Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining
Co-products of vegetable oil refining such as a mixed deodorizer distillate resulting from the refining of various vegetable oils, a crude distillate resulting from the physical refining of coconut oil and commercial mixtures of distilled sunflower and coconut fatty acids were used as starting mater...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2008-09-01
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| Series: | Grasas y Aceites |
| Subjects: | |
| Online Access: | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/515 |
| _version_ | 1831681871111520256 |
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| author | Kamol Tangkam Nikolaus Weber Berthold Wiege |
| author_facet | Kamol Tangkam Nikolaus Weber Berthold Wiege |
| author_sort | Kamol Tangkam |
| collection | DOAJ |
| description | Co-products of vegetable oil refining such as a mixed deodorizer distillate resulting from the refining of various vegetable oils, a crude distillate resulting from the physical refining of coconut oil and commercial mixtures of distilled sunflower and coconut fatty acids were used as starting materials for the enzymatic preparation of diglycerides. Reaction conditions (temperature, pressure, molar ratio) for the formation of diglycerides by lipase-catalyzed esterification/transesterification were studied using the mixed deodorizer distillate and glycerol as starting materials. The best results were obtained with the immobilized lipase B from Candida antarctica (Novozym 435) in vacuo at 60 °C leading to moderate proportions (~52%) of diglycerides. The proportion of diglycerides increased when residual acylglycerides of the co-products of vegetable oil refining were hydrolyzed prior to esterification. Thus, the esterification of hydrolyzed co-products of vegetable oil refining with glycerol led to high formation (62-72%) of diglycerides. Short-path vacuum distillation of the esterification products yielded distillation residues containing from 70% to 94% diglycerides. The proportions of fatty acids and monoglycerides in the distilled residues were quite low (≤ 1% and 1 to 3.9%, respectively). Immobilized lipases from Rhizomucor miehei and Thermomyces lanuginosus were less active as esterification biocatalysts. |
| first_indexed | 2024-12-20T06:07:55Z |
| format | Article |
| id | doaj.art-c76767364d6c412cbb320360bca7a033 |
| institution | Directory Open Access Journal |
| issn | 0017-3495 1988-4214 |
| language | English |
| last_indexed | 2024-12-20T06:07:55Z |
| publishDate | 2008-09-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Grasas y Aceites |
| spelling | doaj.art-c76767364d6c412cbb320360bca7a0332022-12-21T19:50:46ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142008-09-0159324525310.3989/gya.2008.v59.i3.515510Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refiningKamol Tangkam0Nikolaus Weber1Berthold Wiege2Max Rubner Institute, Federal Research Institute for Nutrition and FoodMax Rubner Institute, Federal Research Institute for Nutrition and FoodMax Rubner Institute, Federal Research Institute for Nutrition and FoodCo-products of vegetable oil refining such as a mixed deodorizer distillate resulting from the refining of various vegetable oils, a crude distillate resulting from the physical refining of coconut oil and commercial mixtures of distilled sunflower and coconut fatty acids were used as starting materials for the enzymatic preparation of diglycerides. Reaction conditions (temperature, pressure, molar ratio) for the formation of diglycerides by lipase-catalyzed esterification/transesterification were studied using the mixed deodorizer distillate and glycerol as starting materials. The best results were obtained with the immobilized lipase B from Candida antarctica (Novozym 435) in vacuo at 60 °C leading to moderate proportions (~52%) of diglycerides. The proportion of diglycerides increased when residual acylglycerides of the co-products of vegetable oil refining were hydrolyzed prior to esterification. Thus, the esterification of hydrolyzed co-products of vegetable oil refining with glycerol led to high formation (62-72%) of diglycerides. Short-path vacuum distillation of the esterification products yielded distillation residues containing from 70% to 94% diglycerides. The proportions of fatty acids and monoglycerides in the distilled residues were quite low (≤ 1% and 1 to 3.9%, respectively). Immobilized lipases from Rhizomucor miehei and Thermomyces lanuginosus were less active as esterification biocatalysts.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/515co-products of vegetable oil refiningdiglyceridesdistilled fatty acidsesterificationimmobilized microbial lipasestransesterification |
| spellingShingle | Kamol Tangkam Nikolaus Weber Berthold Wiege Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining Grasas y Aceites co-products of vegetable oil refining diglycerides distilled fatty acids esterification immobilized microbial lipases transesterification |
| title | Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining |
| title_full | Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining |
| title_fullStr | Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining |
| title_full_unstemmed | Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining |
| title_short | Solvent-free lipase-catalyzed preparation of diglycerides from co-products of vegetable oil refining |
| title_sort | solvent free lipase catalyzed preparation of diglycerides from co products of vegetable oil refining |
| topic | co-products of vegetable oil refining diglycerides distilled fatty acids esterification immobilized microbial lipases transesterification |
| url | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/515 |
| work_keys_str_mv | AT kamoltangkam solventfreelipasecatalyzedpreparationofdiglyceridesfromcoproductsofvegetableoilrefining AT nikolausweber solventfreelipasecatalyzedpreparationofdiglyceridesfromcoproductsofvegetableoilrefining AT bertholdwiege solventfreelipasecatalyzedpreparationofdiglyceridesfromcoproductsofvegetableoilrefining |