Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study
Abstract: Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case–control study was to investigate the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Pesquisa Odontológica
2019-04-01
|
| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100224&lng=en&tlng=en |
| _version_ | 1819182489486229504 |
|---|---|
| author | Petra Borilova Linhartova Julius Janos Hana Poskerova Denisa Kavrikova Antonin Fassmann Ladislav Dusek Lydie Izakovicova Holla |
| author_facet | Petra Borilova Linhartova Julius Janos Hana Poskerova Denisa Kavrikova Antonin Fassmann Ladislav Dusek Lydie Izakovicova Holla |
| author_sort | Petra Borilova Linhartova |
| collection | DOAJ |
| description | Abstract: Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case–control study was to investigate the possible impacts of adiponectin (ADIPOQ), leptin (LEP) and its receptor (LEPR), and resistin (RETN) on the etiopathogenesis of CP. Examinations were performed on 118 non–periodontitis healthy subjects (healthy controls, HC), 205 healthy individuals with CP (H + CP) and 86 type 2 diabetes patients with CP (T2DM + CP). Variants within the ADIPOQ (rs2241766, rs1501299), LEP (rs13228377, rs2167270), LEP receptor (rs1805096), and RETN (rs1862513) genes were determined by qPCR. In addition, the plasma levels of ADIPOQ, LEP, and RETN were analysed by ELISA for 80 individuals. The genotype frequencies of the SNP ADIPOQ +45G/T (rs2241766) differed between the HC and H + CP groups (p=0.03, pcorr>0.05), and carriers of the TT genotype had a lower risk of developing CP compared to carriers of the GG or TG genotypes (p<0.01, pcorr>0.05). However, there were no significant differences in the plasma levels of ADIPOQ, LEP or RETN between the study groups (p > 0.05). Plasma levels of the adipokines were also independent of the gene profiles (p > 0.05). Adipokine plasma levels did not change in patients with H + CP/T2DM + CP compared to HC, but we did identify a specific polymorphism in the ADIPOQ gene that was associated with CP. Although the ADIPOQ +45G/T (rs2241766) gene variant may be a candidate biomarker for CP, further research is required in larger populations with different ethnic backgrounds before any final conclusions can be drawn about the role of this gene in CP. |
| first_indexed | 2024-12-22T22:46:57Z |
| format | Article |
| id | doaj.art-c7652aa228d24e7b977d7945958e58f3 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-22T22:46:57Z |
| publishDate | 2019-04-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-c7652aa228d24e7b977d7945958e58f32022-12-21T18:10:03ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072019-04-0133010.1590/1807-3107bor-2019.vol33.0034S1806-83242019000100224Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control studyPetra Borilova LinhartovaJulius JanosHana PoskerovaDenisa KavrikovaAntonin FassmannLadislav DusekLydie Izakovicova HollaAbstract: Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case–control study was to investigate the possible impacts of adiponectin (ADIPOQ), leptin (LEP) and its receptor (LEPR), and resistin (RETN) on the etiopathogenesis of CP. Examinations were performed on 118 non–periodontitis healthy subjects (healthy controls, HC), 205 healthy individuals with CP (H + CP) and 86 type 2 diabetes patients with CP (T2DM + CP). Variants within the ADIPOQ (rs2241766, rs1501299), LEP (rs13228377, rs2167270), LEP receptor (rs1805096), and RETN (rs1862513) genes were determined by qPCR. In addition, the plasma levels of ADIPOQ, LEP, and RETN were analysed by ELISA for 80 individuals. The genotype frequencies of the SNP ADIPOQ +45G/T (rs2241766) differed between the HC and H + CP groups (p=0.03, pcorr>0.05), and carriers of the TT genotype had a lower risk of developing CP compared to carriers of the GG or TG genotypes (p<0.01, pcorr>0.05). However, there were no significant differences in the plasma levels of ADIPOQ, LEP or RETN between the study groups (p > 0.05). Plasma levels of the adipokines were also independent of the gene profiles (p > 0.05). Adipokine plasma levels did not change in patients with H + CP/T2DM + CP compared to HC, but we did identify a specific polymorphism in the ADIPOQ gene that was associated with CP. Although the ADIPOQ +45G/T (rs2241766) gene variant may be a candidate biomarker for CP, further research is required in larger populations with different ethnic backgrounds before any final conclusions can be drawn about the role of this gene in CP.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100224&lng=en&tlng=enChronic PeriodontitisDiabetes Mellitus, Type 2Adipokines |
| spellingShingle | Petra Borilova Linhartova Julius Janos Hana Poskerova Denisa Kavrikova Antonin Fassmann Ladislav Dusek Lydie Izakovicova Holla Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study Brazilian Oral Research Chronic Periodontitis Diabetes Mellitus, Type 2 Adipokines |
| title | Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study |
| title_full | Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study |
| title_fullStr | Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study |
| title_full_unstemmed | Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study |
| title_short | Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study |
| title_sort | adipokine gene variability and plasma levels in patients with chronic periodontitis a case control study |
| topic | Chronic Periodontitis Diabetes Mellitus, Type 2 Adipokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100224&lng=en&tlng=en |
| work_keys_str_mv | AT petraborilovalinhartova adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT juliusjanos adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT hanaposkerova adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT denisakavrikova adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT antoninfassmann adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT ladislavdusek adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy AT lydieizakovicovaholla adipokinegenevariabilityandplasmalevelsinpatientswithchronicperiodontitisacasecontrolstudy |