Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts
The microbial synthesis of nanoparticles is a green chemistry approach that combines nanotechnology and microbial biotechnology. The aim of this study was to obtain silver nanoparticles (SNPs) using aqueous extract from the filamentous fungus Fusarium oxysporum as an alternative to chemical procedur...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200220&lng=en&tlng=en |
| _version_ | 1797704752819077120 |
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| author | Kelly Ishida Talita Ferreira Cipriano Gustavo Miranda Rocha Gilberto Weissmüller Fabio Gomes Kildare Miranda Sonia Rozental |
| author_facet | Kelly Ishida Talita Ferreira Cipriano Gustavo Miranda Rocha Gilberto Weissmüller Fabio Gomes Kildare Miranda Sonia Rozental |
| author_sort | Kelly Ishida |
| collection | DOAJ |
| description | The microbial synthesis of nanoparticles is a green chemistry approach that combines nanotechnology and microbial biotechnology. The aim of this study was to obtain silver nanoparticles (SNPs) using aqueous extract from the filamentous fungus Fusarium oxysporum as an alternative to chemical procedures and to evaluate its antifungal activity. SNPs production increased in a concentration-dependent way up to 1 mM silver nitrate until 30 days of reaction. Monodispersed and spherical SNPs were predominantly produced. After 60 days, it was possible to observe degenerated SNPs with in additional needle morphology. The SNPs showed a high antifungal activity against Candida and Cryptococcus , with minimum inhibitory concentration values ≤ 1.68 µg/mL for both genera. Morphological alterations of Cryptococcus neoformans treated with SNPs were observed such as disruption of the cell wall and cytoplasmic membrane and lost of the cytoplasm content. This work revealed that SNPs can be easily produced by F. oxysporum aqueous extracts and may be a feasible, low-cost, environmentally friendly method for generating stable and uniformly sized SNPs. Finally, we have demonstrated that these SNPs are active against pathogenic fungi, such as Candida and Cryptococcus . |
| first_indexed | 2024-03-12T05:25:04Z |
| format | Article |
| id | doaj.art-c77fe478852f4043a953256dcd1c96dd |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:04Z |
| publishDate | 2014-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c77fe478852f4043a953256dcd1c96dd2023-09-03T07:25:04ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-04-01109222022810.1590/0074-0276130269S0074-02762014000200220Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeastsKelly IshidaTalita Ferreira CiprianoGustavo Miranda RochaGilberto WeissmüllerFabio GomesKildare MirandaSonia RozentalThe microbial synthesis of nanoparticles is a green chemistry approach that combines nanotechnology and microbial biotechnology. The aim of this study was to obtain silver nanoparticles (SNPs) using aqueous extract from the filamentous fungus Fusarium oxysporum as an alternative to chemical procedures and to evaluate its antifungal activity. SNPs production increased in a concentration-dependent way up to 1 mM silver nitrate until 30 days of reaction. Monodispersed and spherical SNPs were predominantly produced. After 60 days, it was possible to observe degenerated SNPs with in additional needle morphology. The SNPs showed a high antifungal activity against Candida and Cryptococcus , with minimum inhibitory concentration values ≤ 1.68 µg/mL for both genera. Morphological alterations of Cryptococcus neoformans treated with SNPs were observed such as disruption of the cell wall and cytoplasmic membrane and lost of the cytoplasm content. This work revealed that SNPs can be easily produced by F. oxysporum aqueous extracts and may be a feasible, low-cost, environmentally friendly method for generating stable and uniformly sized SNPs. Finally, we have demonstrated that these SNPs are active against pathogenic fungi, such as Candida and Cryptococcus .http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200220&lng=en&tlng=ensilver nanoparticlemicroscopyenvironmentally friendly procedureantifungalCandidaCryptococcus |
| spellingShingle | Kelly Ishida Talita Ferreira Cipriano Gustavo Miranda Rocha Gilberto Weissmüller Fabio Gomes Kildare Miranda Sonia Rozental Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts Memorias do Instituto Oswaldo Cruz silver nanoparticle microscopy environmentally friendly procedure antifungal Candida Cryptococcus |
| title | Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| title_full | Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| title_fullStr | Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| title_full_unstemmed | Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| title_short | Silver nanoparticle production by the fungus Fusarium oxysporum: nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| title_sort | silver nanoparticle production by the fungus fusarium oxysporum nanoparticle characterisation and analysis of antifungal activity against pathogenic yeasts |
| topic | silver nanoparticle microscopy environmentally friendly procedure antifungal Candida Cryptococcus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200220&lng=en&tlng=en |
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