The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis
Background: Recent evidence suggests that the interleukin-33/interleukin-1 receptor-like 1 (IL-33/IL1RL1) axis plays a critical role in autoimmune and inflammatory disorders; however, its mechanistic role in these diseases has not been clearly defined. The present study aimed to investigate the freq...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2016-02-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/5471 |
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| author | Maryam Ahmadi Nahid Eskandari Fereshteh Ale-Sahebfosoul Mansour Salehi Ramin Ghasemi |
| author_facet | Maryam Ahmadi Nahid Eskandari Fereshteh Ale-Sahebfosoul Mansour Salehi Ramin Ghasemi |
| author_sort | Maryam Ahmadi |
| collection | DOAJ |
| description | Background: Recent evidence suggests that the interleukin-33/interleukin-1 receptor-like 1 (IL-33/IL1RL1) axis plays a critical role in autoimmune and inflammatory disorders; however, its mechanistic role in these diseases has not been clearly defined. The present study aimed to investigate the frequency of rs1342326 polymorphism of IL-33 gene and rs10204137 polymorphism of IL1RL1 gene with serum levels of IL-33 in multiple sclerosis (MS), asthma and healthy subjects In Isfahan Province, Iran.
Methods: Samples were taken from the patients with asthma (140) or multiple sclerosis (140) and healthy controls (72). Different genotypes of T/G or G/A polymorphisms were studied using the high-resolution melting real-time polymerase chain reaction (HRM real-time PCR) technique. Serum levels of IL-33 were measured via enzyme-linked immunosorbent assay (ELISA).
Findings: There was not any relationship between the frequency of polymorphism rs10204137 of IL1RL1 gene and susceptibility to multiple sclerosis or asthma. There was no significant correlation between the frequency of rs1342326 polymorphism of IL-33 gene in healthy subjects compared to those with asthma or multiple sclerosis. Serum levels of IL-33 were higher than in patients with multiple sclerosis or asthma compared to healthy subjects.
Conclusion: Serum levels of IL-33 in the two groups of patient increased substantially compared to the controls. |
| first_indexed | 2024-03-12T19:29:00Z |
| format | Article |
| id | doaj.art-c77a60096bb0496b9b0fc13c9b02b324 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T19:29:00Z |
| publishDate | 2016-02-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-c77a60096bb0496b9b0fc13c9b02b3242023-08-02T04:42:56ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2016-02-0133361209221012088The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple SclerosisMaryam Ahmadi0Nahid Eskandari1Fereshteh Ale-Sahebfosoul2Mansour Salehi3Ramin Ghasemi4MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAsthma and Allergy Specialist, Isfahan Asthma and Allergy Clinic, Isfahan, IranBackground: Recent evidence suggests that the interleukin-33/interleukin-1 receptor-like 1 (IL-33/IL1RL1) axis plays a critical role in autoimmune and inflammatory disorders; however, its mechanistic role in these diseases has not been clearly defined. The present study aimed to investigate the frequency of rs1342326 polymorphism of IL-33 gene and rs10204137 polymorphism of IL1RL1 gene with serum levels of IL-33 in multiple sclerosis (MS), asthma and healthy subjects In Isfahan Province, Iran. Methods: Samples were taken from the patients with asthma (140) or multiple sclerosis (140) and healthy controls (72). Different genotypes of T/G or G/A polymorphisms were studied using the high-resolution melting real-time polymerase chain reaction (HRM real-time PCR) technique. Serum levels of IL-33 were measured via enzyme-linked immunosorbent assay (ELISA). Findings: There was not any relationship between the frequency of polymorphism rs10204137 of IL1RL1 gene and susceptibility to multiple sclerosis or asthma. There was no significant correlation between the frequency of rs1342326 polymorphism of IL-33 gene in healthy subjects compared to those with asthma or multiple sclerosis. Serum levels of IL-33 were higher than in patients with multiple sclerosis or asthma compared to healthy subjects. Conclusion: Serum levels of IL-33 in the two groups of patient increased substantially compared to the controls.http://jims.mui.ac.ir/index.php/jims/article/view/5471Interleukin-33 (IL-33)Multiple sclerosisAsthmaPolymorphism |
| spellingShingle | Maryam Ahmadi Nahid Eskandari Fereshteh Ale-Sahebfosoul Mansour Salehi Ramin Ghasemi The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis مجله دانشکده پزشکی اصفهان Interleukin-33 (IL-33) Multiple sclerosis Asthma Polymorphism |
| title | The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis |
| title_full | The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis |
| title_fullStr | The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis |
| title_full_unstemmed | The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis |
| title_short | The Relationship between the Genetic Polymorphisms of Interleukin-33 (IL-33) and Interleukin-1 Receptor-Like 1 (IL1R1) with the Levels of Interleukin-33 in Patients with Asthma and Multiple Sclerosis |
| title_sort | relationship between the genetic polymorphisms of interleukin 33 il 33 and interleukin 1 receptor like 1 il1r1 with the levels of interleukin 33 in patients with asthma and multiple sclerosis |
| topic | Interleukin-33 (IL-33) Multiple sclerosis Asthma Polymorphism |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/5471 |
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