Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells
The integration of fluorescence and plasmonic properties into one molecule is of importance in developing multifunctional imaging and therapy nanoprobes. The aim of this research was to evaluate the fluorescent properties and the plasmonic–photothermal, therapeutic, and radiotherapeutic potential of...
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| Format: | Article |
| Language: | English |
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SAGE Publications
2017-05-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012117704768 |
| _version_ | 1797283973310709760 |
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| author | Héctor Mendoza-Nava PhD Guillermina Ferro-Flores PhD Flor de María Ramírez PhD Blanca Ocampo-García PhD Clara Santos-Cuevas PhD Erika Azorín-Vega PhD Nallely Jiménez-Mancilla PhD Myrna Luna-Gutiérrez PhD Keila Isaac-Olivé PhD |
| author_facet | Héctor Mendoza-Nava PhD Guillermina Ferro-Flores PhD Flor de María Ramírez PhD Blanca Ocampo-García PhD Clara Santos-Cuevas PhD Erika Azorín-Vega PhD Nallely Jiménez-Mancilla PhD Myrna Luna-Gutiérrez PhD Keila Isaac-Olivé PhD |
| author_sort | Héctor Mendoza-Nava PhD |
| collection | DOAJ |
| description | The integration of fluorescence and plasmonic properties into one molecule is of importance in developing multifunctional imaging and therapy nanoprobes. The aim of this research was to evaluate the fluorescent properties and the plasmonic–photothermal, therapeutic, and radiotherapeutic potential of 177 Lu–dendrimer conjugated to folate and bombesin with gold nanoparticles in the dendritic cavity ( 177 Lu–DenAuNP–folate–bombesin) when it is internalized in T47D breast cancer cells. The intense near-Infrared (NIR) fluorescence emitted at 825 nm from the conjugate inside cells corroborated the usefulness of DenAuNP–folate–bombesin for optical imaging. After laser irradiation, the presence of the nanosystem in cells caused a significant increase in the temperature of the medium (46.8°C, compared to 39.1°C without DenAuNP–folate–bombesin, P < 0.05), resulting in a significant decrease in cell viability (down to 16.51% ± 1.52%) due to the 177 Lu–DenAuNP–folate–bombesin plasmonic properties. After treatment with 177 Lu–DenAuNP–folate–bombesin, the T47D cell viability decreased 90% because of the radiation-absorbed dose (63.16 ± 4.20 Gy) delivered inside the cells. The 177 Lu–DenAuNP–folate–bombesin nanoprobe internalized in cancer cells exhibited properties suitable for optical imaging, plasmonic–photothermal therapy, and targeted radiotherapy. |
| first_indexed | 2024-03-07T17:39:22Z |
| format | Article |
| id | doaj.art-c7765a6bca7d477ebd56bfc2b93fdb80 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:39:22Z |
| publishDate | 2017-05-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-c7765a6bca7d477ebd56bfc2b93fdb802024-03-02T16:18:57ZengSAGE PublicationsMolecular Imaging1536-01212017-05-011610.1177/153601211770476810.1177_1536012117704768Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer CellsHéctor Mendoza-Nava PhD0Guillermina Ferro-Flores PhD1Flor de María Ramírez PhD2Blanca Ocampo-García PhD3Clara Santos-Cuevas PhD4Erika Azorín-Vega PhD5Nallely Jiménez-Mancilla PhD6Myrna Luna-Gutiérrez PhD7Keila Isaac-Olivé PhD8 Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, Mexico Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Departamento de Química, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico CONACyT, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Departamento de Materiales Radiactivos, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México, Mexico Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, MexicoThe integration of fluorescence and plasmonic properties into one molecule is of importance in developing multifunctional imaging and therapy nanoprobes. The aim of this research was to evaluate the fluorescent properties and the plasmonic–photothermal, therapeutic, and radiotherapeutic potential of 177 Lu–dendrimer conjugated to folate and bombesin with gold nanoparticles in the dendritic cavity ( 177 Lu–DenAuNP–folate–bombesin) when it is internalized in T47D breast cancer cells. The intense near-Infrared (NIR) fluorescence emitted at 825 nm from the conjugate inside cells corroborated the usefulness of DenAuNP–folate–bombesin for optical imaging. After laser irradiation, the presence of the nanosystem in cells caused a significant increase in the temperature of the medium (46.8°C, compared to 39.1°C without DenAuNP–folate–bombesin, P < 0.05), resulting in a significant decrease in cell viability (down to 16.51% ± 1.52%) due to the 177 Lu–DenAuNP–folate–bombesin plasmonic properties. After treatment with 177 Lu–DenAuNP–folate–bombesin, the T47D cell viability decreased 90% because of the radiation-absorbed dose (63.16 ± 4.20 Gy) delivered inside the cells. The 177 Lu–DenAuNP–folate–bombesin nanoprobe internalized in cancer cells exhibited properties suitable for optical imaging, plasmonic–photothermal therapy, and targeted radiotherapy.https://doi.org/10.1177/1536012117704768 |
| spellingShingle | Héctor Mendoza-Nava PhD Guillermina Ferro-Flores PhD Flor de María Ramírez PhD Blanca Ocampo-García PhD Clara Santos-Cuevas PhD Erika Azorín-Vega PhD Nallely Jiménez-Mancilla PhD Myrna Luna-Gutiérrez PhD Keila Isaac-Olivé PhD Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells Molecular Imaging |
| title | Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells |
| title_full | Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells |
| title_fullStr | Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells |
| title_full_unstemmed | Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells |
| title_short | Fluorescent, Plasmonic, and Radiotherapeutic Properties of the Lu–Dendrimer-AuNP–Folate–Bombesin Nanoprobe Located Inside Cancer Cells |
| title_sort | fluorescent plasmonic and radiotherapeutic properties of the lu dendrimer aunp folate bombesin nanoprobe located inside cancer cells |
| url | https://doi.org/10.1177/1536012117704768 |
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