Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis
Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators—auxin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.830840/full |
| _version_ | 1811297854385291264 |
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| author | Ming Wang Ming Wang Laurent Ogé Maria-Dolores Pérez Garcia Alexandra Launay-Avon Gilles Clément Jose Le Gourrierec Latifa Hamama Soulaiman Sakr |
| author_facet | Ming Wang Ming Wang Laurent Ogé Maria-Dolores Pérez Garcia Alexandra Launay-Avon Gilles Clément Jose Le Gourrierec Latifa Hamama Soulaiman Sakr |
| author_sort | Ming Wang |
| collection | DOAJ |
| description | Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators—auxin and sugar—antagonistically regulate plant branching. However, little is known regarding the molecular mechanisms involved in this crosstalk. We carried out two complementary untargeted approaches—RNA-seq and metabolomics—on explant stem buds fed with different concentrations of auxin and sucrose resulting in dormant and non-dormant buds. Buds responded to the combined effect of auxin and sugar by massive reprogramming of the transcriptome and metabolome. The antagonistic effect of sucrose and auxin targeted several important physiological processes, including sink strength, the amino acid metabolism, the sulfate metabolism, ribosome biogenesis, the nucleic acid metabolism, and phytohormone signaling. Further experiments revealed a role of the TOR-kinase signaling pathway in bud outgrowth through at least downregulation of Rosa hybrida BRANCHED1 (RhBRC1). These new findings represent a cornerstone to further investigate the diverse molecular mechanisms that drive the integration of endogenous factors during shoot branching. |
| first_indexed | 2024-04-13T06:11:08Z |
| format | Article |
| id | doaj.art-c767879c76214b849b2c8e69e5ee7047 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T06:11:08Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c767879c76214b849b2c8e69e5ee70472022-12-22T02:59:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.830840830840Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics AnalysisMing Wang0Ming Wang1Laurent Ogé2Maria-Dolores Pérez Garcia3Alexandra Launay-Avon4Gilles Clément5Jose Le Gourrierec6Latifa Hamama7Soulaiman Sakr8Dryland-Technology Key Laboratory of Shandong Province, College of Agronomy, Qingdao Agricultural University, Qingdao, ChinaInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceInstitute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Université Paris-Sud, Université d’Evry, Université Paris-Saclay, Gif-sur-Yvette, FranceInstitut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, FranceInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceInstitut Agro, University of Angers INRAE, IRHS, SFR QUASAV, Angers, FranceShoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators—auxin and sugar—antagonistically regulate plant branching. However, little is known regarding the molecular mechanisms involved in this crosstalk. We carried out two complementary untargeted approaches—RNA-seq and metabolomics—on explant stem buds fed with different concentrations of auxin and sucrose resulting in dormant and non-dormant buds. Buds responded to the combined effect of auxin and sugar by massive reprogramming of the transcriptome and metabolome. The antagonistic effect of sucrose and auxin targeted several important physiological processes, including sink strength, the amino acid metabolism, the sulfate metabolism, ribosome biogenesis, the nucleic acid metabolism, and phytohormone signaling. Further experiments revealed a role of the TOR-kinase signaling pathway in bud outgrowth through at least downregulation of Rosa hybrida BRANCHED1 (RhBRC1). These new findings represent a cornerstone to further investigate the diverse molecular mechanisms that drive the integration of endogenous factors during shoot branching.https://www.frontiersin.org/articles/10.3389/fpls.2022.830840/fullbud outgrowthsucroseauxinsignaling pathwayTOR-kinase |
| spellingShingle | Ming Wang Ming Wang Laurent Ogé Maria-Dolores Pérez Garcia Alexandra Launay-Avon Gilles Clément Jose Le Gourrierec Latifa Hamama Soulaiman Sakr Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis Frontiers in Plant Science bud outgrowth sucrose auxin signaling pathway TOR-kinase |
| title | Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| title_full | Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| title_fullStr | Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| title_full_unstemmed | Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| title_short | Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| title_sort | antagonistic effect of sucrose availability and auxin on rosa axillary bud metabolism and signaling based on the transcriptomics and metabolomics analysis |
| topic | bud outgrowth sucrose auxin signaling pathway TOR-kinase |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.830840/full |
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