Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood
Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and par...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-03-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844017337052 |
| _version_ | 1818907087176990720 |
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| author | Nguyen Bao Quoc Nguyen Doan Nguyen Phuong Nguyen Ngoc Bao Chau Do Thi Phuong Linh |
| author_facet | Nguyen Bao Quoc Nguyen Doan Nguyen Phuong Nguyen Ngoc Bao Chau Do Thi Phuong Linh |
| author_sort | Nguyen Bao Quoc |
| collection | DOAJ |
| description | Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and portable real-time fluorometer. The final optimized fluorescence-based LAMP assay provided significant amplification time of less than 15 minutes compared with over 1 hour for PCR and an opened tube LAMP system described previously. Results indicated that fluorescence-based LAMP assay was more sensitive than PCR as a rapid, sensitive, efficient, and highly reliable approach for rapid detection of HBV. |
| first_indexed | 2024-12-19T21:49:32Z |
| format | Article |
| id | doaj.art-c769f3d6417f4d748dcb6c40ad65f9de |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T21:49:32Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c769f3d6417f4d748dcb6c40ad65f9de2022-12-21T20:04:27ZengElsevierHeliyon2405-84402018-03-0143e00561Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human bloodNguyen Bao Quoc0Nguyen Doan Nguyen Phuong1Nguyen Ngoc Bao Chau2Do Thi Phuong Linh3Research Institute for Biotechnology and Environment, Nong Lam University, Ho Chi Minh City, Vietnam; Corresponding author.Research Institute for Biotechnology and Environment, Nong Lam University, Ho Chi Minh City, VietnamFaculty of Biotechnology, Ho Chi Minh City Open University, VietnamSpecialized Medical Center, National Institute of Malariology, Parasitology and Entomology, Ho Chi Minh City, VietnamRecently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and portable real-time fluorometer. The final optimized fluorescence-based LAMP assay provided significant amplification time of less than 15 minutes compared with over 1 hour for PCR and an opened tube LAMP system described previously. Results indicated that fluorescence-based LAMP assay was more sensitive than PCR as a rapid, sensitive, efficient, and highly reliable approach for rapid detection of HBV.http://www.sciencedirect.com/science/article/pii/S2405844017337052MedicineEvidence-based medicineInfectious diseasePathologyBiochemistryBioengineering |
| spellingShingle | Nguyen Bao Quoc Nguyen Doan Nguyen Phuong Nguyen Ngoc Bao Chau Do Thi Phuong Linh Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood Heliyon Medicine Evidence-based medicine Infectious disease Pathology Biochemistry Bioengineering |
| title | Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood |
| title_full | Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood |
| title_fullStr | Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood |
| title_full_unstemmed | Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood |
| title_short | Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood |
| title_sort | closed tube loop mediated isothermal amplification assay for rapid detection of hepatitis b virus in human blood |
| topic | Medicine Evidence-based medicine Infectious disease Pathology Biochemistry Bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2405844017337052 |
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