Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor
Aptamer functionalized graphene field effect transistor (apta-GFET) is a versatile bio-sensing platform. However, the chemical inertness of graphene is still an obstacle for its large-scale applications and commercialization. In this work, reduced carboxyl-graphene oxide (rGO-COOH) is studied as a s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/10/6/388 |
| _version_ | 1818965091929817088 |
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| author | Fang Li Zhongrong Wang Yunfang Jia |
| author_facet | Fang Li Zhongrong Wang Yunfang Jia |
| author_sort | Fang Li |
| collection | DOAJ |
| description | Aptamer functionalized graphene field effect transistor (apta-GFET) is a versatile bio-sensing platform. However, the chemical inertness of graphene is still an obstacle for its large-scale applications and commercialization. In this work, reduced carboxyl-graphene oxide (rGO-COOH) is studied as a self-activated channel material in the screen-printed apta-GFETs for the first time. Examinations are carefully executed using lead-specific-aptamer as a proof-of-concept to demonstrate its functions in accommodating aptamer bio-probes and promoting the sensing reaction. The graphene-state, few-layer nano-structure, plenty of oxygen-containing groups and enhanced LSA immobilization of the rGO-COOH channel film are evidenced by X-ray photoelectron spectroscopy, Raman spectrum, UV-visible absorbance, atomic force microscopy and scanning electron microscope. Based on these characterizations, as well as a site-binding model based on solution-gated field effect transistor (SgFET) working principle, theoretical deductions for rGO-COOH enhanced apta-GFETs’ response are provided. Furthermore, detections for disturbing ions and real samples demonstrate the rGO-COOH channeled apta-GFET has a good specificity, a limit-of-detection of 0.001 ppb, and is in agreement with the conventional inductively coupled plasma mass spectrometry method. In conclusion, the careful examinations demonstrate rGO-COOH is a promising candidate as a self-activated channel material because of its merits of being independent of linking reagents, free from polymer residue and compatible with rapidly developed print-electronic technology. |
| first_indexed | 2024-12-20T13:11:30Z |
| format | Article |
| id | doaj.art-c75a5f7d4dcb4b5da1f174befb1c405f |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-20T13:11:30Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-c75a5f7d4dcb4b5da1f174befb1c405f2022-12-21T19:39:39ZengMDPI AGMicromachines2072-666X2019-06-0110638810.3390/mi10060388mi10060388Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer SensorFang Li0Zhongrong Wang1Yunfang Jia2College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300071, ChinaCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin 300071, ChinaCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin 300071, ChinaAptamer functionalized graphene field effect transistor (apta-GFET) is a versatile bio-sensing platform. However, the chemical inertness of graphene is still an obstacle for its large-scale applications and commercialization. In this work, reduced carboxyl-graphene oxide (rGO-COOH) is studied as a self-activated channel material in the screen-printed apta-GFETs for the first time. Examinations are carefully executed using lead-specific-aptamer as a proof-of-concept to demonstrate its functions in accommodating aptamer bio-probes and promoting the sensing reaction. The graphene-state, few-layer nano-structure, plenty of oxygen-containing groups and enhanced LSA immobilization of the rGO-COOH channel film are evidenced by X-ray photoelectron spectroscopy, Raman spectrum, UV-visible absorbance, atomic force microscopy and scanning electron microscope. Based on these characterizations, as well as a site-binding model based on solution-gated field effect transistor (SgFET) working principle, theoretical deductions for rGO-COOH enhanced apta-GFETs’ response are provided. Furthermore, detections for disturbing ions and real samples demonstrate the rGO-COOH channeled apta-GFET has a good specificity, a limit-of-detection of 0.001 ppb, and is in agreement with the conventional inductively coupled plasma mass spectrometry method. In conclusion, the careful examinations demonstrate rGO-COOH is a promising candidate as a self-activated channel material because of its merits of being independent of linking reagents, free from polymer residue and compatible with rapidly developed print-electronic technology.https://www.mdpi.com/2072-666X/10/6/388field effect transistorreduced carboxylate graphene oxideaptamersensorscreen print |
| spellingShingle | Fang Li Zhongrong Wang Yunfang Jia Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor Micromachines field effect transistor reduced carboxylate graphene oxide aptamer sensor screen print |
| title | Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor |
| title_full | Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor |
| title_fullStr | Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor |
| title_full_unstemmed | Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor |
| title_short | Reduced Carboxylate Graphene Oxide based Field Effect Transistor as Pb<sup>2+</sup> Aptamer Sensor |
| title_sort | reduced carboxylate graphene oxide based field effect transistor as pb sup 2 sup aptamer sensor |
| topic | field effect transistor reduced carboxylate graphene oxide aptamer sensor screen print |
| url | https://www.mdpi.com/2072-666X/10/6/388 |
| work_keys_str_mv | AT fangli reducedcarboxylategrapheneoxidebasedfieldeffecttransistoraspbsup2supaptamersensor AT zhongrongwang reducedcarboxylategrapheneoxidebasedfieldeffecttransistoraspbsup2supaptamersensor AT yunfangjia reducedcarboxylategrapheneoxidebasedfieldeffecttransistoraspbsup2supaptamersensor |