Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China
According to recent investigations, the proportion of mastitis caused by environmental pathogens, such as <i>K. pneumoniae</i>, has increased. In this research, the epidemiology of pathogens in milk samples collected from four farms in the Jiangsu Province was carried out. The results sh...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2077-0472/13/10/1984 |
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| author | Wendi Cao Yi Xu Yicai Huang Tianle Xu |
| author_facet | Wendi Cao Yi Xu Yicai Huang Tianle Xu |
| author_sort | Wendi Cao |
| collection | DOAJ |
| description | According to recent investigations, the proportion of mastitis caused by environmental pathogens, such as <i>K. pneumoniae</i>, has increased. In this research, the epidemiology of pathogens in milk samples collected from four farms in the Jiangsu Province was carried out. The results show that 16 pathogens were detected in 186 positive milk samples. It was found that <i>K. pneumoniae</i> had the lowest sensitivity to penicillin (0%) and amoxicillin (4%) compared to its sensitivity to gentamicin (92%) and piperacillin (89%). A total of eight ESBL-producing strains were detected. Crystal violet staining showed that 46 of the 68 isolates of <i>K. pneumoniae</i> had strong biofilm-forming ability, which was related to the tetracycline resistance phenotype (<i>p</i> < 0.05). The detection rate of the ESBL-resistant gene (<i>bla<sub>SHV</sub></i>) reached 100%. The results show that resistance genes <i>bla<sub>VIM</sub></i>, <i>bla<sub>OXA-10</sub></i>, and <i>bla<sub>TEM</sub></i> were correlated with drug-resistance phenotypes to varying degrees. The present study indicates the prevalence of bovine mastitis-derived pathogens in part of Jiangsu Province and reveals the distribution of β-lactam resistance genes and the strong biofilm-forming ability of <i>K. pneumoniae</i> and its relationship with tetracycline resistance. This study provided theoretical support and guidance for rational drug use and disease prevention and control on farms. |
| first_indexed | 2024-03-10T21:31:42Z |
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| language | English |
| last_indexed | 2024-03-10T21:31:42Z |
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| spelling | doaj.art-c75e655e38ff4f2d842bb42033925a592023-11-19T15:19:29ZengMDPI AGAgriculture2077-04722023-10-011310198410.3390/agriculture13101984Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, ChinaWendi Cao0Yi Xu1Yicai Huang2Tianle Xu3Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaAccording to recent investigations, the proportion of mastitis caused by environmental pathogens, such as <i>K. pneumoniae</i>, has increased. In this research, the epidemiology of pathogens in milk samples collected from four farms in the Jiangsu Province was carried out. The results show that 16 pathogens were detected in 186 positive milk samples. It was found that <i>K. pneumoniae</i> had the lowest sensitivity to penicillin (0%) and amoxicillin (4%) compared to its sensitivity to gentamicin (92%) and piperacillin (89%). A total of eight ESBL-producing strains were detected. Crystal violet staining showed that 46 of the 68 isolates of <i>K. pneumoniae</i> had strong biofilm-forming ability, which was related to the tetracycline resistance phenotype (<i>p</i> < 0.05). The detection rate of the ESBL-resistant gene (<i>bla<sub>SHV</sub></i>) reached 100%. The results show that resistance genes <i>bla<sub>VIM</sub></i>, <i>bla<sub>OXA-10</sub></i>, and <i>bla<sub>TEM</sub></i> were correlated with drug-resistance phenotypes to varying degrees. The present study indicates the prevalence of bovine mastitis-derived pathogens in part of Jiangsu Province and reveals the distribution of β-lactam resistance genes and the strong biofilm-forming ability of <i>K. pneumoniae</i> and its relationship with tetracycline resistance. This study provided theoretical support and guidance for rational drug use and disease prevention and control on farms.https://www.mdpi.com/2077-0472/13/10/1984bovine mastitis<i>Klebsiella pneumoniae</i>biofilmdrug-resistance gene |
| spellingShingle | Wendi Cao Yi Xu Yicai Huang Tianle Xu Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China Agriculture bovine mastitis <i>Klebsiella pneumoniae</i> biofilm drug-resistance gene |
| title | Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China |
| title_full | Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China |
| title_fullStr | Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China |
| title_full_unstemmed | Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China |
| title_short | Isolation of Pathogenic Bacteria from Dairy Cow Mastitis and Correlation of Biofilm Formation and Drug Resistance of <i>Klebsiella pneumoniae</i> in Jiangsu, China |
| title_sort | isolation of pathogenic bacteria from dairy cow mastitis and correlation of biofilm formation and drug resistance of i klebsiella pneumoniae i in jiangsu china |
| topic | bovine mastitis <i>Klebsiella pneumoniae</i> biofilm drug-resistance gene |
| url | https://www.mdpi.com/2077-0472/13/10/1984 |
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