Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer
<strong>Background:</strong> Numerous molecular changes are involved in the development and<br />progression of bladder cancer. Regular follow-up of patients is crucial due to the high<br />recurrence rate of bladder cancer. The aim of this study is to determine the role of&l...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-04-01
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| Series: | Middle East Journal of Cancer |
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| Online Access: | http://mejc.sums.ac.ir/article_44857_ca0e836ee29d928a78cbd757af6faf06.pdf |
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| author | Shima Hosseini Massoud Saidijam Hamid Eslami Ali Reza Soltanian Seyed Habibollah Mousavi-Bahar Ali Mahdavinezhad |
| author_facet | Shima Hosseini Massoud Saidijam Hamid Eslami Ali Reza Soltanian Seyed Habibollah Mousavi-Bahar Ali Mahdavinezhad |
| author_sort | Shima Hosseini |
| collection | DOAJ |
| description | <strong>Background:</strong> Numerous molecular changes are involved in the development and<br />progression of bladder cancer. Regular follow-up of patients is crucial due to the high<br />recurrence rate of bladder cancer. The aim of this study is to determine the role of<br />B-Raf proto-oncogene, serine/threonine kinase and ZEB2 expressions in onset and<br />progression of bladder cancer. We have also investigated their relationships to<br />pathological characteristics.<br /><strong>Methods:</strong> We conducted this case-control study on bladder cancer and its healthy<br />adjacent tissue, and normal bladder tissue from patients with benign prostatic hyperplasia.<br />After extraction of total RNA and cDNA synthesis, quantitative expression analysis was<br />performed in duplicate using real-time PCR. Changes in the gene expression were<br />calculated according to the 2(-ΔΔCt) equation. The products were confirmed by 1% agarose<br />gel electrophoresis and sequenced by Bioneer Company. Data was analyzed using the<br />SPSS software (version 16).<br /><strong>Results:</strong> There was significantly greater B-Raf proto-oncogene, serine/threonine<br />kinase expression in 82% of bladder tumor samples compared to the adjacent tissues.<br />In 91.1% of tumor samples, the gene expression was also significantly higher than healthy<br />bladder tissues from patients with benign prostatic hyperplasia. We observed<br />overexpression of B-Raf proto-oncogene, serine/threonine kinase in 61.7% of the<br />healthy margin tissue samples compared to healthy bladder tissues of patients with benign<br />prostatic hyperplasia (P<0.001). Expression of ZEB2 in 52.9% of the bladder tumor<br />samples was significantly higher than healthy peripheral tissues. This increase was<br />observed in 94.1% of tumor samples compared to healthy bladder tissues of patients<br />with benign prostatic hyperplasia (P<0.001). Pearson correlation coefficient showed<br />a positive relationship between B-Raf proto-oncogene, serine/threonine kinase and ZEB2<br />in cancerous samples (r = 0.75) and healthy margin tissue samples (r = 0.49).<br /><strong>Conclusion:</strong> During the carcinogenesis process, molecular changes are seen in<br />healthy margin tissue. These molecular changes may be the reason for the high<br />recurrence rate of bladder cancer. B-Raf proto-oncogene, serine/threonine kinase can<br />potentially be a target cancer therapy in antisense technologies. |
| first_indexed | 2024-12-22T05:33:06Z |
| format | Article |
| id | doaj.art-c7797c6242e34c29a0d5e383cce4d881 |
| institution | Directory Open Access Journal |
| issn | 2008-6709 2008-6687 |
| language | English |
| last_indexed | 2024-12-22T05:33:06Z |
| publishDate | 2019-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Middle East Journal of Cancer |
| spelling | doaj.art-c7797c6242e34c29a0d5e383cce4d8812022-12-21T18:37:25ZengShiraz University of Medical SciencesMiddle East Journal of Cancer2008-67092008-66872019-04-01102879410.30476/mejc.2019.81572.102644857Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder CancerShima Hosseini0Massoud Saidijam1Hamid Eslami2Ali Reza Soltanian3Seyed Habibollah Mousavi-Bahar4Ali Mahdavinezhad5Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IranModeling of Non-communicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, IranUrology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran<strong>Background:</strong> Numerous molecular changes are involved in the development and<br />progression of bladder cancer. Regular follow-up of patients is crucial due to the high<br />recurrence rate of bladder cancer. The aim of this study is to determine the role of<br />B-Raf proto-oncogene, serine/threonine kinase and ZEB2 expressions in onset and<br />progression of bladder cancer. We have also investigated their relationships to<br />pathological characteristics.<br /><strong>Methods:</strong> We conducted this case-control study on bladder cancer and its healthy<br />adjacent tissue, and normal bladder tissue from patients with benign prostatic hyperplasia.<br />After extraction of total RNA and cDNA synthesis, quantitative expression analysis was<br />performed in duplicate using real-time PCR. Changes in the gene expression were<br />calculated according to the 2(-ΔΔCt) equation. The products were confirmed by 1% agarose<br />gel electrophoresis and sequenced by Bioneer Company. Data was analyzed using the<br />SPSS software (version 16).<br /><strong>Results:</strong> There was significantly greater B-Raf proto-oncogene, serine/threonine<br />kinase expression in 82% of bladder tumor samples compared to the adjacent tissues.<br />In 91.1% of tumor samples, the gene expression was also significantly higher than healthy<br />bladder tissues from patients with benign prostatic hyperplasia. We observed<br />overexpression of B-Raf proto-oncogene, serine/threonine kinase in 61.7% of the<br />healthy margin tissue samples compared to healthy bladder tissues of patients with benign<br />prostatic hyperplasia (P<0.001). Expression of ZEB2 in 52.9% of the bladder tumor<br />samples was significantly higher than healthy peripheral tissues. This increase was<br />observed in 94.1% of tumor samples compared to healthy bladder tissues of patients<br />with benign prostatic hyperplasia (P<0.001). Pearson correlation coefficient showed<br />a positive relationship between B-Raf proto-oncogene, serine/threonine kinase and ZEB2<br />in cancerous samples (r = 0.75) and healthy margin tissue samples (r = 0.49).<br /><strong>Conclusion:</strong> During the carcinogenesis process, molecular changes are seen in<br />healthy margin tissue. These molecular changes may be the reason for the high<br />recurrence rate of bladder cancer. B-Raf proto-oncogene, serine/threonine kinase can<br />potentially be a target cancer therapy in antisense technologies.http://mejc.sums.ac.ir/article_44857_ca0e836ee29d928a78cbd757af6faf06.pdfBiomarkersBRAFZEB2Urinary bladder neoplasm |
| spellingShingle | Shima Hosseini Massoud Saidijam Hamid Eslami Ali Reza Soltanian Seyed Habibollah Mousavi-Bahar Ali Mahdavinezhad Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer Middle East Journal of Cancer Biomarkers BRAF ZEB2 Urinary bladder neoplasm |
| title | Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer |
| title_full | Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer |
| title_fullStr | Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer |
| title_full_unstemmed | Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer |
| title_short | Clinical Significance of BRAF and ZEB2 Expressions in Healthy Adjacent Tissue of Bladder Cancer |
| title_sort | clinical significance of braf and zeb2 expressions in healthy adjacent tissue of bladder cancer |
| topic | Biomarkers BRAF ZEB2 Urinary bladder neoplasm |
| url | http://mejc.sums.ac.ir/article_44857_ca0e836ee29d928a78cbd757af6faf06.pdf |
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