Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro

The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts...

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Main Authors: Svitlana Prylutska, Anna Grebinyk, Stanislav Ponomarenko, Defne Gövem, Vasyl Chumachenko, Nataliya Kutsevol, Mykola Petrovsky, Uwe Ritter, Marcus Frohme, Jacek Piosik, Yuriy Prylutskyy
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:International Journal of Molecular Sciences
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Online Access:https://www.mdpi.com/1422-0067/25/5/3069
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author Svitlana Prylutska
Anna Grebinyk
Stanislav Ponomarenko
Defne Gövem
Vasyl Chumachenko
Nataliya Kutsevol
Mykola Petrovsky
Uwe Ritter
Marcus Frohme
Jacek Piosik
Yuriy Prylutskyy
author_facet Svitlana Prylutska
Anna Grebinyk
Stanislav Ponomarenko
Defne Gövem
Vasyl Chumachenko
Nataliya Kutsevol
Mykola Petrovsky
Uwe Ritter
Marcus Frohme
Jacek Piosik
Yuriy Prylutskyy
author_sort Svitlana Prylutska
collection DOAJ
description The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology–Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated.
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spelling doaj.art-c759dd3b3cad4114b0ceebf638c60e7e2024-03-12T16:47:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255306910.3390/ijms25053069Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In VitroSvitlana Prylutska0Anna Grebinyk1Stanislav Ponomarenko2Defne Gövem3Vasyl Chumachenko4Nataliya Kutsevol5Mykola Petrovsky6Uwe Ritter7Marcus Frohme8Jacek Piosik9Yuriy Prylutskyy10Department of Plants Physiology, Biochemistry and Bioenergetics, National University of Life and Environmental Sciences of Ukraine, 03041 Kyiv, UkraineDeutsches Elektronen-Synchrotron DESY, Platanenallee 6, 15738 Zeuthen, GermanyDepartment of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, UkraineDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, GermanyDepartment of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, UkraineDepartment of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, UkraineDepartment of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, UkraineInstitute of Chemistry and Biotechnology, Technical University of Ilmenau, 98693 Ilmenau, GermanyDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, GermanyIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 80-307 Gdańsk, PolandDepartment of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, UkraineThe application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology–Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated.https://www.mdpi.com/1422-0067/25/5/3069star-like copolymerdrugLewis lung carcinomacytotoxicity
spellingShingle Svitlana Prylutska
Anna Grebinyk
Stanislav Ponomarenko
Defne Gövem
Vasyl Chumachenko
Nataliya Kutsevol
Mykola Petrovsky
Uwe Ritter
Marcus Frohme
Jacek Piosik
Yuriy Prylutskyy
Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
International Journal of Molecular Sciences
star-like copolymer
drug
Lewis lung carcinoma
cytotoxicity
title Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
title_full Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
title_fullStr Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
title_full_unstemmed Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
title_short Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
title_sort toxicity of water soluble d g pnipam polymers in a complex with chemotherapy drugs and mechanism of their action in vitro
topic star-like copolymer
drug
Lewis lung carcinoma
cytotoxicity
url https://www.mdpi.com/1422-0067/25/5/3069
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