Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province
The stratified acidic pit lake formed by the confluence of acid mine drainage has a unique ecological niche and is a model system for extreme microbial studies. Eukaryotes are a component of the AMD community, with the main members including microalgae, fungi, and a small number of protozoa. In this...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/979 |
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| author | Xin Pan Zhengbo Yue Zhixiang She Xiao He Shaoping Wang Xin Chuai Jin Wang |
| author_facet | Xin Pan Zhengbo Yue Zhixiang She Xiao He Shaoping Wang Xin Chuai Jin Wang |
| author_sort | Xin Pan |
| collection | DOAJ |
| description | The stratified acidic pit lake formed by the confluence of acid mine drainage has a unique ecological niche and is a model system for extreme microbial studies. Eukaryotes are a component of the AMD community, with the main members including microalgae, fungi, and a small number of protozoa. In this study, we analyzed the structural traits and interactions of eukaryotes (primarily fungi and microalgae) in acidic pit lakes subjected to environmental gradients. Based on the findings, microalgae and fungi were found to dominate different water layers. Specifically, Chlorophyta showed dominance in the well-lit aerobic surface layer, whereas Basidiomycota was more abundant in the dark anoxic lower layer. Co-occurrence network analysis showed that reciprocal relationships between fungi and microalgae were prevalent in extremely acidic environments. Highly connected taxa within this network were <i>Chlamydomonadaceae</i>, <i>Sporidiobolaceae</i>, <i>Filobasidiaceae,</i> and unclassified Eukaryotes. Redundancy analysis (RDA) and random forest models revealed that Chlorophyta and Basidiomycota responded strongly to environmental gradients. Further analysis indicated that eukaryotic community structure was mainly determined by nutrient and metal concentrations. This study investigates the potential symbiosis between fungi and microalgae in the acidic pit lake, providing valuable insights for future eukaryotic biodiversity studies on AMD remediation. |
| first_indexed | 2024-03-11T04:43:54Z |
| format | Article |
| id | doaj.art-c75c29dd341246a2b71a7335fba34736 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:54Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-c75c29dd341246a2b71a7335fba347362023-11-17T20:33:17ZengMDPI AGMicroorganisms2076-26072023-04-0111497910.3390/microorganisms11040979Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui ProvinceXin Pan0Zhengbo Yue1Zhixiang She2Xiao He3Shaoping Wang4Xin Chuai5Jin Wang6School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, ChinaNanshan Mining Company Ltd., Anhui Maanshan Iron and Steel Mining Resources Group, Maanshan 243000, ChinaNanshan Mining Company Ltd., Anhui Maanshan Iron and Steel Mining Resources Group, Maanshan 243000, ChinaSchool of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, ChinaThe stratified acidic pit lake formed by the confluence of acid mine drainage has a unique ecological niche and is a model system for extreme microbial studies. Eukaryotes are a component of the AMD community, with the main members including microalgae, fungi, and a small number of protozoa. In this study, we analyzed the structural traits and interactions of eukaryotes (primarily fungi and microalgae) in acidic pit lakes subjected to environmental gradients. Based on the findings, microalgae and fungi were found to dominate different water layers. Specifically, Chlorophyta showed dominance in the well-lit aerobic surface layer, whereas Basidiomycota was more abundant in the dark anoxic lower layer. Co-occurrence network analysis showed that reciprocal relationships between fungi and microalgae were prevalent in extremely acidic environments. Highly connected taxa within this network were <i>Chlamydomonadaceae</i>, <i>Sporidiobolaceae</i>, <i>Filobasidiaceae,</i> and unclassified Eukaryotes. Redundancy analysis (RDA) and random forest models revealed that Chlorophyta and Basidiomycota responded strongly to environmental gradients. Further analysis indicated that eukaryotic community structure was mainly determined by nutrient and metal concentrations. This study investigates the potential symbiosis between fungi and microalgae in the acidic pit lake, providing valuable insights for future eukaryotic biodiversity studies on AMD remediation.https://www.mdpi.com/2076-2607/11/4/979acid mine drainagepit lakeeukaryotes structuresco-occurrence networkfungi-microalgae interaction |
| spellingShingle | Xin Pan Zhengbo Yue Zhixiang She Xiao He Shaoping Wang Xin Chuai Jin Wang Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province Microorganisms acid mine drainage pit lake eukaryotes structures co-occurrence network fungi-microalgae interaction |
| title | Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province |
| title_full | Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province |
| title_fullStr | Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province |
| title_full_unstemmed | Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province |
| title_short | Eukaryotic Community Structure and Interspecific Interactions in a Stratified Acidic Pit Lake Water in Anhui Province |
| title_sort | eukaryotic community structure and interspecific interactions in a stratified acidic pit lake water in anhui province |
| topic | acid mine drainage pit lake eukaryotes structures co-occurrence network fungi-microalgae interaction |
| url | https://www.mdpi.com/2076-2607/11/4/979 |
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