced by environmental, endogenous, maternal, and genetic factors. Although siblings share similar gut microbial composition, it is not known how genetic relatedness affects alpha diversity and specific taxa abundances in preterm infants. We analyzed the 16S rRNA gene content of stool samples, ≤ and >3 weeks postnatal age, and clinical data from preterm multiplets and singletons at two Neonatal Intensive Care Units (NICUs), Tampa General Hospital (TGH; FL, USA) and Carle Hospital (IL, USA). Weeks on bovine milk-based fortifier (BMF) and weight gain velocity were significant predictors of alpha diversity. Alpha diversity between siblings were significantly correlated, particularly at ≤3 weeks postnatal age and in the TGH NICU, after controlling for clinical factors. Siblings shared higher gut microbial composition similarity compared to unrelated individuals. After residualizing against clinical covariates, 30 common operational taxonomic units were correlated between siblings across time points. These belonged to the bacterial classes Actinobacteria, Bacilli, Bacteroidia, Clostridia, Erysipelotrichia, and Negativicutes. Besides the influence of BMF and weight variables on the gut microbial diversity, our study identified gut microbial similarities between siblings that suggest genetic or shared maternal and environmental effects on the preterm infant gut microbiota.https://www.mdpi.com/2076-2607/9/2/278preterm infantgut microbiotahuman milktwinstriplets |
| spellingShingle | Shen Jean Lim Miriam Aguilar-Lopez Christine Wetzel Samia V. O. Dutra Vanessa Bray Maureen W. Groer Sharon M. Donovan Thao Ho The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota Microorganisms preterm infant gut microbiota human milk twins triplets |
| title | The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota |
| title_full | The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota |
| title_fullStr | The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota |
| title_full_unstemmed | The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota |
| title_short | The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota |
| title_sort | effects of genetic relatedness on the preterm infant gut microbiota |
| topic | preterm infant gut microbiota human milk twins triplets |
| url | https://www.mdpi.com/2076-2607/9/2/278 |
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