Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
Two independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by...
| मुख्य लेखकों: | , , , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
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MDPI AG
2019-01-01
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| श्रृंखला: | Brain Sciences |
| विषय: | |
| ऑनलाइन पहुंच: | http://www.mdpi.com/2076-3425/9/1/2 |
| _version_ | 1828485107723272192 |
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| author | John C. Crabbe Pamela Metten Antonia M. Savarese Angela R. Ozburn Jason P. Schlumbohm Stephanie E. Spence Wyatt R. Hack |
| author_facet | John C. Crabbe Pamela Metten Antonia M. Savarese Angela R. Ozburn Jason P. Schlumbohm Stephanie E. Spence Wyatt R. Hack |
| author_sort | John C. Crabbe |
| collection | DOAJ |
| description | Two independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by ethanol injections as compared with their unselected HS/NPT founder stock. We have subsequently developed inbred variants of each line. The current experiments established that reduced ethanol-conditioned taste aversion is also seen in the inbred variants, in both males and females. In other experiments, we asked whether HDID mice would ingest sufficient doses of ethanol to lead to a conditioned taste aversion upon retest. Different manipulations were used to elevate consumption of ethanol on initial exposure. Access to increased ethanol concentrations, to multiple tubes of ethanol, and fluid restriction to increase thirst motivation all enhanced initial drinking of ethanol. Each condition led to reduced intake the next day, consistent with a mild conditioned taste aversion. These experiments support the conclusion that one reason contributing to the willingness of HDID mice to drink to the point of intoxication is a genetic insensitivity to the aversive effects of ethanol. |
| first_indexed | 2024-12-11T09:08:09Z |
| format | Article |
| id | doaj.art-c76b96e7c61040148f41afe1a2e5fb6f |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-12-11T09:08:09Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-c76b96e7c61040148f41afe1a2e5fb6f2022-12-22T01:13:35ZengMDPI AGBrain Sciences2076-34252019-01-0191210.3390/brainsci9010002brainsci9010002Ethanol Conditioned Taste Aversion in High Drinking in the Dark MiceJohn C. Crabbe0Pamela Metten1Antonia M. Savarese2Angela R. Ozburn3Jason P. Schlumbohm4Stephanie E. Spence5Wyatt R. Hack6Portland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USAPortland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University and VA Portland Health Care System, Portland, OR 97239, USATwo independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by ethanol injections as compared with their unselected HS/NPT founder stock. We have subsequently developed inbred variants of each line. The current experiments established that reduced ethanol-conditioned taste aversion is also seen in the inbred variants, in both males and females. In other experiments, we asked whether HDID mice would ingest sufficient doses of ethanol to lead to a conditioned taste aversion upon retest. Different manipulations were used to elevate consumption of ethanol on initial exposure. Access to increased ethanol concentrations, to multiple tubes of ethanol, and fluid restriction to increase thirst motivation all enhanced initial drinking of ethanol. Each condition led to reduced intake the next day, consistent with a mild conditioned taste aversion. These experiments support the conclusion that one reason contributing to the willingness of HDID mice to drink to the point of intoxication is a genetic insensitivity to the aversive effects of ethanol.http://www.mdpi.com/2076-3425/9/1/2binge drinkingaversionselective breedingpharmacogeneticsinbred strainsavailabilityconcentrationthirst motivationanimal model |
| spellingShingle | John C. Crabbe Pamela Metten Antonia M. Savarese Angela R. Ozburn Jason P. Schlumbohm Stephanie E. Spence Wyatt R. Hack Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice Brain Sciences binge drinking aversion selective breeding pharmacogenetics inbred strains availability concentration thirst motivation animal model |
| title | Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice |
| title_full | Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice |
| title_fullStr | Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice |
| title_full_unstemmed | Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice |
| title_short | Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice |
| title_sort | ethanol conditioned taste aversion in high drinking in the dark mice |
| topic | binge drinking aversion selective breeding pharmacogenetics inbred strains availability concentration thirst motivation animal model |
| url | http://www.mdpi.com/2076-3425/9/1/2 |
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