In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid
The <i>Cattleya</i> (Orchidaceae–<i>Laeliinae</i> subtribe) intergeneric hybrids, such as <i>Brassolaeliocattleya</i> (<i>Blc</i>.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial indu...
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/2/281 |
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| author | Joe Abdul Vilcherrez-Atoche Jéssica Coutinho Silva Wellington Ronildo Clarindo Mateus Mondin Jean Carlos Cardoso |
| author_facet | Joe Abdul Vilcherrez-Atoche Jéssica Coutinho Silva Wellington Ronildo Clarindo Mateus Mondin Jean Carlos Cardoso |
| author_sort | Joe Abdul Vilcherrez-Atoche |
| collection | DOAJ |
| description | The <i>Cattleya</i> (Orchidaceae–<i>Laeliinae</i> subtribe) intergeneric hybrids, such as <i>Brassolaeliocattleya</i> (<i>Blc</i>.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to plants. Polyploidization efficiency depends on factors, such as the type of antimitotic, polyploidization method, concentrations, exposure times and type of explant. This study aimed to develop a protocol to polyploidize <i>Blc</i>. orchids, by testing two types of explants (seeds and protocorms), concentrations and exposure times to colchicine. The effects of colchicine on the in vitro development of explants were also investigated. The responses of explants to colchicine depended on the concentrations, exposure time and the interaction of these factors. Flow cytometric analysis evidenced high endopolyploidy and allowed the separation of polyploidized (4C, 8C and 16C peaks) from non-polyploidized (only 2C and 4C peaks) plants. The highest percentage of polyploid plants was regenerated from protocorms (16.4%) treated with colchicine instead of seeds (3.2%). Protocorms treated with colchicine at 500–750 μM for 18 h resulted in the best percentage of polyploidization. Additionally, in vitro natural polyploidization using protocorms was reported (11.5%). Cytological analyses allowed an estimation of the number of chromosomes of the parents (≡70), polyploidized (≡140) and non-polyploidized progeny (≡70). |
| first_indexed | 2024-03-09T11:25:54Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:25:54Z |
| publishDate | 2023-01-01 |
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| series | Plants |
| spelling | doaj.art-c76eb696e2f54806b050342fa8bbfa7f2023-12-01T00:03:59ZengMDPI AGPlants2223-77472023-01-0112228110.3390/plants12020281In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid OrchidJoe Abdul Vilcherrez-Atoche0Jéssica Coutinho Silva1Wellington Ronildo Clarindo2Mateus Mondin3Jean Carlos Cardoso4Laboratory of Plant Physiology and Tissue Culture, Depart of Biotechnology, Plant and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos (CCA/UFSCar), Rodovia Anhanguera, km 174, CP 153, Araras 13600-970, SP, BrazilLaboratório de Citogenética e Citometria, Departamento de Biologia Geral, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa 36570-000, MG, BrazilLaboratório de Citogenética e Citometria, Departamento de Biologia Geral, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Viçosa 36570-000, MG, BrazilDepartamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba 13400-970, SP, BrazilLaboratory of Plant Physiology and Tissue Culture, Depart of Biotechnology, Plant and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos (CCA/UFSCar), Rodovia Anhanguera, km 174, CP 153, Araras 13600-970, SP, BrazilThe <i>Cattleya</i> (Orchidaceae–<i>Laeliinae</i> subtribe) intergeneric hybrids, such as <i>Brassolaeliocattleya</i> (<i>Blc</i>.), have great ornamental value, due to their compact-size, with large and high color diversity of flowers. Artificial induction of polyploidy brings agronomic, ornamental and genetic benefits to plants. Polyploidization efficiency depends on factors, such as the type of antimitotic, polyploidization method, concentrations, exposure times and type of explant. This study aimed to develop a protocol to polyploidize <i>Blc</i>. orchids, by testing two types of explants (seeds and protocorms), concentrations and exposure times to colchicine. The effects of colchicine on the in vitro development of explants were also investigated. The responses of explants to colchicine depended on the concentrations, exposure time and the interaction of these factors. Flow cytometric analysis evidenced high endopolyploidy and allowed the separation of polyploidized (4C, 8C and 16C peaks) from non-polyploidized (only 2C and 4C peaks) plants. The highest percentage of polyploid plants was regenerated from protocorms (16.4%) treated with colchicine instead of seeds (3.2%). Protocorms treated with colchicine at 500–750 μM for 18 h resulted in the best percentage of polyploidization. Additionally, in vitro natural polyploidization using protocorms was reported (11.5%). Cytological analyses allowed an estimation of the number of chromosomes of the parents (≡70), polyploidized (≡140) and non-polyploidized progeny (≡70).https://www.mdpi.com/2223-7747/12/2/281orchid hybridizationin vitro cultureseedlingcolchicinepolyploidsflow cytometry |
| spellingShingle | Joe Abdul Vilcherrez-Atoche Jéssica Coutinho Silva Wellington Ronildo Clarindo Mateus Mondin Jean Carlos Cardoso In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid Plants orchid hybridization in vitro culture seedling colchicine polyploids flow cytometry |
| title | In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid |
| title_full | In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid |
| title_fullStr | In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid |
| title_full_unstemmed | In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid |
| title_short | In Vitro Polyploidization of <i>Brassolaeliocattleya</i> Hybrid Orchid |
| title_sort | in vitro polyploidization of i brassolaeliocattleya i hybrid orchid |
| topic | orchid hybridization in vitro culture seedling colchicine polyploids flow cytometry |
| url | https://www.mdpi.com/2223-7747/12/2/281 |
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