Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis
Conjunctival sac microbiome alterations have been reported to be closely associated with many ocular diseases. However, the characteristic of conjunctival sac microbiome in allergic conjunctivitis (AC) was scarcely described. In this study, we aimed to identify the differences of the conjunctival sa...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/4/1130 |
| _version_ | 1797479070420697088 |
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| author | Hang Song Kang Xiao Hanyi Min Zhengyu Chen Qin Long |
| author_facet | Hang Song Kang Xiao Hanyi Min Zhengyu Chen Qin Long |
| author_sort | Hang Song |
| collection | DOAJ |
| description | Conjunctival sac microbiome alterations have been reported to be closely associated with many ocular diseases. However, the characteristic of conjunctival sac microbiome in allergic conjunctivitis (AC) was scarcely described. In this study, we aimed to identify the differences of the conjunctival sac microbiome composition in AC patients compared with normal controls (NCs) using high-throughput 16S rDNA sequencing metagenomic analysis. The conjunctival sac microbiome samples from 28 AC patients and 39 NC patients were collected. The V3-V4 region of 16S rRNA gene high-throughput sequencing was performed on the illumina MiSeq platform. Alpha diversity, beta diversity and the relative abundance at the phylum and genus levels were analyzed using QIIME. Alpha diversity demonstrated by Chao1, Observed_species and PD_whole_tree indexes did not show significant difference between the AC and NC groups, while the Shannon index was higher in the AC group. Beta diversity showed divergent microbiome composition in different groups (<i>p</i> < 0.005). The top five abundant phyla were Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota and Cyanobacteria in both groups. The top five abundant genera were <i>Bacillus, Staphylococcus</i>, <i>Corynebacterium, Acinetobacter</i> and <i>Ralstonia</i> in the AC group and <i>Acinetobacter</i>, <i>Staphylococcus</i>, <i>Bacillus</i>, <i>Clostridium_sensu_stricto_1</i>, <i>Corynebacterium</i> and <i>Geobacillus</i> in the NC group. The Firmicutes/Bacteroidetes (F/B) ratio at the phylum level was similar between groups (<i>p</i> = 0.144). The <i>Bacillus/Acinetobacter</i> (<i>B/A</i>) ratio at the genus level was higher in the AC group (<i>p</i> = 0.021). The dysbiosis detected in this study might provide further evidence to investigate the mechanism and treatment methods for allergic conjunctivitis. |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T21:40:34Z |
| publishDate | 2022-02-01 |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-c77b049e188d4f33951db64791e61c1e2023-11-23T20:32:12ZengMDPI AGJournal of Clinical Medicine2077-03832022-02-01114113010.3390/jcm11041130Characterization of Conjunctival Sac Microbiome from Patients with Allergic ConjunctivitisHang Song0Kang Xiao1Hanyi Min2Zhengyu Chen3Qin Long4Department of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, ChinaDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, ChinaDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, ChinaDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, ChinaDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, ChinaConjunctival sac microbiome alterations have been reported to be closely associated with many ocular diseases. However, the characteristic of conjunctival sac microbiome in allergic conjunctivitis (AC) was scarcely described. In this study, we aimed to identify the differences of the conjunctival sac microbiome composition in AC patients compared with normal controls (NCs) using high-throughput 16S rDNA sequencing metagenomic analysis. The conjunctival sac microbiome samples from 28 AC patients and 39 NC patients were collected. The V3-V4 region of 16S rRNA gene high-throughput sequencing was performed on the illumina MiSeq platform. Alpha diversity, beta diversity and the relative abundance at the phylum and genus levels were analyzed using QIIME. Alpha diversity demonstrated by Chao1, Observed_species and PD_whole_tree indexes did not show significant difference between the AC and NC groups, while the Shannon index was higher in the AC group. Beta diversity showed divergent microbiome composition in different groups (<i>p</i> < 0.005). The top five abundant phyla were Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota and Cyanobacteria in both groups. The top five abundant genera were <i>Bacillus, Staphylococcus</i>, <i>Corynebacterium, Acinetobacter</i> and <i>Ralstonia</i> in the AC group and <i>Acinetobacter</i>, <i>Staphylococcus</i>, <i>Bacillus</i>, <i>Clostridium_sensu_stricto_1</i>, <i>Corynebacterium</i> and <i>Geobacillus</i> in the NC group. The Firmicutes/Bacteroidetes (F/B) ratio at the phylum level was similar between groups (<i>p</i> = 0.144). The <i>Bacillus/Acinetobacter</i> (<i>B/A</i>) ratio at the genus level was higher in the AC group (<i>p</i> = 0.021). The dysbiosis detected in this study might provide further evidence to investigate the mechanism and treatment methods for allergic conjunctivitis.https://www.mdpi.com/2077-0383/11/4/1130conjunctival sacmicrobial diversitymicrobial compositionallergic conjunctivitis |
| spellingShingle | Hang Song Kang Xiao Hanyi Min Zhengyu Chen Qin Long Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis Journal of Clinical Medicine conjunctival sac microbial diversity microbial composition allergic conjunctivitis |
| title | Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis |
| title_full | Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis |
| title_fullStr | Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis |
| title_full_unstemmed | Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis |
| title_short | Characterization of Conjunctival Sac Microbiome from Patients with Allergic Conjunctivitis |
| title_sort | characterization of conjunctival sac microbiome from patients with allergic conjunctivitis |
| topic | conjunctival sac microbial diversity microbial composition allergic conjunctivitis |
| url | https://www.mdpi.com/2077-0383/11/4/1130 |
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