Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin
Mitochondria are key organelles of the cell because their main function is the capture of energy-rich substrates from the cytoplasm and oxidative cleavage with the generation of carbon dioxide and water, processes that are coupled with the synthesis of ATP. Mitochondria are subject to oxidative stre...
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MDPI AG
2020-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/10/437 |
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| author | Roman Krestinin Yulia Baburina Irina Odinokova Alexey Kruglov Irina Fadeeva Alena Zvyagina Linda Sotnikova Olga Krestinina |
| author_facet | Roman Krestinin Yulia Baburina Irina Odinokova Alexey Kruglov Irina Fadeeva Alena Zvyagina Linda Sotnikova Olga Krestinina |
| author_sort | Roman Krestinin |
| collection | DOAJ |
| description | Mitochondria are key organelles of the cell because their main function is the capture of energy-rich substrates from the cytoplasm and oxidative cleavage with the generation of carbon dioxide and water, processes that are coupled with the synthesis of ATP. Mitochondria are subject to oxidative stress through the formation of the mitochondrial permeability transition pore (mPTP). Various antioxidants are used to reduce damage caused by oxidative stress and to improve the protection of the antioxidant system. Astaxanthin (AST) is considered to be a dietary antioxidant, which is able to reduce oxidative stress and enhance the antioxidant defense system. In the present investigation, the effect of AST on the functional state of rat heart mitochondria impaired by isoproterenol (ISO) under mPTP functioning was examined. It was found that AST raised mitochondrial respiration, the Ca<sup>2+</sup> retention capacity (CRC), and the rate of TPP<sup>+</sup> influx in rat heart mitochondria (RHM) isolated from ISO-injected rats. However, the level of reactive oxygen species (ROS) production increased. In addition, the concentrations of cardiolipin (CL), Mn-SOD2, and the proteins regulating mPTP rose after the injection of ISO in RHM pretreated with AST. Based on the data obtained, we suggest that AST has a protective effect in rat heart mitochondria. |
| first_indexed | 2024-03-10T15:28:58Z |
| format | Article |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T15:28:58Z |
| publishDate | 2020-10-01 |
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| series | Biomedicines |
| spelling | doaj.art-c75aed42b44b44408e7aaf677aa163102023-11-20T17:49:10ZengMDPI AGBiomedicines2227-90592020-10-0181043710.3390/biomedicines8100437Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by AstaxanthinRoman Krestinin0Yulia Baburina1Irina Odinokova2Alexey Kruglov3Irina Fadeeva4Alena Zvyagina5Linda Sotnikova6Olga Krestinina7Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaMitochondria are key organelles of the cell because their main function is the capture of energy-rich substrates from the cytoplasm and oxidative cleavage with the generation of carbon dioxide and water, processes that are coupled with the synthesis of ATP. Mitochondria are subject to oxidative stress through the formation of the mitochondrial permeability transition pore (mPTP). Various antioxidants are used to reduce damage caused by oxidative stress and to improve the protection of the antioxidant system. Astaxanthin (AST) is considered to be a dietary antioxidant, which is able to reduce oxidative stress and enhance the antioxidant defense system. In the present investigation, the effect of AST on the functional state of rat heart mitochondria impaired by isoproterenol (ISO) under mPTP functioning was examined. It was found that AST raised mitochondrial respiration, the Ca<sup>2+</sup> retention capacity (CRC), and the rate of TPP<sup>+</sup> influx in rat heart mitochondria (RHM) isolated from ISO-injected rats. However, the level of reactive oxygen species (ROS) production increased. In addition, the concentrations of cardiolipin (CL), Mn-SOD2, and the proteins regulating mPTP rose after the injection of ISO in RHM pretreated with AST. Based on the data obtained, we suggest that AST has a protective effect in rat heart mitochondria.https://www.mdpi.com/2227-9059/8/10/437rat heart mitochondria (RHM)mitochondrial permeability transition pore (mPTP)regulatory proteinsreactive oxygen species (ROS)cardiolipin (CL) |
| spellingShingle | Roman Krestinin Yulia Baburina Irina Odinokova Alexey Kruglov Irina Fadeeva Alena Zvyagina Linda Sotnikova Olga Krestinina Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin Biomedicines rat heart mitochondria (RHM) mitochondrial permeability transition pore (mPTP) regulatory proteins reactive oxygen species (ROS) cardiolipin (CL) |
| title | Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin |
| title_full | Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin |
| title_fullStr | Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin |
| title_full_unstemmed | Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin |
| title_short | Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin |
| title_sort | isoproterenol induced permeability transition pore related dysfunction of heart mitochondria is attenuated by astaxanthin |
| topic | rat heart mitochondria (RHM) mitochondrial permeability transition pore (mPTP) regulatory proteins reactive oxygen species (ROS) cardiolipin (CL) |
| url | https://www.mdpi.com/2227-9059/8/10/437 |
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