Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.)
Spring regrowth is an important trait for perennial plants including alfalfa, the most cultivated forage legume worldwide. However, the genetic and genomic basis of the trait is largely unknown in alfalfa due to its complex genetic background of the tetroploid genome. The objective of this study was...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636717 |
| _version_ | 1798024163959504896 |
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| author | Xue-qian JIANG Fan ZHANG Zhen WANG Rui-cai LONG Ming-na LI Fei HE Xi-jiang YANG Chang-fu YANG Xu JIANG Qing-chuan YANG Quan-zhen WANG Jun-mei KANG |
| author_facet | Xue-qian JIANG Fan ZHANG Zhen WANG Rui-cai LONG Ming-na LI Fei HE Xi-jiang YANG Chang-fu YANG Xu JIANG Qing-chuan YANG Quan-zhen WANG Jun-mei KANG |
| author_sort | Xue-qian JIANG |
| collection | DOAJ |
| description | Spring regrowth is an important trait for perennial plants including alfalfa, the most cultivated forage legume worldwide. However, the genetic and genomic basis of the trait is largely unknown in alfalfa due to its complex genetic background of the tetroploid genome. The objective of this study was to identify quantitative trait loci (QTLs) associated with spring regrowth using high-resolution genetic linkage maps we constructed previously. In total, 36 significant additive effect QTLs for the trait were detected. Among them, 10 QTLs individually explained more than 10% of the phenotypic variation (PVE) with four in P1 and six in P2. Six overlapped QTLs intervals were detected with two and four intervals distributed in P1 and P2, respectively. In P1, both overlapped genomic regions were located on homolog 7D. In P2, the four QTLs with PVE>10% were co-localized on homolog 6D. Meanwhile, six pairs of significant epistatic QTLs were identified in P2. Screening of potential candidate genes associated with four overlapped QTLs (qCP2019-8, qLF2019-5, qLF2020-4, and qBLUP-3) narrowed down one candidate annotated as MAIL1. The Arabidopsis homolog gene has been reported to play an important role in plant growth. Therefore, the detected QTLs are valuable resources for genetic improvement of alfalfa spring vigor using marker-assisted selection (MAS), and further identification of the associated genes would provide insights into genetic control of spring regrowth in alfalfa. |
| first_indexed | 2024-04-11T17:58:03Z |
| format | Article |
| id | doaj.art-c77b2b681d1548999b7ed5210ea594c5 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-11T17:58:03Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-c77b2b681d1548999b7ed5210ea594c52022-12-22T04:10:36ZengElsevierJournal of Integrative Agriculture2095-31192022-03-01213812818Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.)Xue-qian JIANG0Fan ZHANG1Zhen WANG2Rui-cai LONG3Ming-na LI4Fei HE5Xi-jiang YANG6Chang-fu YANG7Xu JIANG8Qing-chuan YANG9Quan-zhen WANG10Jun-mei KANG11Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, P.R.ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Correspondence KANG Jun-meiSpring regrowth is an important trait for perennial plants including alfalfa, the most cultivated forage legume worldwide. However, the genetic and genomic basis of the trait is largely unknown in alfalfa due to its complex genetic background of the tetroploid genome. The objective of this study was to identify quantitative trait loci (QTLs) associated with spring regrowth using high-resolution genetic linkage maps we constructed previously. In total, 36 significant additive effect QTLs for the trait were detected. Among them, 10 QTLs individually explained more than 10% of the phenotypic variation (PVE) with four in P1 and six in P2. Six overlapped QTLs intervals were detected with two and four intervals distributed in P1 and P2, respectively. In P1, both overlapped genomic regions were located on homolog 7D. In P2, the four QTLs with PVE>10% were co-localized on homolog 6D. Meanwhile, six pairs of significant epistatic QTLs were identified in P2. Screening of potential candidate genes associated with four overlapped QTLs (qCP2019-8, qLF2019-5, qLF2020-4, and qBLUP-3) narrowed down one candidate annotated as MAIL1. The Arabidopsis homolog gene has been reported to play an important role in plant growth. Therefore, the detected QTLs are valuable resources for genetic improvement of alfalfa spring vigor using marker-assisted selection (MAS), and further identification of the associated genes would provide insights into genetic control of spring regrowth in alfalfa.http://www.sciencedirect.com/science/article/pii/S2095311921636717alfalfaGBSgenetic mapQTLspring regrowth |
| spellingShingle | Xue-qian JIANG Fan ZHANG Zhen WANG Rui-cai LONG Ming-na LI Fei HE Xi-jiang YANG Chang-fu YANG Xu JIANG Qing-chuan YANG Quan-zhen WANG Jun-mei KANG Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) Journal of Integrative Agriculture alfalfa GBS genetic map QTL spring regrowth |
| title | Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) |
| title_full | Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) |
| title_fullStr | Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) |
| title_full_unstemmed | Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) |
| title_short | Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.) |
| title_sort | detection of quantitative trait loci qtl associated with spring regrowth in alfalfa medicago sativa l |
| topic | alfalfa GBS genetic map QTL spring regrowth |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636717 |
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