Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing
The relationship between the cervico-vaginal microbiome and high-risk human papillomavirus (HR-HPV) is well observed. However, there is a lack of adequate research regarding the cervical microbiota in HR-HPV infection. Most published research results have used 16S rRNA gene sequencing technology; th...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.922554/full |
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| author | Bingyan Fang Bingyan Fang Qun Li Zixian Wan Zixian Wan Zhenbo OuYang Qiushi Zhang Qiushi Zhang |
| author_facet | Bingyan Fang Bingyan Fang Qun Li Zixian Wan Zixian Wan Zhenbo OuYang Qiushi Zhang Qiushi Zhang |
| author_sort | Bingyan Fang |
| collection | DOAJ |
| description | The relationship between the cervico-vaginal microbiome and high-risk human papillomavirus (HR-HPV) is well observed. However, there is a lack of adequate research regarding the cervical microbiota in HR-HPV infection. Most published research results have used 16S rRNA gene sequencing technology; this technology only focuses on marker sequences, resulting in incomplete gene information acquisition. Metagenomic sequencing technology can effectively compensate for the deficiency of 16S rRNA gene sequencing, thus improving the analysis of microbiota function. Cervical swab samples from 20 females with HR-HPV infection and 20 uninfected (Control) women were analyzed through 16S rRNA gene and metagenomic sequencing. Our results indicated that the composition and function of the cervical microbiota of HR-HPV infection differed notably from that of control women. Compared with control women, Firmicutes was decreased during HR-HPV infection, whereas Actinobacteria was increased. At the genus level, Lactobacillus was enriched in control women, while levels of Gardnerella and Bifidobacterium were lower. At the species level, Lactobacillus crispatus, L. jensenii, and L. helveticus were enriched in control women; these were the top three species with biomarker significance between the two groups. Eight pathways and four KEGG orthologies of the cervical microbiota of statistical differences were identified between the HR-HPV infection and control women. Collectively, our study described the cervical microbiota and its potential function during HR-HPV infection. Biomarkers of cervical microbiota and the changed bacterial metabolic pathways and metabolites can help clarify the pathogenic mechanism of HR-HPV infection, making them promising targets for clinical treatment and intervention for HR-HPV infection and cervical carcinoma. |
| first_indexed | 2024-04-13T19:09:41Z |
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| id | doaj.art-c77e9603876e404aa3f71b21e6b9a93c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T19:09:41Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c77e9603876e404aa3f71b21e6b9a93c2022-12-22T02:33:53ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.922554922554Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic SequencingBingyan Fang0Bingyan Fang1Qun Li2Zixian Wan3Zixian Wan4Zhenbo OuYang5Qiushi Zhang6Qiushi Zhang7The Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Gynecology, Guangdong Second Provincial General Hospital, Guangzhou, ChinaGraduate Collaborative Training Base of Guangdong Second Provincial General Hospital, Hengyang Medical School, University of South China, Hengyang, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Gynecology, Guangdong Second Provincial General Hospital, Guangzhou, ChinaDepartment of Gynecology, Guangdong Second Provincial General Hospital, Guangzhou, ChinaDepartment of Gynecology, Guangdong Second Provincial General Hospital, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaThe relationship between the cervico-vaginal microbiome and high-risk human papillomavirus (HR-HPV) is well observed. However, there is a lack of adequate research regarding the cervical microbiota in HR-HPV infection. Most published research results have used 16S rRNA gene sequencing technology; this technology only focuses on marker sequences, resulting in incomplete gene information acquisition. Metagenomic sequencing technology can effectively compensate for the deficiency of 16S rRNA gene sequencing, thus improving the analysis of microbiota function. Cervical swab samples from 20 females with HR-HPV infection and 20 uninfected (Control) women were analyzed through 16S rRNA gene and metagenomic sequencing. Our results indicated that the composition and function of the cervical microbiota of HR-HPV infection differed notably from that of control women. Compared with control women, Firmicutes was decreased during HR-HPV infection, whereas Actinobacteria was increased. At the genus level, Lactobacillus was enriched in control women, while levels of Gardnerella and Bifidobacterium were lower. At the species level, Lactobacillus crispatus, L. jensenii, and L. helveticus were enriched in control women; these were the top three species with biomarker significance between the two groups. Eight pathways and four KEGG orthologies of the cervical microbiota of statistical differences were identified between the HR-HPV infection and control women. Collectively, our study described the cervical microbiota and its potential function during HR-HPV infection. Biomarkers of cervical microbiota and the changed bacterial metabolic pathways and metabolites can help clarify the pathogenic mechanism of HR-HPV infection, making them promising targets for clinical treatment and intervention for HR-HPV infection and cervical carcinoma.https://www.frontiersin.org/articles/10.3389/fcimb.2022.922554/full16S rRNA gene sequencingmetagenomic sequencingHR-HPVcervical microbiotacancer |
| spellingShingle | Bingyan Fang Bingyan Fang Qun Li Zixian Wan Zixian Wan Zhenbo OuYang Qiushi Zhang Qiushi Zhang Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing Frontiers in Cellular and Infection Microbiology 16S rRNA gene sequencing metagenomic sequencing HR-HPV cervical microbiota cancer |
| title | Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing |
| title_full | Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing |
| title_fullStr | Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing |
| title_full_unstemmed | Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing |
| title_short | Exploring the Association Between Cervical Microbiota and HR-HPV Infection Based on 16S rRNA Gene and Metagenomic Sequencing |
| title_sort | exploring the association between cervical microbiota and hr hpv infection based on 16s rrna gene and metagenomic sequencing |
| topic | 16S rRNA gene sequencing metagenomic sequencing HR-HPV cervical microbiota cancer |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.922554/full |
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