A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population
The incidence of colorectal cancer (CRC) is increasing, and currently it is the third most common cancer. Early CRC diagnosis is still difficult and relies on an invasive colonoscopy and tissue biopsy. The globally observed tendency demands non-invasive, specific, and accurate diagnostic tools for e...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/3/321 |
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| author | Oihane E. Albóniga Joaquín Cubiella Luis Bujanda María Encarnación Blanco Borja Lanza Cristina Alonso Beatriz Nafría Juan Manuel Falcón-Pérez |
| author_facet | Oihane E. Albóniga Joaquín Cubiella Luis Bujanda María Encarnación Blanco Borja Lanza Cristina Alonso Beatriz Nafría Juan Manuel Falcón-Pérez |
| author_sort | Oihane E. Albóniga |
| collection | DOAJ |
| description | The incidence of colorectal cancer (CRC) is increasing, and currently it is the third most common cancer. Early CRC diagnosis is still difficult and relies on an invasive colonoscopy and tissue biopsy. The globally observed tendency demands non-invasive, specific, and accurate diagnostic tools for early diagnosis and prognosis. In this work, the main aim was to evaluate for the first time the feasibility of using extracts from the non-invasive sample collection from faecal occult blood (FOB) kits for its use in metabolomics studies taking advantage in this way of the high sensitivity of this technology. Then, a cohort of 131 samples from control individuals (CTL), adenoma (AD) and CRC patients were analysed using a semitargeted approach by ultra-high-performance liquid chromatography–time-of-flight–mass spectrometry (UHPLC-ToF-MS). Multivariate and univariate statistical analysis revealed that cholesteryl esters (ChoE) with polyunsaturated fatty acids (PUFAs) together with FOB were relevant metabolites that could clearly separate CRC patients from AD and CTL individuals, whereas the metabolic profiles of CTL and AD were very similar. These results are in agreement with previous findings and reveal the advantage of using the same FOBT samples for several analyses, which would facilitate sample collection and improve direct connection between FOB measurements and metabolomics analysis. Although the sample size and the number of metabolites should be enhanced to cover a wider range of metabolites, alterations in lipid metabolism clearly point out for future perspectives. |
| first_indexed | 2024-03-11T06:11:39Z |
| format | Article |
| id | doaj.art-c7712c4842124a52aa2199765e9da826 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T06:11:39Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-c7712c4842124a52aa2199765e9da8262023-11-17T12:35:52ZengMDPI AGMetabolites2218-19892023-02-0113332110.3390/metabo13030321A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer PopulationOihane E. Albóniga0Joaquín Cubiella1Luis Bujanda2María Encarnación Blanco3Borja Lanza4Cristina Alonso5Beatriz Nafría6Juan Manuel Falcón-Pérez7Metabolomics Platform, CICbioGUNE-BRTA, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Bizkaia Technology Park, 48160 Derio, SpainDepartment of Gastroenterology, Instituto de Investigación Sanitaria Galicia Sur, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Complexo Hospitalario Universitario de Ourense, 32005 Ourense, SpainDepartment of Gastroenterology, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Biodonostia Health Research Institute, Donosti Hospital, University of the Basque Country (UPV/EHU), 20014 San Sebastián, SpainOWL Metabolomics, Bizkaia Technology Park, 48160 Derio, SpainOWL Metabolomics, Bizkaia Technology Park, 48160 Derio, SpainOWL Metabolomics, Bizkaia Technology Park, 48160 Derio, SpainClinical Biochemistry Department, Biodonostia Health Research Institute Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation, 20014 San Sebastian, SpainMetabolomics Platform, CICbioGUNE-BRTA, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Bizkaia Technology Park, 48160 Derio, SpainThe incidence of colorectal cancer (CRC) is increasing, and currently it is the third most common cancer. Early CRC diagnosis is still difficult and relies on an invasive colonoscopy and tissue biopsy. The globally observed tendency demands non-invasive, specific, and accurate diagnostic tools for early diagnosis and prognosis. In this work, the main aim was to evaluate for the first time the feasibility of using extracts from the non-invasive sample collection from faecal occult blood (FOB) kits for its use in metabolomics studies taking advantage in this way of the high sensitivity of this technology. Then, a cohort of 131 samples from control individuals (CTL), adenoma (AD) and CRC patients were analysed using a semitargeted approach by ultra-high-performance liquid chromatography–time-of-flight–mass spectrometry (UHPLC-ToF-MS). Multivariate and univariate statistical analysis revealed that cholesteryl esters (ChoE) with polyunsaturated fatty acids (PUFAs) together with FOB were relevant metabolites that could clearly separate CRC patients from AD and CTL individuals, whereas the metabolic profiles of CTL and AD were very similar. These results are in agreement with previous findings and reveal the advantage of using the same FOBT samples for several analyses, which would facilitate sample collection and improve direct connection between FOB measurements and metabolomics analysis. Although the sample size and the number of metabolites should be enhanced to cover a wider range of metabolites, alterations in lipid metabolism clearly point out for future perspectives.https://www.mdpi.com/2218-1989/13/3/321cholesteryl esterscolorectal cancersemitargeted metabolomicslipidsFOB screening kits |
| spellingShingle | Oihane E. Albóniga Joaquín Cubiella Luis Bujanda María Encarnación Blanco Borja Lanza Cristina Alonso Beatriz Nafría Juan Manuel Falcón-Pérez A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population Metabolites cholesteryl esters colorectal cancer semitargeted metabolomics lipids FOB screening kits |
| title | A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population |
| title_full | A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population |
| title_fullStr | A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population |
| title_full_unstemmed | A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population |
| title_short | A Novel Approach on the Use of Samples from Faecal Occult Blood Screening Kits for Metabolomics Analysis: Application in Colorectal Cancer Population |
| title_sort | novel approach on the use of samples from faecal occult blood screening kits for metabolomics analysis application in colorectal cancer population |
| topic | cholesteryl esters colorectal cancer semitargeted metabolomics lipids FOB screening kits |
| url | https://www.mdpi.com/2218-1989/13/3/321 |
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