Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>)
We used genotyping-by-sequencing (GBS) to identify sex-linked markers in 43 wild-collected spiny frog (<i>Quasipaa boulengeri</i>) adults from a single site. We identified a total of 1049 putatively sex-linked GBS-tags, 98% of which indicated an XX/XY system, and finally confirmed 574 XY...
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| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/4/575 |
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| author | Xusheng Yang Wei Luo Yun Xia Xiaomao Zeng |
| author_facet | Xusheng Yang Wei Luo Yun Xia Xiaomao Zeng |
| author_sort | Xusheng Yang |
| collection | DOAJ |
| description | We used genotyping-by-sequencing (GBS) to identify sex-linked markers in 43 wild-collected spiny frog (<i>Quasipaa boulengeri</i>) adults from a single site. We identified a total of 1049 putatively sex-linked GBS-tags, 98% of which indicated an XX/XY system, and finally confirmed 574 XY-type sex-linked loci. The sex specificity of five markers was further validated by PCR amplification using a large number of additional individuals from 26 populations of this species. A total of 27 sex linkage markers matched with the <i>Dmrt1</i> gene, showing a conserved role in sex determination and differentiation in different organisms from flies and nematodes to mammals. Chromosome 1, which harbors <i>Dmrt1</i>, was considered as the most likely candidate sex chromosome in anurans. Five sex-linked SNP makers indicated sex reversals, which are sparsely present in wild amphibian populations, in three out of the one-hundred and thirty-three explored individuals. The variety of sex-linked markers identified could be used in population genetics analyses requiring information on individual sex or in investigations aimed at drawing inferences about sex determination and sex chromosome evolution. |
| first_indexed | 2024-03-09T10:36:39Z |
| format | Article |
| id | doaj.art-c76d8460c1424d7183d5b5885672714d |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:36:39Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-c76d8460c1424d7183d5b5885672714d2023-12-01T20:56:25ZengMDPI AGGenes2073-44252022-03-0113457510.3390/genes13040575Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>)Xusheng Yang0Wei Luo1Yun Xia2Xiaomao Zeng3Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaEcological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaWe used genotyping-by-sequencing (GBS) to identify sex-linked markers in 43 wild-collected spiny frog (<i>Quasipaa boulengeri</i>) adults from a single site. We identified a total of 1049 putatively sex-linked GBS-tags, 98% of which indicated an XX/XY system, and finally confirmed 574 XY-type sex-linked loci. The sex specificity of five markers was further validated by PCR amplification using a large number of additional individuals from 26 populations of this species. A total of 27 sex linkage markers matched with the <i>Dmrt1</i> gene, showing a conserved role in sex determination and differentiation in different organisms from flies and nematodes to mammals. Chromosome 1, which harbors <i>Dmrt1</i>, was considered as the most likely candidate sex chromosome in anurans. Five sex-linked SNP makers indicated sex reversals, which are sparsely present in wild amphibian populations, in three out of the one-hundred and thirty-three explored individuals. The variety of sex-linked markers identified could be used in population genetics analyses requiring information on individual sex or in investigations aimed at drawing inferences about sex determination and sex chromosome evolution.https://www.mdpi.com/2073-4425/13/4/575GBSamphibiangenomesex reversal |
| spellingShingle | Xusheng Yang Wei Luo Yun Xia Xiaomao Zeng Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) Genes GBS amphibian genome sex reversal |
| title | Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) |
| title_full | Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) |
| title_fullStr | Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) |
| title_full_unstemmed | Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) |
| title_short | Using Sex-Linked Markers via Genotyping-by-Sequencing to Identify XX/XY Sex Chromosomes in the Spiny Frog (<i>Quasipaa boulengeri</i>) |
| title_sort | using sex linked markers via genotyping by sequencing to identify xx xy sex chromosomes in the spiny frog i quasipaa boulengeri i |
| topic | GBS amphibian genome sex reversal |
| url | https://www.mdpi.com/2073-4425/13/4/575 |
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