Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds
Background: Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and...
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MDPI AG
2023-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/3/880 |
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| author | Aswathy Ravindran Girija Xanthe Strudwick Sivakumar Balasubramanian Vivekanandan Palaninathan Sakthikumar Dasappan Nair Allison J. Cowin |
| author_facet | Aswathy Ravindran Girija Xanthe Strudwick Sivakumar Balasubramanian Vivekanandan Palaninathan Sakthikumar Dasappan Nair Allison J. Cowin |
| author_sort | Aswathy Ravindran Girija |
| collection | DOAJ |
| description | Background: Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and porous scaffolds once biofunctionalized with collagen. Methods: The in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in full-thickness mouse wounds was assessed by cellular infiltration, wound closure and re-epithelialization and ECM deposition. Results: Early indications suggested unmodified, smooth PLLA scaffolds perform poorly, with limited cellular infiltration and matrix deposition around the scaffold, the largest wound area, a significantly larger panniculus gape, and lowest re-epithelialization; however, by day 14, no significant differences were observed. Collagen biofunctionalization may improve healing, as collagen-functionalized smooth scaffolds were smallest overall, and collagen-functionalized porous scaffolds were smaller than non-functionalized porous scaffolds; the highest re-epithelialization was observed in wounds treated with collagen-functionalized scaffolds. Conclusion: Our results suggest that limited incorporation of smooth PLLA scaffolds into the healing wound occurs, and that altering surface topology, particularly by utilizing collagen biofunctionalization, may improve healing. The differing performance of the unmodified scaffolds in the in vitro versus in vivo studies demonstrates the importance of preclinical testing. |
| first_indexed | 2024-03-11T06:02:37Z |
| format | Article |
| id | doaj.art-c75f7567921d488fab118cb3839132be |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:02:37Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-c75f7567921d488fab118cb3839132be2023-11-17T13:15:42ZengMDPI AGPharmaceutics1999-49232023-03-0115388010.3390/pharmaceutics15030880Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness WoundsAswathy Ravindran Girija0Xanthe Strudwick1Sivakumar Balasubramanian2Vivekanandan Palaninathan3Sakthikumar Dasappan Nair4Allison J. Cowin5Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaBio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe 350-0815, Saitama, JapanBio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe 350-0815, Saitama, JapanFuture Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaBackground: Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and porous scaffolds once biofunctionalized with collagen. Methods: The in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in full-thickness mouse wounds was assessed by cellular infiltration, wound closure and re-epithelialization and ECM deposition. Results: Early indications suggested unmodified, smooth PLLA scaffolds perform poorly, with limited cellular infiltration and matrix deposition around the scaffold, the largest wound area, a significantly larger panniculus gape, and lowest re-epithelialization; however, by day 14, no significant differences were observed. Collagen biofunctionalization may improve healing, as collagen-functionalized smooth scaffolds were smallest overall, and collagen-functionalized porous scaffolds were smaller than non-functionalized porous scaffolds; the highest re-epithelialization was observed in wounds treated with collagen-functionalized scaffolds. Conclusion: Our results suggest that limited incorporation of smooth PLLA scaffolds into the healing wound occurs, and that altering surface topology, particularly by utilizing collagen biofunctionalization, may improve healing. The differing performance of the unmodified scaffolds in the in vitro versus in vivo studies demonstrates the importance of preclinical testing.https://www.mdpi.com/1999-4923/15/3/880poly(L-lactic acid)electrospun scaffoldswound healingcollagenbiofunctionalization |
| spellingShingle | Aswathy Ravindran Girija Xanthe Strudwick Sivakumar Balasubramanian Vivekanandan Palaninathan Sakthikumar Dasappan Nair Allison J. Cowin Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds Pharmaceutics poly(L-lactic acid) electrospun scaffolds wound healing collagen biofunctionalization |
| title | Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds |
| title_full | Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds |
| title_fullStr | Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds |
| title_full_unstemmed | Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds |
| title_short | Collagen Functionalization of Polymeric Electrospun Scaffolds to Improve Integration into Full-Thickness Wounds |
| title_sort | collagen functionalization of polymeric electrospun scaffolds to improve integration into full thickness wounds |
| topic | poly(L-lactic acid) electrospun scaffolds wound healing collagen biofunctionalization |
| url | https://www.mdpi.com/1999-4923/15/3/880 |
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