Cultivable Microbiome Approach Applied to Cervical Cancer Exploration
Traditional microbiological methodology is valuable and essential for microbiota composition description and microbe role assignations at different anatomical sites, including cervical and vaginal tissues; that, combined with molecular biology strategies and modern identification approaches, could g...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/16/2/314 |
| _version_ | 1797344511218679808 |
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| author | Irma Berenice Mulato-Briones Ismael Olan Rodriguez-Ildefonso Julián Antonio Jiménez-Tenorio Patricia Isidra Cauich-Sánchez María del Socorro Méndez-Tovar Gerardo Aparicio-Ozores María Yicel Bautista-Hernández Juan Francisco González-Parra Jesús Cruz-Hernández Ricardo López-Romero Teresita María del Rosario Rojas-Sánchez Raúl García-Palacios Ónix Garay-Villar Teresa Apresa-García Juan López-Esparza Daniel Marrero Juan Arturo Castelán-Vega Alicia Jiménez-Alberto Mauricio Salcedo Rosa María Ribas-Aparicio |
| author_facet | Irma Berenice Mulato-Briones Ismael Olan Rodriguez-Ildefonso Julián Antonio Jiménez-Tenorio Patricia Isidra Cauich-Sánchez María del Socorro Méndez-Tovar Gerardo Aparicio-Ozores María Yicel Bautista-Hernández Juan Francisco González-Parra Jesús Cruz-Hernández Ricardo López-Romero Teresita María del Rosario Rojas-Sánchez Raúl García-Palacios Ónix Garay-Villar Teresa Apresa-García Juan López-Esparza Daniel Marrero Juan Arturo Castelán-Vega Alicia Jiménez-Alberto Mauricio Salcedo Rosa María Ribas-Aparicio |
| author_sort | Irma Berenice Mulato-Briones |
| collection | DOAJ |
| description | Traditional microbiological methodology is valuable and essential for microbiota composition description and microbe role assignations at different anatomical sites, including cervical and vaginal tissues; that, combined with molecular biology strategies and modern identification approaches, could give a better perspective of the microbiome under different circumstances. This pilot work aimed to describe the differences in microbiota composition in non-cancer women and women with cervical cancer through a culturomics approach combining culture techniques with Vitek mass spectrometry and 16S rDNA sequencing. To determine the possible differences, diverse statistical, diversity, and multivariate analyses were applied; the results indicated a different microbiota composition between non-cancer women and cervical cancer patients. The Firmicutes phylum dominated the non-cancer (NC) group, whereas the cervical cancer (CC) group was characterized by the predominance of Firmicutes and Proteobacteria phyla; there was a depletion of lactic acid bacteria, an increase in the diversity of anaerobes, and opportunistic and non-typical human microbiota isolates were present. In this context, we hypothesize and propose a model in which microbial composition and dynamics may be essential for maintaining the balance in the cervical microenvironment or can be pro-oncogenesis microenvironmental mediators in a process called <i>Ying-Yang</i> or have a protagonist/antagonist microbiota role. |
| first_indexed | 2024-03-08T11:03:40Z |
| format | Article |
| id | doaj.art-c75f87407f79403ab1418687835d2e85 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T11:03:40Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c75f87407f79403ab1418687835d2e852024-01-26T15:35:37ZengMDPI AGCancers2072-66942024-01-0116231410.3390/cancers16020314Cultivable Microbiome Approach Applied to Cervical Cancer ExplorationIrma Berenice Mulato-Briones0Ismael Olan Rodriguez-Ildefonso1Julián Antonio Jiménez-Tenorio2Patricia Isidra Cauich-Sánchez3María del Socorro Méndez-Tovar4Gerardo Aparicio-Ozores5María Yicel Bautista-Hernández6Juan Francisco González-Parra7Jesús Cruz-Hernández8Ricardo López-Romero9Teresita María del Rosario Rojas-Sánchez10Raúl García-Palacios11Ónix Garay-Villar12Teresa Apresa-García13Juan López-Esparza14Daniel Marrero15Juan Arturo Castelán-Vega16Alicia Jiménez-Alberto17Mauricio Salcedo18Rosa María Ribas-Aparicio19Laboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratorio de Bacteriología Clínica, Hospital General, Centro Médico Nacional “La Raza”, IMSS, Mexico City 02990, MexicoLaboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoUnidad de Radiología, Hospital General de México “Dr. Eduardo Liceaga”, Secretaría de Salud, Mexico City 07300, MexicoUnidad de Radiología, Hospital General de México “Dr. Eduardo Liceaga”, Secretaría de Salud, Mexico City 07300, MexicoUnidad de Radiología, Hospital General de México “Dr. Eduardo Liceaga”, Secretaría de Salud, Mexico City 07300, MexicoUnidad de Investigación en Biomedicina y Oncología Genómica (UIBOG), del Hospital de Gineco Pediatría No. 3A, del Instituto Mexicano del Seguro Social (IMSS), Mexico City 07300, MexicoClínica de la Mujer, Hospital Privado, Mexico City 06760, MexicoClínica de Atención a la Mujer, Mexico City 03310, MexicoDepartamento de Braquiterapia, Hospital de Oncología, Centro Médico Nacional Siglo XXI, IMSS (DBHOCMN-IMSS), Mexico City 07300, MexicoUnidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, IMSS, Mexico City 07300, MexicoLaboratorio de H109, Academia de Microbiología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez 32310, MexicoUnidad de Investigación Médica en Enfermedades Endocrinas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Mexico City 07300, MexicoLaboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoUnidad de Investigación en Biomedicina y Oncología Genómica (UIBOG), del Hospital de Gineco Pediatría No. 3A, del Instituto Mexicano del Seguro Social (IMSS), Mexico City 07300, MexicoLaboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoTraditional microbiological methodology is valuable and essential for microbiota composition description and microbe role assignations at different anatomical sites, including cervical and vaginal tissues; that, combined with molecular biology strategies and modern identification approaches, could give a better perspective of the microbiome under different circumstances. This pilot work aimed to describe the differences in microbiota composition in non-cancer women and women with cervical cancer through a culturomics approach combining culture techniques with Vitek mass spectrometry and 16S rDNA sequencing. To determine the possible differences, diverse statistical, diversity, and multivariate analyses were applied; the results indicated a different microbiota composition between non-cancer women and cervical cancer patients. The Firmicutes phylum dominated the non-cancer (NC) group, whereas the cervical cancer (CC) group was characterized by the predominance of Firmicutes and Proteobacteria phyla; there was a depletion of lactic acid bacteria, an increase in the diversity of anaerobes, and opportunistic and non-typical human microbiota isolates were present. In this context, we hypothesize and propose a model in which microbial composition and dynamics may be essential for maintaining the balance in the cervical microenvironment or can be pro-oncogenesis microenvironmental mediators in a process called <i>Ying-Yang</i> or have a protagonist/antagonist microbiota role.https://www.mdpi.com/2072-6694/16/2/314microbiologyaerobic and anaerobic culturescervical epitheliumcervical cancerculturomics |
| spellingShingle | Irma Berenice Mulato-Briones Ismael Olan Rodriguez-Ildefonso Julián Antonio Jiménez-Tenorio Patricia Isidra Cauich-Sánchez María del Socorro Méndez-Tovar Gerardo Aparicio-Ozores María Yicel Bautista-Hernández Juan Francisco González-Parra Jesús Cruz-Hernández Ricardo López-Romero Teresita María del Rosario Rojas-Sánchez Raúl García-Palacios Ónix Garay-Villar Teresa Apresa-García Juan López-Esparza Daniel Marrero Juan Arturo Castelán-Vega Alicia Jiménez-Alberto Mauricio Salcedo Rosa María Ribas-Aparicio Cultivable Microbiome Approach Applied to Cervical Cancer Exploration Cancers microbiology aerobic and anaerobic cultures cervical epithelium cervical cancer culturomics |
| title | Cultivable Microbiome Approach Applied to Cervical Cancer Exploration |
| title_full | Cultivable Microbiome Approach Applied to Cervical Cancer Exploration |
| title_fullStr | Cultivable Microbiome Approach Applied to Cervical Cancer Exploration |
| title_full_unstemmed | Cultivable Microbiome Approach Applied to Cervical Cancer Exploration |
| title_short | Cultivable Microbiome Approach Applied to Cervical Cancer Exploration |
| title_sort | cultivable microbiome approach applied to cervical cancer exploration |
| topic | microbiology aerobic and anaerobic cultures cervical epithelium cervical cancer culturomics |
| url | https://www.mdpi.com/2072-6694/16/2/314 |
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