Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer
<strong>Background:</strong> Numerous molecular changes are involved in the development and<br />progression of bladder cancer. Regular follow-up of patients is crucial due to the high<br />recurrence rate of bladder cancer. The aim of this study is to determine the role of&l...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-04-01
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| Series: | Middle East Journal of Cancer |
| Subjects: | |
| Online Access: | http://mejc.sums.ac.ir/article_44857_ca0e836ee29d928a78cbd757af6faf06.pdf |
| Summary: | <strong>Background:</strong> Numerous molecular changes are involved in the development and<br />progression of bladder cancer. Regular follow-up of patients is crucial due to the high<br />recurrence rate of bladder cancer. The aim of this study is to determine the role of<br />B-Raf proto-oncogene, serine/threonine kinase and ZEB2 expressions in onset and<br />progression of bladder cancer. We have also investigated their relationships to<br />pathological characteristics.<br /><strong>Methods:</strong> We conducted this case-control study on bladder cancer and its healthy<br />adjacent tissue, and normal bladder tissue from patients with benign prostatic hyperplasia.<br />After extraction of total RNA and cDNA synthesis, quantitative expression analysis was<br />performed in duplicate using real-time PCR. Changes in the gene expression were<br />calculated according to the 2(-ΔΔCt) equation. The products were confirmed by 1% agarose<br />gel electrophoresis and sequenced by Bioneer Company. Data was analyzed using the<br />SPSS software (version 16).<br /><strong>Results:</strong> There was significantly greater B-Raf proto-oncogene, serine/threonine<br />kinase expression in 82% of bladder tumor samples compared to the adjacent tissues.<br />In 91.1% of tumor samples, the gene expression was also significantly higher than healthy<br />bladder tissues from patients with benign prostatic hyperplasia. We observed<br />overexpression of B-Raf proto-oncogene, serine/threonine kinase in 61.7% of the<br />healthy margin tissue samples compared to healthy bladder tissues of patients with benign<br />prostatic hyperplasia (P<0.001). Expression of ZEB2 in 52.9% of the bladder tumor<br />samples was significantly higher than healthy peripheral tissues. This increase was<br />observed in 94.1% of tumor samples compared to healthy bladder tissues of patients<br />with benign prostatic hyperplasia (P<0.001). Pearson correlation coefficient showed<br />a positive relationship between B-Raf proto-oncogene, serine/threonine kinase and ZEB2<br />in cancerous samples (r = 0.75) and healthy margin tissue samples (r = 0.49).<br /><strong>Conclusion:</strong> During the carcinogenesis process, molecular changes are seen in<br />healthy margin tissue. These molecular changes may be the reason for the high<br />recurrence rate of bladder cancer. B-Raf proto-oncogene, serine/threonine kinase can<br />potentially be a target cancer therapy in antisense technologies. |
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| ISSN: | 2008-6709 2008-6687 |