Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods
Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat’s epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water). Me...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2008-03-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2008.11.1.127 |
| _version_ | 1818324584630321152 |
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| author | Jae-eun, Lee Byoung Woo, Kim Ki Rok, Kwon Tae Jin, Rhim Dong Heui Kim |
| author_facet | Jae-eun, Lee Byoung Woo, Kim Ki Rok, Kwon Tae Jin, Rhim Dong Heui Kim |
| author_sort | Jae-eun, Lee |
| collection | DOAJ |
| description | Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat’s epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water).
Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate
the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were
treated with 0.01, 0.1, 1.0㎎/㎖ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, 10.0㎎/㎖ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them.
Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues.
1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially 1.0㎎/㎖), and
especially alcohol extracts had better effects.
2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the concentrations of 0.1, 1.0㎎/㎖. Alcohol extracts had better effects than water extracts.
3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, 1.0㎎/㎖, and especially
on the concentration of 1.0㎎/㎖ alcohol extract of Chowiseungcheng-tang had better effect.
4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of 10.0㎎/㎖, but alcohol extracts of Chowiseungcheng-tang had it on all concentrations.
Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipocytes proliferation, lipolysis of adipocytes and localized fat accumulation than water extracts |
| first_indexed | 2024-12-13T11:30:55Z |
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| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-13T11:30:55Z |
| publishDate | 2008-03-01 |
| publisher | Korean Pharmacopuncture Institute |
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| series | Journal of Pharmacopuncture |
| spelling | doaj.art-c77e232ac1b4417abaa3a0b633d8bce92022-12-21T23:47:57ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562008-03-0111112714110.3831/KPI.2008.11.1.127Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methodsJae-eun, Lee0Byoung Woo, Kim1Ki Rok, Kwon2Tae Jin, Rhim3Dong Heui Kim4Department of Internal Medicine, Korean Medical College, Sangji UniversityDepartment of Internal Medicine, Korean Medical College, Sangji UniversityDepartment of Acup & Moxi, Korean Medical College, Sangji UniversityDevision of Animal resourses and life science, Sangji UniversityDepartment of Environmental Medical Biology, Wonju Medical College, Yonsei UniversityObjectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat’s epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water). Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, 1.0㎎/㎖ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, 10.0㎎/㎖ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them. Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially 1.0㎎/㎖), and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the concentrations of 0.1, 1.0㎎/㎖. Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, 1.0㎎/㎖, and especially on the concentration of 1.0㎎/㎖ alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of 10.0㎎/㎖, but alcohol extracts of Chowiseungcheng-tang had it on all concentrations. Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipocytes proliferation, lipolysis of adipocytes and localized fat accumulation than water extractshttp://dx.doi.org/10.3831/KPI.2008.11.1.127Chowiseungcheng-tangAlcohol extractsWaterextractsPreadipocytesProliferationAdipocytesLipolysisFat tissues |
| spellingShingle | Jae-eun, Lee Byoung Woo, Kim Ki Rok, Kwon Tae Jin, Rhim Dong Heui Kim Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods Journal of Pharmacopuncture Chowiseungcheng-tang Alcohol extracts Waterextracts Preadipocytes Proliferation Adipocytes Lipolysis Fat tissues |
| title | Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods |
| title_full | Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods |
| title_fullStr | Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods |
| title_full_unstemmed | Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods |
| title_short | Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods |
| title_sort | effects of chowiseungcheng tang extracts on the preadipocytes proliferation in 3t3 l1 cell line lipolysis of adipocytes in rat and localized fat accumulation by extraction methods |
| topic | Chowiseungcheng-tang Alcohol extracts Waterextracts Preadipocytes Proliferation Adipocytes Lipolysis Fat tissues |
| url | http://dx.doi.org/10.3831/KPI.2008.11.1.127 |
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