Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models
Introduction: Pain is managed using conventional drugs like paracetamol, aspirin and diclofenac among others. Synthetic drugs have many side effects. This study aimed at evaluating the analgesic potential of the dichloromethane leaf extracts of Eucalyptus globulus and Senna didymobotrya in mice. Met...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahrekord University of Medical Sciences
2020-10-01
|
| Series: | Journal of HerbMed Pharmacology |
| Subjects: | |
| Online Access: | http://herbmedpharmacol.com/PDF/jhp-9-391.pdf |
| _version_ | 1818199439919022080 |
|---|---|
| author | Joseph Kiambi Mworia Cromwell Mwiti Kibiti Joseph JN Ngeranwa Mathew Piero Ngugi |
| author_facet | Joseph Kiambi Mworia Cromwell Mwiti Kibiti Joseph JN Ngeranwa Mathew Piero Ngugi |
| author_sort | Joseph Kiambi Mworia |
| collection | DOAJ |
| description | Introduction: Pain is managed using conventional drugs like paracetamol, aspirin and diclofenac among others. Synthetic drugs have many side effects. This study aimed at evaluating the analgesic potential of the dichloromethane leaf extracts of Eucalyptus globulus and Senna didymobotrya in mice. Methods: The dichloromethane leaf extracts of E. globulus and S. didymobotrya were subjected to quantitative phytochemical analysis using gas chromatography-mass spectrophotometry (GC-MS). In vivo analgesic evaluation comprised of nine groups of animals (Swiss albino mice): normal, positive, negative control and six experimental groups that received 25, 50, 100, 150, 200 and 250 mg/kg body weight of each plant extract intraperitoneally. Thirty minutes later, they were injected with 0.01 mL of 2.5% formalin. The animals in positive control group were administered diclofenac (15 mg/kg) and formalin, the normal control mice received 3% dimethyl sulfoxide (DMSO) in normal saline, while the negative group received DMSO in normal saline and formalin. All the doses were administered intraperitoneally. The duration of shaking and licking of the injected paw was scored and analyzed. Results: The analysis revealed that E. globulus contained alpha-pinenes, endo-fenchol, α-eudesmol, myrcene, camphene, alpha-phellandrene, limonene, and camphor while S. dymobotrya possessed camphene, alpha-phellandrene, limonene, and camphor. In the late phase, E. globulus at the doses of 25, 50, 100, 150, 200 and 250 mg/kg reduced the paw licking time by 34.03%, 60.79%, 84.33 %, 90.65%, 94.49%, 98.52%, respectively while S. didymobotrya extract reduced the paw licking time by 26.48%, 32.96%, 87.04%, 91.27%, 93.40%, 90.97%, and 96.82%, respectively. Conclusion: The results of this study validate and support the traditional uses of these plants as analgesics. |
| first_indexed | 2024-12-12T02:21:47Z |
| format | Article |
| id | doaj.art-c773ef120e9942d48fb16465f20c6531 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-12T02:21:47Z |
| publishDate | 2020-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-c773ef120e9942d48fb16465f20c65312022-12-22T00:41:40ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042020-10-019439139910.34172/jhp.2020.49jhp-36637Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice modelsJoseph Kiambi Mworia0Cromwell Mwiti Kibiti1Joseph JN Ngeranwa2Mathew Piero Ngugi3Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, P. O Box 43844-00100, Nairobi, KenyaDepartment of Pure and Applied Sciences, Technical University of Mombasa, P.O Box 90420-80100, Mombasa, KenyaDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, P. O Box 43844-00100, Nairobi, KenyaDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, P. O Box 43844-00100, Nairobi, KenyaIntroduction: Pain is managed using conventional drugs like paracetamol, aspirin and diclofenac among others. Synthetic drugs have many side effects. This study aimed at evaluating the analgesic potential of the dichloromethane leaf extracts of Eucalyptus globulus and Senna didymobotrya in mice. Methods: The dichloromethane leaf extracts of E. globulus and S. didymobotrya were subjected to quantitative phytochemical analysis using gas chromatography-mass spectrophotometry (GC-MS). In vivo analgesic evaluation comprised of nine groups of animals (Swiss albino mice): normal, positive, negative control and six experimental groups that received 25, 50, 100, 150, 200 and 250 mg/kg body weight of each plant extract intraperitoneally. Thirty minutes later, they were injected with 0.01 mL of 2.5% formalin. The animals in positive control group were administered diclofenac (15 mg/kg) and formalin, the normal control mice received 3% dimethyl sulfoxide (DMSO) in normal saline, while the negative group received DMSO in normal saline and formalin. All the doses were administered intraperitoneally. The duration of shaking and licking of the injected paw was scored and analyzed. Results: The analysis revealed that E. globulus contained alpha-pinenes, endo-fenchol, α-eudesmol, myrcene, camphene, alpha-phellandrene, limonene, and camphor while S. dymobotrya possessed camphene, alpha-phellandrene, limonene, and camphor. In the late phase, E. globulus at the doses of 25, 50, 100, 150, 200 and 250 mg/kg reduced the paw licking time by 34.03%, 60.79%, 84.33 %, 90.65%, 94.49%, 98.52%, respectively while S. didymobotrya extract reduced the paw licking time by 26.48%, 32.96%, 87.04%, 91.27%, 93.40%, 90.97%, and 96.82%, respectively. Conclusion: The results of this study validate and support the traditional uses of these plants as analgesics.http://herbmedpharmacol.com/PDF/jhp-9-391.pdfeucalyptus globulussenna didymobtryapainphytochemicalsformalin test |
| spellingShingle | Joseph Kiambi Mworia Cromwell Mwiti Kibiti Joseph JN Ngeranwa Mathew Piero Ngugi Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models Journal of HerbMed Pharmacology eucalyptus globulus senna didymobtrya pain phytochemicals formalin test |
| title | Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models |
| title_full | Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models |
| title_fullStr | Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models |
| title_full_unstemmed | Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models |
| title_short | Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models |
| title_sort | analgesic potential of dichloromethane leaf extracts of eucalyptus globulus labill and senna didymobotrya fresenius in mice models |
| topic | eucalyptus globulus senna didymobtrya pain phytochemicals formalin test |
| url | http://herbmedpharmacol.com/PDF/jhp-9-391.pdf |
| work_keys_str_mv | AT josephkiambimworia analgesicpotentialofdichloromethaneleafextractsofeucalyptusglobuluslabillandsennadidymobotryafreseniusinmicemodels AT cromwellmwitikibiti analgesicpotentialofdichloromethaneleafextractsofeucalyptusglobuluslabillandsennadidymobotryafreseniusinmicemodels AT josephjnngeranwa analgesicpotentialofdichloromethaneleafextractsofeucalyptusglobuluslabillandsennadidymobotryafreseniusinmicemodels AT mathewpierongugi analgesicpotentialofdichloromethaneleafextractsofeucalyptusglobuluslabillandsennadidymobotryafreseniusinmicemodels |