The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae).
Bacterial and fungal infections induce a potent immune response in Drosophila melanogaster, but it is unclear whether viral infections induce an antiviral immune response. Using microarrays, we examined the changes in gene expression in Drosophila that occur in response to infection with the sigma v...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-08-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2730013?pdf=render |
| _version_ | 1818136505301860352 |
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| author | Jennifer Carpenter Stephan Hutter John F Baines Julia Roller Sarah S Saminadin-Peter John Parsch Francis M Jiggins |
| author_facet | Jennifer Carpenter Stephan Hutter John F Baines Julia Roller Sarah S Saminadin-Peter John Parsch Francis M Jiggins |
| author_sort | Jennifer Carpenter |
| collection | DOAJ |
| description | Bacterial and fungal infections induce a potent immune response in Drosophila melanogaster, but it is unclear whether viral infections induce an antiviral immune response. Using microarrays, we examined the changes in gene expression in Drosophila that occur in response to infection with the sigma virus, a negative-stranded RNA virus (Rhabdoviridae) that occurs in wild populations of D. melanogaster.We detected many changes in gene expression in infected flies, but found no evidence for the activation of the Toll, IMD or Jak-STAT pathways, which control immune responses against bacteria and fungi. We identified a number of functional categories of genes, including serine proteases, ribosomal proteins and chorion proteins that were overrepresented among the differentially expressed genes. We also found that the sigma virus alters the expression of many more genes in males than in females.These data suggest that either Drosophila do not mount an immune response against the sigma virus, or that the immune response is not controlled by known immune pathways. If the latter is true, the genes that we identified as differentially expressed after infection are promising candidates for controlling the host's response to the sigma virus. |
| first_indexed | 2024-12-11T09:41:28Z |
| format | Article |
| id | doaj.art-c778602324764cae9b647541a6191cef |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:41:28Z |
| publishDate | 2009-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c778602324764cae9b647541a6191cef2022-12-22T01:12:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-08-0148e683810.1371/journal.pone.0006838The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae).Jennifer CarpenterStephan HutterJohn F BainesJulia RollerSarah S Saminadin-PeterJohn ParschFrancis M JigginsBacterial and fungal infections induce a potent immune response in Drosophila melanogaster, but it is unclear whether viral infections induce an antiviral immune response. Using microarrays, we examined the changes in gene expression in Drosophila that occur in response to infection with the sigma virus, a negative-stranded RNA virus (Rhabdoviridae) that occurs in wild populations of D. melanogaster.We detected many changes in gene expression in infected flies, but found no evidence for the activation of the Toll, IMD or Jak-STAT pathways, which control immune responses against bacteria and fungi. We identified a number of functional categories of genes, including serine proteases, ribosomal proteins and chorion proteins that were overrepresented among the differentially expressed genes. We also found that the sigma virus alters the expression of many more genes in males than in females.These data suggest that either Drosophila do not mount an immune response against the sigma virus, or that the immune response is not controlled by known immune pathways. If the latter is true, the genes that we identified as differentially expressed after infection are promising candidates for controlling the host's response to the sigma virus.http://europepmc.org/articles/PMC2730013?pdf=render |
| spellingShingle | Jennifer Carpenter Stephan Hutter John F Baines Julia Roller Sarah S Saminadin-Peter John Parsch Francis M Jiggins The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). PLoS ONE |
| title | The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). |
| title_full | The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). |
| title_fullStr | The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). |
| title_full_unstemmed | The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). |
| title_short | The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). |
| title_sort | transcriptional response of drosophila melanogaster to infection with the sigma virus rhabdoviridae |
| url | http://europepmc.org/articles/PMC2730013?pdf=render |
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