Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach
Computed tomography (CT) is the standard method to evaluate Lipiodol deposition after transarterial embolization (TAE) for a long period. However, iodine but not Lipiodol can be observed on CT. A minimally invasive other method to detect Lipiodol has been needed to evaluate accurate evaluation after...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/10/9/901 |
| _version_ | 1797520208892526592 |
|---|---|
| author | Natsuhiko Saito Toshihiro Tanaka Kiyoyuki Minamiguchi Ryosuke Taiji Hideyuki Nishiofuku Takeshi Matsumoto Toshiko Hirai Kimihiko Kichikawa Naoki Kawahara Daiki Matsuda Iwaki Akiyama |
| author_facet | Natsuhiko Saito Toshihiro Tanaka Kiyoyuki Minamiguchi Ryosuke Taiji Hideyuki Nishiofuku Takeshi Matsumoto Toshiko Hirai Kimihiko Kichikawa Naoki Kawahara Daiki Matsuda Iwaki Akiyama |
| author_sort | Natsuhiko Saito |
| collection | DOAJ |
| description | Computed tomography (CT) is the standard method to evaluate Lipiodol deposition after transarterial embolization (TAE) for a long period. However, iodine but not Lipiodol can be observed on CT. A minimally invasive other method to detect Lipiodol has been needed to evaluate accurate evaluation after procedure. The purpose of this study was to evaluate the efficacy of using the rate of change in sound velocity caused by ultrasonic heating to reflect Lipiodol accumulation after TAE in a rat liver tumor model. We analyzed the association of this developed technique with CT images and histological findings. Eight rats bearing N1S1 cells were prepared. After confirmation of tumor development in a rat liver, Lipiodol was injected via the hepatic artery. Seven days after TAE, CT scan and sound velocity changes caused by ultrasonic heating were measured, and then the rats were sacrificed. An ultrasonic pulse-echo method was used to measure the sound velocity. The temperature coefficient of the sound velocity in each treated tumor was evaluated and compared with the mean CT value and the histological Lipiodol accumulation ratio. Pearson’s correlation coefficients were calculated to assess the correlation between the measured values. The correlation coefficient (r) of the mean CT value and histological Lipiodol accumulation ratio was 0.835 (<i>p</i> = 0.010), which was considered statistically significant. Also, those of the temperature coefficient of the sound velocity and the histological Lipiodol accumulation ratio were statistically significant (r = 0.804; <i>p</i> = 0.016). To our knowledge, this is the first study that reported the efficacy of ultrasonic heating to detect Lipiodol accumulation in rat liver tumors after TAE. Our results suggest that the rate of change in sound velocity caused by ultrasonic heating can be used to evaluate Lipiodol accumulation in liver tumors after TAE, and thus could represent an alternative to CT in this application. This new innovative technique is easy to treat and less invasive in terms of avoiding radiation compared with CT. |
| first_indexed | 2024-03-10T07:53:35Z |
| format | Article |
| id | doaj.art-c760f31167504288a13c260f05dadf8d |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T07:53:35Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-c760f31167504288a13c260f05dadf8d2023-11-22T12:05:57ZengMDPI AGBiology2079-77372021-09-0110990110.3390/biology10090901Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo ApproachNatsuhiko Saito0Toshihiro Tanaka1Kiyoyuki Minamiguchi2Ryosuke Taiji3Hideyuki Nishiofuku4Takeshi Matsumoto5Toshiko Hirai6Kimihiko Kichikawa7Naoki Kawahara8Daiki Matsuda9Iwaki Akiyama10Department of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Radiology and Nuclear Medicine, Nara Medical University, Kashihara Nara 634-8521, JapanDepartment of Medical Ultrasound Research Center, Doshisha University, Kyotanabe Kyoto 610-0321, JapanDepartment of Medical Ultrasound Research Center, Doshisha University, Kyotanabe Kyoto 610-0321, JapanDepartment of Medical Ultrasound Research Center, Doshisha University, Kyotanabe Kyoto 610-0321, JapanComputed tomography (CT) is the standard method to evaluate Lipiodol deposition after transarterial embolization (TAE) for a long period. However, iodine but not Lipiodol can be observed on CT. A minimally invasive other method to detect Lipiodol has been needed to evaluate accurate evaluation after procedure. The purpose of this study was to evaluate the efficacy of using the rate of change in sound velocity caused by ultrasonic heating to reflect Lipiodol accumulation after TAE in a rat liver tumor model. We analyzed the association of this developed technique with CT images and histological findings. Eight rats bearing N1S1 cells were prepared. After confirmation of tumor development in a rat liver, Lipiodol was injected via the hepatic artery. Seven days after TAE, CT scan and sound velocity changes caused by ultrasonic heating were measured, and then the rats were sacrificed. An ultrasonic pulse-echo method was used to measure the sound velocity. The temperature coefficient of the sound velocity in each treated tumor was evaluated and compared with the mean CT value and the histological Lipiodol accumulation ratio. Pearson’s correlation coefficients were calculated to assess the correlation between the measured values. The correlation coefficient (r) of the mean CT value and histological Lipiodol accumulation ratio was 0.835 (<i>p</i> = 0.010), which was considered statistically significant. Also, those of the temperature coefficient of the sound velocity and the histological Lipiodol accumulation ratio were statistically significant (r = 0.804; <i>p</i> = 0.016). To our knowledge, this is the first study that reported the efficacy of ultrasonic heating to detect Lipiodol accumulation in rat liver tumors after TAE. Our results suggest that the rate of change in sound velocity caused by ultrasonic heating can be used to evaluate Lipiodol accumulation in liver tumors after TAE, and thus could represent an alternative to CT in this application. This new innovative technique is easy to treat and less invasive in terms of avoiding radiation compared with CT.https://www.mdpi.com/2079-7737/10/9/901ultrasonic heatingtemperature coefficient of the sound velocityTACETAELipiodolliver tumor |
| spellingShingle | Natsuhiko Saito Toshihiro Tanaka Kiyoyuki Minamiguchi Ryosuke Taiji Hideyuki Nishiofuku Takeshi Matsumoto Toshiko Hirai Kimihiko Kichikawa Naoki Kawahara Daiki Matsuda Iwaki Akiyama Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach Biology ultrasonic heating temperature coefficient of the sound velocity TACE TAE Lipiodol liver tumor |
| title | Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach |
| title_full | Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach |
| title_fullStr | Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach |
| title_full_unstemmed | Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach |
| title_short | Ultrasonic Heating Detects Lipiodol Deposition within Liver Tumors after Transarterial Embolization: An In Vivo Approach |
| title_sort | ultrasonic heating detects lipiodol deposition within liver tumors after transarterial embolization an in vivo approach |
| topic | ultrasonic heating temperature coefficient of the sound velocity TACE TAE Lipiodol liver tumor |
| url | https://www.mdpi.com/2079-7737/10/9/901 |
| work_keys_str_mv | AT natsuhikosaito ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT toshihirotanaka ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT kiyoyukiminamiguchi ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT ryosuketaiji ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT hideyukinishiofuku ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT takeshimatsumoto ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT toshikohirai ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT kimihikokichikawa ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT naokikawahara ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT daikimatsuda ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach AT iwakiakiyama ultrasonicheatingdetectslipiodoldepositionwithinlivertumorsaftertransarterialembolizationaninvivoapproach |