Challenges of CRISPR-Based Gene Editing in Primary T Cells
Adaptive T-cell immunotherapy holds great promise for the successful treatment of leukemia, as well as other types of cancers. More recently, it was also shown to be an effective treatment option for chronic virus infections in immunosuppressed patients. Autologous or allogeneic T cells used for imm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1689 |
| _version_ | 1797487263349735424 |
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| author | Alaleh Rezalotfi Lea Fritz Reinhold Förster Berislav Bošnjak |
| author_facet | Alaleh Rezalotfi Lea Fritz Reinhold Förster Berislav Bošnjak |
| author_sort | Alaleh Rezalotfi |
| collection | DOAJ |
| description | Adaptive T-cell immunotherapy holds great promise for the successful treatment of leukemia, as well as other types of cancers. More recently, it was also shown to be an effective treatment option for chronic virus infections in immunosuppressed patients. Autologous or allogeneic T cells used for immunotherapy are usually genetically modified to express novel T-cell or chimeric antigen receptors. The production of such cells was significantly simplified with the CRISPR/Cas system, allowing for the deletion or insertion of novel genes at specific locations within the genome. In this review, we describe recent methodological breakthroughs that were important for the conduction of these genetic modifications, summarize crucial points to be considered when conducting such experiments, and highlight the potential pitfalls of these approaches. |
| first_indexed | 2024-03-09T23:45:08Z |
| format | Article |
| id | doaj.art-c78344b7a7f941db8c24699cc35c6e35 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:45:08Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c78344b7a7f941db8c24699cc35c6e352023-11-23T16:44:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01233168910.3390/ijms23031689Challenges of CRISPR-Based Gene Editing in Primary T CellsAlaleh Rezalotfi0Lea Fritz1Reinhold Förster2Berislav Bošnjak3Institute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyAdaptive T-cell immunotherapy holds great promise for the successful treatment of leukemia, as well as other types of cancers. More recently, it was also shown to be an effective treatment option for chronic virus infections in immunosuppressed patients. Autologous or allogeneic T cells used for immunotherapy are usually genetically modified to express novel T-cell or chimeric antigen receptors. The production of such cells was significantly simplified with the CRISPR/Cas system, allowing for the deletion or insertion of novel genes at specific locations within the genome. In this review, we describe recent methodological breakthroughs that were important for the conduction of these genetic modifications, summarize crucial points to be considered when conducting such experiments, and highlight the potential pitfalls of these approaches.https://www.mdpi.com/1422-0067/23/3/1689adoptive T-cell therapyCAR T cellsCRISPR/Cas9gene modificationsT cells |
| spellingShingle | Alaleh Rezalotfi Lea Fritz Reinhold Förster Berislav Bošnjak Challenges of CRISPR-Based Gene Editing in Primary T Cells International Journal of Molecular Sciences adoptive T-cell therapy CAR T cells CRISPR/Cas9 gene modifications T cells |
| title | Challenges of CRISPR-Based Gene Editing in Primary T Cells |
| title_full | Challenges of CRISPR-Based Gene Editing in Primary T Cells |
| title_fullStr | Challenges of CRISPR-Based Gene Editing in Primary T Cells |
| title_full_unstemmed | Challenges of CRISPR-Based Gene Editing in Primary T Cells |
| title_short | Challenges of CRISPR-Based Gene Editing in Primary T Cells |
| title_sort | challenges of crispr based gene editing in primary t cells |
| topic | adoptive T-cell therapy CAR T cells CRISPR/Cas9 gene modifications T cells |
| url | https://www.mdpi.com/1422-0067/23/3/1689 |
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