LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer
Epigenetic alteration is a key feature that contributes to the progression of bladder cancer (BC) and long non-coding RNAs serve crucial role in the epigenetic modulation. This study was designed to explore the epigenetic regulation of LINC00592 in BC. LINC00592 expression in BC was examined. Then,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-09-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2023-0788 |
| _version_ | 1797668793601753088 |
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| author | Wu Tieqiu Li Nannan Wu Xinghui Du Yongchao Tang Zhiwang |
| author_facet | Wu Tieqiu Li Nannan Wu Xinghui Du Yongchao Tang Zhiwang |
| author_sort | Wu Tieqiu |
| collection | DOAJ |
| description | Epigenetic alteration is a key feature that contributes to the progression of bladder cancer (BC) and long non-coding RNAs serve crucial role in the epigenetic modulation. This study was designed to explore the epigenetic regulation of LINC00592 in BC. LINC00592 expression in BC was examined. Then, LINC00592 was silenced in BC cell followed by cell behavior analyses using CCK-8, transwell, western blot, or flow cytometry. Potential downstream target of LINC00592 was explored using RNA pull-down assay and methylation of WIF1 was determined using methylated-specific PCR. In addition, WIF1 or/and LINC00592 were silenced in BC cells followed by cell behavior analyses to explore the regulation between them. Upregulation of LINC00592 was significantly detected in BC tissues and cells. In BC cells silencing LINC00592 suppressed the proliferation, migration, and epithelial-mesenchymal transitions (EMT), but enhanced apoptosis. Moreover, LINC00592 recruited DNMT1, DNMT3A, and DNMT3B to enhance WIF1 promoter methylation. In addition, WIF1 overexpression suppressed the proliferation, migration, as well as EMT, but enhanced apoptosis. Silencing WIF1 significantly attenuated the role of silencing LINC00592 in suppressing the proliferative, migratory, and EMT ability of BC cells, and increasing the apoptosis. LINC00592 promoted the growth and metastasis of BC via enhancing the promoter methylation of WIF1 and decreasing WIF1 transcription. |
| first_indexed | 2024-03-11T20:34:39Z |
| format | Article |
| id | doaj.art-c7692453d55240a9a2fd51b370b4fd64 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-03-11T20:34:39Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-c7692453d55240a9a2fd51b370b4fd642023-10-02T07:38:17ZengDe GruyterOpen Medicine2391-54632023-09-0118119809110.1515/med-2023-0788LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancerWu Tieqiu0Li Nannan1Wu Xinghui2Du Yongchao3Tang Zhiwang4Department of Urology, The First Hospital of Changsha, Changsha, Hunan, PR ChinaDepartment of Urology, The First Hospital of Changsha, Changsha, Hunan, PR ChinaDepartment of Urology, The First Hospital of Changsha, Changsha, Hunan, PR ChinaDepartment of Urology, The First Hospital of Changsha, Changsha, Hunan, PR ChinaDepartment of Urology, The First Hospital of Changsha, No. 311 Yingpan Road, Kaifu District, Changsha, Hunan, PR ChinaEpigenetic alteration is a key feature that contributes to the progression of bladder cancer (BC) and long non-coding RNAs serve crucial role in the epigenetic modulation. This study was designed to explore the epigenetic regulation of LINC00592 in BC. LINC00592 expression in BC was examined. Then, LINC00592 was silenced in BC cell followed by cell behavior analyses using CCK-8, transwell, western blot, or flow cytometry. Potential downstream target of LINC00592 was explored using RNA pull-down assay and methylation of WIF1 was determined using methylated-specific PCR. In addition, WIF1 or/and LINC00592 were silenced in BC cells followed by cell behavior analyses to explore the regulation between them. Upregulation of LINC00592 was significantly detected in BC tissues and cells. In BC cells silencing LINC00592 suppressed the proliferation, migration, and epithelial-mesenchymal transitions (EMT), but enhanced apoptosis. Moreover, LINC00592 recruited DNMT1, DNMT3A, and DNMT3B to enhance WIF1 promoter methylation. In addition, WIF1 overexpression suppressed the proliferation, migration, as well as EMT, but enhanced apoptosis. Silencing WIF1 significantly attenuated the role of silencing LINC00592 in suppressing the proliferative, migratory, and EMT ability of BC cells, and increasing the apoptosis. LINC00592 promoted the growth and metastasis of BC via enhancing the promoter methylation of WIF1 and decreasing WIF1 transcription.https://doi.org/10.1515/med-2023-0788bladder cancerlinc00592wif1promotermethylation |
| spellingShingle | Wu Tieqiu Li Nannan Wu Xinghui Du Yongchao Tang Zhiwang LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer Open Medicine bladder cancer linc00592 wif1 promoter methylation |
| title | LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer |
| title_full | LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer |
| title_fullStr | LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer |
| title_full_unstemmed | LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer |
| title_short | LncRNA LINC00592 mediates the promoter methylation of WIF1 to promote the development of bladder cancer |
| title_sort | lncrna linc00592 mediates the promoter methylation of wif1 to promote the development of bladder cancer |
| topic | bladder cancer linc00592 wif1 promoter methylation |
| url | https://doi.org/10.1515/med-2023-0788 |
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